公开(公告)号：US06265204B1

公开(公告)日：2001-07-24

申请号：US08785668

申请日：1997-01-17

Applicant: Michael Ward ,  Scott D. Power

Inventor： Michael Ward ,  Scott D. Power

IPC: C12N115

CPC classification number: C12N15/80 ,  C07K2319/036 ,  C07K2319/20 ,  C07K2319/42 ,  C12N15/625

Abstract: The invention includes novel fusion nucleic acids encoding fusion polypeptides which when expressed in a filamentous fungus result in the expression of fusion polypeptides. The fusion nucleic acids comprise four nucleic acids which encode a fusion polypeptide comprising first, second, third and fourth amino acid sequences. The first nucleic acid encodes a signal polypeptide functional as a secretory sequence in a first filamentous fungus. The second nucleic acid encodes a secreted polypeptide or functional portion thereof which is normally secreted from the same filamentous fungus or a second filamentous fungus. The third nucleic acid encodes a cleavable linker while the fourth nucleic acid comprises at least two nucleic acids encoding desired polypeptides.

Abstract translation: 本发明包括编码融合多肽的新型融合核酸，其在丝状真菌中表达时导致融合多肽的表达。 融合核酸包含编码包含第一，第二，第三和第四氨基酸序列的融合多肽的四个核酸。 第一核酸编码在第一丝状真菌中作为分泌序列起作用的信号多肽。 第二核酸编码通常由相同的丝状真菌或第二丝状真菌分泌的分泌的多肽或其功能部分。 第三核酸编码可切割的接头，而第四核酸包含编码所需多肽的至少两个核酸。

公开(公告)号：US5824532A

公开(公告)日：1998-10-20

申请号：US468220

申请日：1995-06-06

Applicant: Christopher C. Barnett ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

Inventor： Christopher C. Barnett ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

IPC: C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N5/10 ,  C12N9/26 ,  C12N9/28 ,  C12N15/56 ,  C12N15/75 ,  C12P19/14 ,  C12N15/63

CPC classification number: C12N9/2417 ,  C11D3/386 ,  C12N15/75 ,  C12P19/14

Abstract: Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

Abstract translation: 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。

公开(公告)号：US5411873A

公开(公告)日：1995-05-02

申请号：US928697

申请日：1992-08-11

Applicant: Robin M. Adams ,  Scott D. Power ,  David B. Powers ,  James A. Wells ,  Daniel G. Yansura

Inventor： Robin M. Adams ,  Scott D. Power ,  David B. Powers ,  James A. Wells ,  Daniel G. Yansura

IPC: C11D3/386 ,  C12N9/54 ,  C12N15/10 ,  C12N15/52 ,  C12N15/75 ,  C12P21/02

CPC classification number: C12N15/52 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54 ,  C12R1/07

Abstract: Processes for producing various heterologous polypeptides which when expressed are either incorrectly processed and hence asssociated with the surface of the host cell or are not processed to mature form. More specifically, processes for the production of heterologous non-human carbonyl hydrolases expressed either in host cells incapable of producing enzymatically active endoprotease or host cells deficient in enzymatically active extracellular endoprotease are disclosed. Such non-human carbonyl hydrolases generally are incapable of autoproteolytic maturation and become associated with the surface of expression hosts which are deficient in enzymatically active extracellular endoprotease. Processes for preparing non-human carbonyl hydrolase and heterologous polypeptides which are expressed as part of a fusion polypeptide are also disclosed, as well as non-human carbonyl hydrolases which are substantially free of the host cell membrane with which they are normally associated.

Abstract translation: 用于产生各种异源多肽的方法，当被表达时，它们被错误地加工并因此与宿主细胞的表面相结合或者不被加工成成熟形式。 更具体地，公开了在不能产生酶促活性内切蛋白酶的宿主细胞中表达的异源非人羰基水解酶的生产方法或在酶活性细胞外内蛋白酶中缺乏的宿主细胞。 这种非人羰基水解酶通常不能自身蛋白水解成熟，并且与酶活性细胞外内蛋白酶缺陷的表达宿主的表面相关联。 还公开了制备非人羰基水解酶和作为融合多肽的一部分表达的异源多肽的方法，以及基本上不含与它们通常结合的宿主细胞膜的非人羰基水解酶。

公开(公告)号：US08512982B2

公开(公告)日：2013-08-20

申请号：US12743187

申请日：2008-12-19

Applicant: Marguerite A. Cervin ,  Eugenio Ferrari ,  Scott D. Power

Inventor： Marguerite A. Cervin ,  Eugenio Ferrari ,  Scott D. Power

IPC: C12P21/02 ,  C12N15/63 ,  C07H21/04

CPC classification number: C12N15/63 ,  C07K14/32 ,  C12N15/75

Abstract: The present invention relates to cells that have been genetically manipulated to have an altered capacity to express and/or produce proteins of interest. In particular, the present invention relates to modified host cells of Gram-positive microorganisms, such as Bacillus species that are capable of overexpressing ymaH. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode YmaH, and the modified host cells comprising them. In particular, the present invention relates to compositions and methods of overexpressing YmaH for enhancing the expression and production of proteins of interest (e.g., proteases) in Bacillus species.

Abstract translation: 本发明涉及已被遗传操作以具有改变的表达和/或产生感兴趣的蛋白质的能力的细胞。 特别地，本发明涉及革兰氏阳性微生物的修饰宿主细胞，例如能够过表达ymaH的芽孢杆菌属物种。 本发明包括含有编码YmaH的多核苷酸序列的多核苷酸构建体和表达载体，以及包含它们的修饰的宿主细胞。 特别地，本发明涉及过表达YmaH用于增强芽孢杆菌属物种中目的蛋白质（例如蛋白酶）的表达和产生的组合物和方法。

公开(公告)号：US08153412B2

公开(公告)日：2012-04-10

申请号：US12264006

申请日：2008-11-03

Applicant: Claudine Y. Chang ,  Clement Choy ,  Melodie Estabrook ,  Mansi Goyal ,  Thomas P. Graycar ,  Victoria E. Huang ,  Brian E. Jones ,  Marc Kolkman ,  Karsten M. Kragh ,  Chris Leeflang ,  Scott D. Power ,  Sandra W. Ramer ,  Andrew Shaw ,  Casper Vroemen ,  Walter Weyler

Inventor： Claudine Y. Chang ,  Clement Choy ,  Melodie Estabrook ,  Mansi Goyal ,  Thomas P. Graycar ,  Victoria E. Huang ,  Brian E. Jones ,  Marc Kolkman ,  Karsten M. Kragh ,  Chris Leeflang ,  Scott D. Power ,  Sandra W. Ramer ,  Andrew Shaw ,  Casper Vroemen ,  Walter Weyler

IPC: C12N9/28 ,  C12N9/26 ,  C12N9/24 ,  C12N1/20 ,  C12N15/00 ,  A23C17/00 ,  A23L1/28 ,  C11D3/02 ,  C12D3/00 ,  C12D3/386 ,  C07H21/04 ,  C07K1/00

CPC classification number: C11D3/386 ,  C11D3/38681 ,  C12N9/2417

Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.

Abstract translation: 芽孢杆菌的变种 TS-23菌株α-淀粉酶表现出改善的酶性能，包括增加其稳定性，降低钙依赖性，提高洗涤/清洁性能和烘烤能力。 包含这些变体的组合物可用于淀粉加工，淀粉液化，粉化，淀粉糖化，清洗，洗衣，纺织品退浆，烘烤和生物膜除去的方法。

公开(公告)号：US20110263517A1

公开(公告)日：2011-10-27

申请号：US12958139

申请日：2010-12-01

Applicant: Scott D. Power ,  Fiona A. Harding

Inventor： Scott D. Power ,  Fiona A. Harding

IPC: A61K38/02 ,  C07K14/52 ,  C07K14/715 ,  C12N1/00 ,  C07K1/107 ,  C07H21/00 ,  C12N15/63 ,  C40B30/06 ,  C07K7/08

CPC classification number: G01N33/6878 ,  A61K38/00 ,  C07K14/505 ,  C07K14/524 ,  C07K14/565 ,  C07K14/7151 ,  G01N33/505 ,  G01N33/56972 ,  G01N33/6863

Abstract: The present invention provides methods for the identification of CD4+ T-cell epitopes in the sequences of various proteins, namely, human cytokines and cytokine receptors, as well as the production of peptides which when incorporated into the protein sequence, are no longer capable of initiating the CD4+ T-cell response. In some embodiments, the present invention provides means and compositions suitable for reducing the immunogenicity of cytokines and cytokines receptors such as interferon-β, soluble tumor necrosis factor receptor-1, erythoropoietin, and thrombopoietin.

Abstract translation: 本发明提供了鉴定各种蛋白质序列中的CD4 + T细胞表位的方法，即人类细胞因子和细胞因子受体，以及当其并入蛋白质序列时不再能起始的肽的产生 CD4 + T细胞应答。 在一些实施方案中，本发明提供适于降低细胞因子和细胞因子受体如干扰素-α，可溶性肿瘤坏死因子受体-1，促红细胞生成素和血小板生成素的免疫原性的方法和组合物。

公开(公告)号：US20110230405A1

公开(公告)日：2011-09-22

申请号：US12403272

申请日：2009-03-12

Applicant: Anthony G. Day ,  David A. Estell ,  Christopher J. Murray ,  Scott D. Power

Inventor： Anthony G. Day ,  David A. Estell ,  Christopher J. Murray ,  Scott D. Power

IPC: A61K38/56 ,  A61P9/00

CPC classification number: C07K14/81 ,  A61K38/00 ,  C07K7/06 ,  C07K14/811 ,  C07K2319/00 ,  C12N15/62

Abstract: The present invention provides peptides and supported peptides for treating proliferative diseases. In particularly preferred embodiments, the present invention provides peptides and supported peptides for treating diseases of the skin, such as rosacea. In some particularly preferred embodiments, the supported peptides of the present invention are anti-VEGF peptides. In alternative particularly preferred embodiments, the anti-VEGF peptides are expressed on a scaffold protein. In some most preferred embodiments, the scaffold protein is BBI.

Abstract translation: 本发明提供用于治疗增殖性疾病的肽和负载肽。 在特别优选的实施方案中，本发明提供用于治疗皮肤疾病的肽和负载肽，例如红斑痤疮。 在一些特别优选的实施方案中，本发明的负载型肽是抗VEGF肽。 在替代的特别优选的实施方案中，抗VEGF肽在支架蛋白上表达。 在一些最优选的实施方案中，支架蛋白是BBI。

公开(公告)号：US20110117598A1

公开(公告)日：2011-05-19

申请号：US12743187

申请日：2008-12-19

Applicant: Marguerite A. Cervin ,  Eugenio Ferrari ,  Scott D. Power

Inventor： Marguerite A. Cervin ,  Eugenio Ferrari ,  Scott D. Power

IPC: C12P21/02 ,  C07H21/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N15/75 ,  C12N9/00

CPC classification number: C12N15/63 ,  C07K14/32 ,  C12N15/75

Abstract: The present invention relates to cells that have been genetically manipulated to have an altered capacity to express and/or produce proteins of interest. In particular, the present invention relates to modified host cells of Gram-positive microorganisms, such as Bacillus species that are capable of overexpressing ymaH. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode YmaH, and the modified host cells comprising them. In particular, the present invention relates to compositions and methods of overexpressing YmaH for enhancing the expression and production of proteins of interest (e.g., proteases) in Bacillus species.

Abstract translation: 本发明涉及已被遗传操作以具有改变的表达和/或产生感兴趣的蛋白质的能力的细胞。 特别地，本发明涉及革兰氏阳性微生物的修饰宿主细胞，例如能够过表达ymaH的芽孢杆菌属物种。 本发明包括含有编码YmaH的多核苷酸序列的多核苷酸构建体和表达载体，以及包含它们的修饰的宿主细胞。 特别地，本发明涉及过表达YmaH用于增强芽孢杆菌属物种中目的蛋白质（例如蛋白酶）的表达和产生的组合物和方法。

公开(公告)号：US07888472B2

公开(公告)日：2011-02-15

申请号：US12408472

申请日：2009-03-20

Applicant: Alessandro Sette ,  Robert Chesnut ,  Mark J. Newman ,  Brian D. Livingston ,  Lilia Maria Babe ,  Yiyou Chen ,  Lawrence M. Deyoung ,  Manley T. F. Huang ,  Scott D. Power

Inventor： Alessandro Sette ,  Robert Chesnut ,  Mark J. Newman ,  Brian D. Livingston ,  Lilia Maria Babe ,  Yiyou Chen ,  Lawrence M. Deyoung ,  Manley T. F. Huang ,  Scott D. Power

IPC: C07K14/00 ,  A61K39/00

CPC classification number: A61K39/292 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55555 ,  A61K2039/6031 ,  A61K2039/605 ,  A61K2039/645 ,  C07K14/005 ,  C12N2730/10122 ,  C12N2730/10134

Abstract: The invention relates to the field of biology. In particular, it relates to multi-epitope nucleic acid and peptide vaccines and methods of designing such vaccines to provide increased immunogenicity.

Abstract translation: 本发明涉及生物学领域。 特别地，其涉及多表位核酸和肽疫苗以及设计这种疫苗以提供增加的免疫原性的方法。

公开(公告)号：US20100015686A1

公开(公告)日：2010-01-21

申请号：US12479427

申请日：2009-06-05

Applicant: Luis G. Cascao-Pereira ,  William A. Cuevas ,  David A. Estell ,  Sang-Kyu Lee ,  Scott D. Power ,  Sandra W. Ramer ,  Amr Toppozada ,  Louise Wallace

Inventor： Luis G. Cascao-Pereira ,  William A. Cuevas ,  David A. Estell ,  Sang-Kyu Lee ,  Scott D. Power ,  Sandra W. Ramer ,  Amr Toppozada ,  Louise Wallace

IPC: C12N9/28

CPC classification number: C12N9/2417 ,  A21D8/042 ,  C12N9/2408 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  G01N11/00 ,  Y02E50/17

Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.

Abstract translation: 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。