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88 条记录 (耗时 0.03 秒)

    Use of hexahydrolupulones as antibacterial agents
    31.
    发明授权
    Use of hexahydrolupulones as antibacterial agents 有权
    官能化硅化合物及其合成和使用方法

    公开(公告)号：US06486286B1

    公开(公告)日：2002-11-26

    申请号：US09418044

    申请日：1999-10-13

    申请人： Glenn McGall ,  Jonathan Eric Forman

    发明人： Glenn McGall ,  Jonathan Eric Forman

    IPC分类号： C08G7700

    CPC分类号： C08G73/0206 ,  C03C17/30 ,  C03C2218/113 ,  C07F7/1804 ,  C09D179/02 ,  C09D183/08 ,  C09D183/14 ,  Y10T428/31663

    摘要： Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

    摘要翻译： 提供了官能化硅化合物及其合成和使用的方法。 官能化的硅化合物包括至少一个活化的硅基团和至少一个可衍生的官能团。 示例性的可衍生的官能团包括羟基，氨基，羧基和硫醇，以及其修饰形式，例如活化或受保护的形式。 官能化的硅化合物可以共价连接到表面以形成可用于广泛的不同应用的官能化表面。 在一个实施方案中，将硅化合物连接到包含二氧化硅（例如玻璃底物）的底物的表面上，以在基底上提供功能化的表面，分子（包括多肽和核酸）可以附着于该功能化表面。 在一个实施方案中，在官能化硅化合物共价连接到固体二氧化硅底物的表面上以在基底上形成官能化涂层之后，核酸阵列可以共价连接到基底上。 因此，该方法允许形成固定在底物上的高密度核酸阵列，其可用于例如进行高体积核酸杂交测定。

    Photolithographic solid-phase polymer synthesis
    33.
    发明授权
    Photolithographic solid-phase polymer synthesis 有权
    光刻固相聚合物合成

    公开(公告)号：US07144700B1

    公开(公告)日：2006-12-05

    申请号：US09620780

    申请日：2000-07-21

    申请人： Glenn McGall ,  Daniel E. Falvey ,  Jacqueline A. Fidanza ,  Brian M. Feldman

    发明人： Glenn McGall ,  Daniel E. Falvey ,  Jacqueline A. Fidanza ,  Brian M. Feldman

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H21/00 ,  G01N33/543

    CPC分类号： C07H21/00 ,  Y02P20/55 ,  Y10T436/17 ,  Y10T436/23

    摘要： Methods, employing a polycyclic hydrocarbon or a polycyclic heteroaromatic compound as sensitizers, are provided to increase the efficiency of removing, by irradiation, photolabile protecting groups that mask reactive sites on synthesis intermediaries. Preferred groups of photolabile protecting moieties include: ((α-methyl-2-nitropiperonyl)-oxy)carbonyl (MeNPOC), ((Phenacyl)-oxy)carbonyl (PAOC), O-(9-phenylxanthen-9-yl) (PIXYL), and ((2-methylene-9,10-anthraquinone)-oxy)carbonyl (MAQOC). In conjunction with using the sensitizers and protecting groups described above, a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support is provided. These resulting solid-phase arrays are useful, for example, to assay for the presence of biochemical products in biological samples.

    摘要翻译： 提供了使用多环烃或多环杂芳族化合物作为敏化剂的方法，以提高通过照射去除掩蔽合成中间体上反应性位点的光不稳定保护基的效率。 优选的光不稳定保护基团包括：（（α-甲基-2-硝基吡喃基） - 氧基）羰基（MeNPOC），（（苯甲酰基） - 氧基）羰基（PAOC），O-（9-苯基呫吨-9-基） PIXYL）和（（2-亚甲基-9,10-蒽醌） - 氧基）羰基（MAQOC）。 结合使用上述敏化剂和保护基团，提供了从组分分子在载体上形成多种化合物的方法，每种化合物占据支持体的单独的预定区域。 这些所得到的固相阵列可用于例如测定生物样品中生化产物的存在。

    Functionalized silicon compounds and methods for their synthesis and use
    34.
    发明授权
    Functionalized silicon compounds and methods for their synthesis and use 有权

    公开(公告)号：US07129307B2

    公开(公告)日：2006-10-31

    申请号：US10853378

    申请日：2004-05-25

    申请人： Glenn McGall ,  Jonathan Eric Forman

    发明人： Glenn McGall ,  Jonathan Eric Forman

    IPC分类号： C08G77/00 ,  C08G77/04

    CPC分类号： C08G73/0206 ,  C03C17/30 ,  C03C2218/113 ,  C07F7/1804 ,  C09D179/02 ,  C09D183/08 ,  C09D183/14 ,  Y10T428/31663

    摘要： Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

    Detection of polynucleotides on nucleic acid arrays using azido-modified triphosphate nucleotide analogs
    37.
    发明申请
    Detection of polynucleotides on nucleic acid arrays using azido-modified triphosphate nucleotide analogs 审中-公开
    使用叠氮基修饰的三磷酸核苷酸类似物检测核酸阵列上的多核苷酸

    公开(公告)号：US20060147963A1

    公开(公告)日：2006-07-06

    申请号：US11313480

    申请日：2005-12-20

    申请人： Anthony Barone ,  Handong Li ,  Glenn McGall

    发明人： Anthony Barone ,  Handong Li ,  Glenn McGall

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q2600/158 ,  C12Q2525/113 ,  C12Q2565/501 ,  C12Q2521/107 ,  C12Q2525/143 ,  C12Q2525/117

    摘要： Methods are provided for detecting hybridization of a polynucleotide to a nucleic acid array by chemically modifying the polynucleotide to contain a detectable label. According to one aspect of the present invention, a method is provided for detecting the presence of a mRNA in a nucleic acid sample, the method having the steps of providing a mRNA sample and azido modified nucleotides, hybridizing a primer to the mRNA, reversed transcribing the mRNA to provide azido modified DNA, followed by reacting the azido groups with a detectable label, hybridizing the labeled RNA to a nucleic acid array and detecting the presence of the mRNA. Still other methods are provided for detecting the presence or absence of a polynucleotide of interest on a nucleic acid array, the method having the steps of providing a nucleic acid sample comprising a polynucleotide; providing an enzyme to amplify the polynucleotide using an azido nucleotide derivative; amplifying said polynucleotide to provide azido labeled amplified nucleic acids; reacting the azido groups on said nucleic acids with a detectable label to provide labeled nucleic acids; hybridizing said amplified nucleic acids to a nucleic acid array; and detecting the presence or absence of said polynucleotide. Still other methods are presented for detecting polynucleotides on a nucleic acid array using ligases and terminal transferases to end label polynucleotides.

    摘要翻译： 提供了通过化学修饰多核苷酸以含有可检测标记物来检测多核苷酸与核酸阵列的杂交的方法。 根据本发明的一个方面，提供了一种用于检测核酸样品中mRNA的存在的方法，该方法具有以下步骤：提供mRNA样品和叠氮基修饰的核苷酸，将引物与mRNA杂交，逆转录 mRNA以提供叠氮基修饰的DNA，然后使叠氮基与可检测标记反应，将标记的RNA与核酸阵列杂交并检测mRNA的存在。 还提供了用于在核酸阵列上检测感兴趣的多核苷酸的存在或不存在的其它方法，所述方法具有提供包含多核苷酸的核酸样品的步骤; 提供使用叠氮基核苷酸衍生物扩增多核苷酸的酶; 扩增所述多核苷酸以提供叠氮基标记的扩增核酸; 使所述核酸上的叠氮基与可检测标记物反应以提供标记的核酸; 将所述扩增的核酸与核酸阵列杂交; 并检测所述多核苷酸的存在或不存在。 还提出了使用连接酶和末端转移酶终止标记多核苷酸检测核酸阵列上的多核苷酸的其它方法。