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38 条记录 (耗时 0.031 秒)

    Method for the quantification of methylated DNA
    31.
    发明授权
    Method for the quantification of methylated DNA 有权
    甲基化DNA定量的方法

    公开(公告)号：US08703414B2

    公开(公告)日：2014-04-22

    申请号：US11989190

    申请日：2006-07-20

    申请人： Reimo Tetzner

    发明人： Reimo Tetzner

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6809 ,  C12Q1/6818 ,  C12Q2561/113 ,  C12Q2535/131 ,  C12Q2523/125

    摘要： The method according to the invention concerns in particular a method for the quantification of methylated DNA. For this purpose, the DNA to be examined is first transformed such that unmethylated cytosine is converted to uracil while 5-methylcytosine remains unchanged. Subsequently, the transformed DNA is amplified in the presence of a pair of real-time probes. For this, a probe is constructed, which is specific for the methylated or for the unmethylated state of the DNA, and a probe, which binds methylation-unspecifically to the amplificate. The ratio of the signal intensities of the probes or the CT values allows for the calculation of the degree of methylation of the examined DNA. The method according to the invention is suited particularly for the diagnosis and prognosis of cancer and other diseases associated with a change in the methylation status, as well as, prediction of adverse for side-effects of pharmaceuticals.

    摘要翻译： 根据本发明的方法特别涉及用于定量甲基化DNA的方法。 为此目的，首先将待检测的DNA转化成未甲基化胞嘧啶转化为尿嘧啶，而5-甲基胞嘧啶保持不变。 随后，在一对实时探针的存在下扩增转化的DNA。 为此，构建了针对DNA的甲基化或未甲基化状态特异的探针，以及探针，其将甲基化特异性结合于扩增子。 探针的信号强度或CT值的比值允许计算被检DNA的甲基化程度。 根据本发明的方法特别适用于与甲基化状态改变相关的癌症和其它疾病的诊断和预后，以及对药物副作用的不良预测。

    Carry-Over Protection in Enzyme-Based Dna Amplification Systems Targeting Methylation Analysis
    33.
    发明申请
    Carry-Over Protection in Enzyme-Based Dna Amplification Systems Targeting Methylation Analysis 审中-公开
    基于甲基化分析的酶基Dna扩增系统中的保护

    公开(公告)号：US20110027834A1

    公开(公告)日：2011-02-03

    申请号：US12308149

    申请日：2007-06-08

    申请人： Reimo Tetzner ,  Dimo Dietrich

    发明人： Reimo Tetzner ,  Dimo Dietrich

    IPC分类号： C12P19/34 ,  C12N9/00 ,  C07H21/04

    CPC分类号： C12Q1/6848 ,  C12Q2525/131 ,  C12Q2523/125

    摘要： The invention refers to a method for providing a decontaminated template nucleic acid for enzymatic amplification reactions suitable for DNA methylation analysis. This method is characterized by the following steps: a) incubating a nucleic acid with a chemical reagent or an enzyme-containing solution, whereby the unmethylated cytosine bases are converted into uracil bases, b) mixing the template nucleic acid from step a) with the components required for an enzyme-mediated amplification reaction, including at least two oligonucleotides, whereby at least one of said oligonucleotides comprises i) at least one sequence part that hybridizes with a sequence of the template nucleic acid to be amplified, and ii) at least one sequence part that constitutes a recognition site for a DNA cleaving enzyme that cleaves DNA downstream of said recognition site and c) adding to this mixture a DNA cleaving enzyme, which specifically binds to the at least one sequence part that is a recognition site, and d) incubating the mixture, whereby nucleic acids containing said recognition site for a DNA cleaving enzyme are degraded.

    摘要翻译： 本发明涉及一种用于提供适用于DNA甲基化分析的酶扩增反应的去污染模板核酸的方法。 该方法的特征在于以下步骤：a）将核酸与化学试剂或含酶溶液一起孵育，由此将未甲基化的胞嘧啶碱基转化成尿嘧啶碱基，b）将来自步骤a）的模板核酸与 酶介导的扩增反应所需的组分，包括至少两个寡核苷酸，其中至少一个所述寡核苷酸包含i）至少一个与待扩增的模板核酸序列杂交的序列部分，和ii）至少 构成用于切割所述识别位点下游的DNA的DNA切割酶的识别位点的一个序列部分，和c）向该混合物中加入特异性结合作为识别位点的至少一个序列部分的DNA切割酶，以及 d）孵育混合物，由此含有用于DNA切割酶的所述识别位点的核酸被降解。

    Method for investigating cytosine methylation in dna by means of dna repair enzymes
    37.
    发明申请
    Method for investigating cytosine methylation in dna by means of dna repair enzymes 失效
    通过dna修复酶研究dna中胞嘧啶甲基化的方法

    公开(公告)号：US20070160995A1

    公开(公告)日：2007-07-12

    申请号：US10585682

    申请日：2005-01-10

    申请人： Reimo Tetzner ,  Kurt Berlin ,  Jurgen Distler

    发明人： Reimo Tetzner ,  Kurt Berlin ,  Jurgen Distler

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  C12Q2537/113 ,  C12Q2523/125 ,  C12Q2521/514

    摘要： The following invention concerns a method for investigating cytosine methylation by means of DNA repair enzymes. Here, the DNA is first converted so that unmethylated cytosines are converted to uracil, while 5-methylcytosine remains unchanged. Then the DNA is hybridized to oligonucleotides, whereby hybrids will be formed with or without erroneous base pairings, in each case depending on the methylation status of the DNA. Following this, the erroneously paired hybrids will be cleaved by repair enzymes. Then the methylation status of the DNA can be determined in different ways. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer disorders and other diseases associated with a change of the methylation status as well as for predicting undesired drug effects.

    摘要翻译： 以下发明涉及通过DNA修复酶研究胞嘧啶甲基化的方法。 这里首先转化DNA，使未甲基化的胞嘧啶转化为尿嘧啶，而5-甲基胞嘧啶保持不变。 然后将DNA与寡核苷酸杂交，由此可以形成带有或没有错误碱基配对的杂交体，在每种情况下都取决于DNA的甲基化状态。 之后，错误配对的杂交体将被修复酶切割。 那么DNA的甲基化状态可以通过不同的方法来确定。 根据本发明的方法特别适用于与甲基化状态改变相关的癌症和其它疾病的诊断和预后以及预测不期望的药物作用。

    Method for the detection of cytosine methylations in dna with the aid of scorpion
    38.
    发明申请
    Method for the detection of cytosine methylations in dna with the aid of scorpion 有权
    借助蝎子检测dna中胞嘧啶甲基化的方法

    公开(公告)号：US20060194208A1

    公开(公告)日：2006-08-31

    申请号：US10568300

    申请日：2004-08-13

    申请人： Reimo Tetzner ,  Jürgen Distler

    发明人： Reimo Tetzner ,  Jürgen Distler

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/154

    摘要： The invention relates to a method for analyzing cytosine methylations in DNA sequences, according to which non-methylated cytosines are first converted into uracil while 5-methylcytosine remains unmodified. The DNA is then amplified by means of a polymerase and at least one primer whose 5 end is connected to a probe via a linker. The probe is intramolecularly hybridized onto the amplified products in accordance with the methylation state of the DNA, hybridization being detectable via different detection systems. The inventive method is particularly suitable for diagnosing and predicting cancer diseases and other diseases associated with a modification of the methylation state as well as for predicting undesired effects of medicaments.

    摘要翻译： 本发明涉及一种用于分析DNA序列中胞嘧啶甲基化的方法，根据该方法，非甲基化胞嘧啶首先转化成尿嘧啶，而5-甲基胞嘧啶保持未改变。 然后通过聚合酶和至少一个引物将DNA扩增，其末端通过接头与探针连接。 根据DNA的甲基化状态，探针在扩增产物上分子内杂交，通过不同的检测系统检测杂交。 本发明的方法特别适用于诊断和预测与改变甲基化状态相关的癌症疾病和其它疾病以及用于预测不期望的药物作用。