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31.发明申请Process for detecting the presence or quantity of enzymatic activity in a sample 有权用于检测样品中酶活性的存在或数量的方法公开(公告)号：US20040192893A1
公开(公告)日：2004-09-30
申请号：US10764417
申请日：2004-01-23
IPC分类号： C07K014/54 , C07H021/04 , C07K016/46
CPC分类号： C07H21/00 , C09B23/02 , C09B47/00 , C09B57/00 , Y10S436/80
摘要： This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.
摘要翻译： 本发明提供标记试剂，标记的靶标和制备标记试剂的方法。 标记试剂可以采用花青染料，呫吨染料，卟啉染料，香豆素染料或复合染料的形式。 这些标记试剂可用于标记探针或靶标，包括核酸和蛋白质。 这些试剂可用于基于蛋白质和基于核酸探针的测定。 它们也适用于实时检测过程。
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公开(公告)号：US20040176586A1
公开(公告)日：2004-09-09
申请号：US10764418
申请日：2004-01-23
IPC分类号： C07H021/04 , C07H015/24 , C07K014/705 , C07K014/52
CPC分类号： C07H21/00 , C09B23/02 , C09B47/00 , C09B57/00 , Y10S436/80
摘要： This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.
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公开(公告)号：US20220034897A1
公开(公告)日：2022-02-03
申请号：US17501143
申请日：2021-10-14
摘要： Provided are methods and compositions which are useful for separating, isolating, detecting, and quantifying compounds of interest which have been modified chemically, enzymatically or catalytically from other compounds which have not been so modified. The modifications may take the form of functional groups which are gained, lost or retained by the compounds of interest.
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公开(公告)号：US20210079034A1
公开(公告)日：2021-03-18
申请号：US16952399
申请日：2020-11-19
发明人： Zaiguo Li , Praveen Pande
摘要： Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.
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公开(公告)号：US10648013B2
公开(公告)日：2020-05-12
申请号：US16387992
申请日：2019-04-18
IPC分类号： G01N33/573 , C12Q1/37 , C07D493/10 , C12Q1/34 , C07D311/16
摘要： Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine.
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公开(公告)号：US10308974B2
公开(公告)日：2019-06-04
申请号：US15944336
申请日：2018-04-03
IPC分类号： C07D311/16 , C12Q1/34 , C07D493/10
摘要： Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine.
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公开(公告)号：US20190119719A1
公开(公告)日：2019-04-25
申请号：US16177676
申请日：2018-11-01
IPC分类号： C12Q1/26 , C12Q1/48 , C07D311/08 , C07D491/052 , C07D491/20 , C09K11/06 , C12Q1/44 , C12Q1/37 , C07D493/10
摘要： Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.
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38.发明授权公开(公告)号：US10144956B2
公开(公告)日：2018-12-04
申请号：US15054462
申请日：2016-02-26
IPC分类号： C12Q1/68 , C12Q1/6818 , C12Q1/686 , C12Q1/6844 , G01N21/64 , C12Q1/6834 , C12Q1/6853
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
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公开(公告)号：US20170137863A1
公开(公告)日：2017-05-18
申请号：US15416119
申请日：2017-01-26
IPC分类号： C12Q1/26 , C09K11/06 , C07D491/052 , C07D491/20 , C07D493/10 , C07D311/08
CPC分类号： C12Q1/26 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10 , C09K11/06 , C09K2211/1022 , C09K2211/1088 , C12Q1/37 , C12Q1/44 , C12Q1/485 , G01N2333/902 , G01N2500/00 , Y10T436/18
摘要： Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.
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公开(公告)号：US20170122954A1
公开(公告)日：2017-05-04
申请号：US15402505
申请日：2017-01-10
CPC分类号： G01N33/582 , C12Q1/02 , G01N15/14 , G01N33/5005
摘要： Provided are methods, kits and systems for simultaneously profiling of global reactive species and selected reactive species, such as global and specific reactive oxygen species (ROS), reactive nitrogen species (RNS), reactive halogen species (RHS) or combinations thereof through multiplexed fluorescence detection of three or more compatible indicator probes in live cells or subcellular organelles.
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