公开(公告)号：US08455714B2

公开(公告)日：2013-06-04

申请号：US13329939

申请日：2011-12-19

Applicant: Keith Roesler ,  Knut Meyer ,  Howard Glenn Damude ,  Bo Shen ,  Changjiang Li ,  Ericka Bermudez ,  Mitchell C. Tarczynski

Inventor： Keith Roesler ,  Knut Meyer ,  Howard Glenn Damude ,  Bo Shen ,  Changjiang Li ,  Ericka Bermudez ,  Mitchell C. Tarczynski

IPC: A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/82

CPC classification number: C12N15/8247 ,  A23K10/30 ,  A23L11/00 ,  A23V2002/00 ,  C12N9/1029 ,  C12Y203/0102

Abstract: Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.

Abstract translation: 描述了与无分离油籽油的总脂肪酸含量相比，具有总脂肪酸含量增加至少10％和改变的脂肪酸分布的转基因油籽。 新型DGAT基因用于实现种子储存脂质的增加。

公开(公告)号：US20120096588A1

公开(公告)日：2012-04-19

申请号：US13328677

申请日：2011-12-16

Applicant: Knut Meyer ,  Howard Glenn Damude ,  Kevin G. Ripp ,  Kevin L. Stecca

Inventor： Knut Meyer ,  Howard Glenn Damude ,  Kevin G. Ripp ,  Kevin L. Stecca

IPC: A01H5/00 ,  C12N9/10 ,  C12N15/80 ,  C12N5/10 ,  A01H5/10 ,  C12N1/19 ,  C12N15/54 ,  C12N15/82

CPC classification number: C12N15/8247 ,  C12N9/1029 ,  C12Y203/0102

Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.

Abstract translation: 描述了与非转基因，无偏分离大豆种子的总脂肪酸含量相比，具有至少10％的总脂肪酸含量增加和改变的脂肪酸分布的转基因大豆种子。 来自含油生物的DGAT基因用于实现种子储存脂质的增加。

公开(公告)号：US20120058245A1

公开(公告)日：2012-03-08

申请号：US13295316

申请日：2011-11-14

Applicant: Knut Meyer ,  Kevin L. Stecca

Inventor： Knut Meyer ,  Kevin L. Stecca

IPC: A01H5/10 ,  A01H5/00 ,  A23L1/20 ,  C12N15/82

CPC classification number: C12N15/8247 ,  A23L11/07 ,  A23V2002/00 ,  C12N9/1029 ,  C12N9/1033 ,  C12N9/1037

Abstract: Transgenic soybean seed having increased total fatty acid content of at least 20% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia lipolytica combined with plastidic phosphoglucomutase down regulation are used to achieve the increase in seed storage lipids.

Abstract translation: 描述了与非转基因，无分离大豆种子的总脂肪酸含量相比，具有至少20％的总脂肪酸含量增加和改变的脂肪酸谱的转基因大豆种子。 使用解脂耶氏酵母的DGAT基因与质体磷酸葡萄糖转运酶下调相结合，实现种子储存脂质的增加。

公开(公告)号：US07918213B2

公开(公告)日：2011-04-05

申请号：US12046156

申请日：2008-03-11

Applicant: Andreas Althof ,  Stefan Fuehling ,  Wolfgang Mai ,  Knut Meyer ,  Jens Pache ,  Matthias Wiese

Inventor： Andreas Althof ,  Stefan Fuehling ,  Wolfgang Mai ,  Knut Meyer ,  Jens Pache ,  Matthias Wiese

IPC: F02M33/02

CPC classification number: F02D41/003 ,  F02D41/0045 ,  F02M25/089

Abstract: In a control method for an internal combustion engine (1) which has a fuel tank (18), a fuel vapor storage device (25) for storing the escaping fuel vapors and a controllable valve (28) for adjusting the stream of fuel vapors fed to the intake tract (4) during a period of tank ventilation, the valve (28) is controlled in such a way according to the method that the stream of fuel vapors varies during the tank ventilation period. The regeneration of the fuel vapor storage device is intended to be improved thereby.

Abstract translation: 在具有燃料箱（18）的内燃机（1）的控制方法中，具有用于储存逸出的燃料蒸气的燃料蒸气存储装置（25）和用于调节供给的燃料蒸气流的可控阀（28） 在罐通气期间到进气道（4），阀（28）按照在油箱通风期间燃料蒸汽流变化的方式进行控制。 因此，旨在提高燃料蒸气存储装置的再生。

公开(公告)号：US07893324B2

公开(公告)日：2011-02-22

申请号：US12240434

申请日：2008-09-29

Applicant: Knut Meyer

Inventor： Knut Meyer

IPC: A01H1/00 ,  C07H21/04 ,  C12N15/29 ,  C07K14/415

CPC classification number: C12N15/79 ,  A61K31/355 ,  C12N9/1007 ,  C12N15/8243

Abstract: Preparation and use of isolated nucleic acids useful in altering the oil phenotype of plants are described. Isolated nucleic acids and their encoded polypeptides are described that alter alpha- and beta-tocotrienol content in transformed seeds and oil obtained from the transformed seeds. Expression cassettes, host cells and transformed plants are described that contain the foregoing nucleic acids.

Abstract translation: 描述了可用于改变植物的油表型的分离的核酸的制备和使用。 描述了分离的核酸及其编码的多肽，其改变转化的种子中的α-和β-生育三烯酚含量，并从转化的种子获得的油。 描述了含有前述核酸的表达盒，宿主细胞和转化植物。

公开(公告)号：US20080312082A1

公开(公告)日：2008-12-18

申请号：US11927884

申请日：2007-10-30

Applicant: Anthony J. Kinney ,  Kevin L. Stecca ,  Knut Meyer

Inventor： Anthony J. Kinney ,  Kevin L. Stecca ,  Knut Meyer

IPC: A01N43/50 ,  C12N15/11 ,  A01H5/00 ,  C12Q1/68 ,  A01P13/00 ,  A01H1/02 ,  C07H21/04 ,  A01N57/18

CPC classification number: C12N15/8247 ,  A01H5/10 ,  C12N15/8274 ,  C12Q1/6895

Abstract: Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.

Abstract translation: 提供了与转基因高油酸/耐ALS抑制剂大豆植物相关的组合物和方法。 具体地说，本发明提供具有DP-305423-1事件的大豆植物，其给予至少一种ALS抑制性除草剂高油酸表型和耐受性。 携带DP-305423-1事件的大豆植物包含至少具有SEQ ID NO：8,9,14,15,20,21,83或84的多核苷酸序列的基因组/转基因接头。基因组插入位点的表征 的DP-305423-1事件提供了增强的育种效率，并且能够使用分子标记物来跟踪育种种群中的转基因插入物和其后代。 提供了用于鉴定，检测和使用大豆DP-305423-1事件的各种方法和组合物。

公开(公告)号：US20080223865A1

公开(公告)日：2008-09-18

申请号：US11994590

申请日：2006-06-27

Applicant: Andreas Althof ,  Stefan Fuehling ,  Christian Krogull ,  Knut Meyer ,  Frank Reiter

Inventor： Andreas Althof ,  Stefan Fuehling ,  Christian Krogull ,  Knut Meyer ,  Frank Reiter

IPC: B60K15/035 ,  F02M37/04

CPC classification number: F02M37/106 ,  B60K15/03504 ,  B60K15/077 ,  B60K2015/03111 ,  B60K2015/03467 ,  B60K2015/03509 ,  B60K2015/03557

Abstract: A device for delivering fuel has a fuel delivery unit placed inside a fuel tank and which consists of a swirl pot with a fuel pump situated therein for delivering fuel out via a flow line to an internal combustion engine, and with a ventilation system located inside the fuel tank and serves to withdraw fuel vapors from the fuel tank. This ventilation system consists of ventilation lines and a liquid trap for precipitating fuel. The ventilation lines are arranged with one end in the upper area of the fuel tank, and, with the other end, lead into the liquid trap. An activated charcoal filter located downstream from the liquid trap has a connection to the atmosphere. The liquid trap and the activated charcoal filter are situated next and at the height of the swirl pot. At least the liquid trap is mechanically connected to the swirl pot.

Abstract translation: 用于输送燃料的装置具有放置在燃料箱内部的燃料输送单元，该燃料输送单元由具有位于其中的燃料泵的涡流罐组成，用于经由流动管线将燃料输出到内燃机，以及位于内部的通风系统 燃料箱，用于从燃料箱中取出燃料蒸汽。 该通风系统由通风管道和用于沉淀燃料的液体捕集器组成。 通风管一端设置在燃油箱的上部区域，另一端引入液阱。 位于液阱的下游的活性炭过滤器与大气连接。 液体捕集器和活性炭过滤器位于涡流锅的下一个处和高度处。 至少液体捕集器机械地连接到涡流罐。

公开(公告)号：US07361811B2

公开(公告)日：2008-04-22

申请号：US10718311

申请日：2003-11-20

Applicant: Knut Meyer ,  Paul V. Viitanen ,  Drew E. Van Dyk

Inventor： Knut Meyer ,  Paul V. Viitanen ,  Drew E. Van Dyk

IPC: C12N15/29 ,  C12N15/82 ,  A01H5/00

CPC classification number: C12N9/88 ,  C07K2319/00 ,  C12N15/8243

Abstract: The invention relates to high-level production of pHBA in green plants using a unique expression cassette. The latter comprises a chorismate pyruvate lyase (CPL) coding sequence operably linked to a suitable promoter capable of driving protein expression in higher plants. Additionally, the CPL cassette comprises a sequence encoding a chloroplast transit peptide, its natural cleavage site, and a small portion of the transit peptide donor protein fused to the N-terminus of CPL. The chloroplast targeting sequence targets the foreign protein to the chloroplast compartment and aids in its uptake into the organelle. The cleavage site is unique to the transit peptide, and cleavage of the chimeric protein encoded by the cassette at this site releases a novel polypeptide that has full enzyme activity, comprising the mature CPL enzyme and a small portion of the transit peptide donor.

Abstract translation: 本发明涉及使用独特的表达盒在绿色植物中高水平生产pHBA。 后者包括可操作地连接到能够驱动高等植物中的蛋白质表达的合适的启动子的草酸丙酸裂解酶（CPL）编码序列。 此外，CPL盒包含编码叶绿体转运肽，其天然切割位点的序列和与CPL的N末端融合的转运肽供体蛋白的一小部分。 叶绿体靶向序列将外来蛋白质靶向叶绿体隔室，并有助于其吸收到细胞器中。 切割位点对于转运肽是独特的，并且在该位点处由盒编码的嵌合蛋白质的切割释放出具有完整酶活性的新型多肽，其包含成熟的CPL酶和少量的转运肽供体。

公开(公告)号：US20070184092A1

公开(公告)日：2007-08-09

申请号：US11656047

申请日：2007-01-22

Applicant: Knut Meyer ,  Bo Shen ,  Mitchell Tarczynski

Inventor： Knut Meyer ,  Bo Shen ,  Mitchell Tarczynski

IPC: A01H5/00 ,  A61K47/00

CPC classification number: C12N15/8247 ,  A61K9/0024 ,  A61K9/0043 ,  C12N15/8243 ,  C12N15/8271

Abstract: Compositions comprising a modulated tocol content in a plant or plant part are provided. In specific embodiments, the compositions and methods of the invention modulate tocol content by modulating the level of a polypeptide having a LEC1-type B domain in combination with modulating the level of at least one other polypeptide involved in tocol biosynthesis. Plants, plant parts, grain, seed and oil having the modulated tocol level are also provided. Methods to enhance oxidative stress tolerance of a plant or plant part, increase shelf-life, enhance the nutritional value, and improve tissue quality are also provided.

Abstract translation: 提供了包含植物或植物部分中调制的多克隆内容物的组合物。 在具体实施方案中，本发明的组合物和方法通过调节具有LEC1型B结构域的多肽的水平并结合调节参与多巴胺生物合成的至少一种其他多肽的水平来调节多克隆含量。 还提供了植物，植物部分，具有调制多酚水平的谷物，种子和油。 还提供了提高植物或植物部分的氧化应激耐受性，增加保质期，增强营养价值和改善组织质量的方法。

公开(公告)号：US20060177905A1

公开(公告)日：2006-08-10

申请号：US11403388

申请日：2006-04-13

Applicant: Paul Viitanen ,  Drew Van Dyk ,  Knut Meyer

Inventor： Paul Viitanen ,  Drew Van Dyk ,  Knut Meyer

IPC: C07H21/04 ,  C12P21/06 ,  C12N9/10

CPC classification number: C12N9/1051 ,  C07K2319/00 ,  C12N15/8243

Abstract: This invention pertains to nucleic acid fragments encoding plant glucosyltransferases, heretofore undescribed, that exhibit catalytic activity with p-hydroxybenzoic acid (pHBA) as a substrate and only attach glucose to the aromatic carboxyl group of pHBA, to form the pHBA glucose ester. These enzymes have potential applications both in vitro and in vivo, and their primary amino acid sequences can be used to identify other proteins that have similar kinetic properties.