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    Process for producing heterologous polypeptides
    1.
    发明授权
    Process for producing heterologous polypeptides 失效
    用于产生异源多肽的方法

    公开(公告)号：US5411873A

    公开(公告)日：1995-05-02

    申请号：US928697

    申请日：1992-08-11

    申请人： Robin M. Adams ,  Scott D. Power ,  David B. Powers ,  James A. Wells ,  Daniel G. Yansura

    发明人： Robin M. Adams ,  Scott D. Power ,  David B. Powers ,  James A. Wells ,  Daniel G. Yansura

    IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N15/10 ,  C12N15/52 ,  C12N15/75 ,  C12P21/02

    CPC分类号： C12N15/52 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54 ,  C12R1/07

    摘要： Processes for producing various heterologous polypeptides which when expressed are either incorrectly processed and hence asssociated with the surface of the host cell or are not processed to mature form. More specifically, processes for the production of heterologous non-human carbonyl hydrolases expressed either in host cells incapable of producing enzymatically active endoprotease or host cells deficient in enzymatically active extracellular endoprotease are disclosed. Such non-human carbonyl hydrolases generally are incapable of autoproteolytic maturation and become associated with the surface of expression hosts which are deficient in enzymatically active extracellular endoprotease. Processes for preparing non-human carbonyl hydrolase and heterologous polypeptides which are expressed as part of a fusion polypeptide are also disclosed, as well as non-human carbonyl hydrolases which are substantially free of the host cell membrane with which they are normally associated.

    摘要翻译： 用于产生各种异源多肽的方法，当被表达时，它们被错误地加工并因此与宿主细胞的表面相结合或者不被加工成成熟形式。 更具体地，公开了在不能产生酶促活性内切蛋白酶的宿主细胞中表达的异源非人羰基水解酶的生产方法或在酶活性细胞外内蛋白酶中缺乏的宿主细胞。 这种非人羰基水解酶通常不能自身蛋白水解成熟，并且与酶活性细胞外内蛋白酶缺陷的表达宿主的表面相关联。 还公开了制备非人羰基水解酶和作为融合多肽的一部分表达的异源多肽的方法，以及基本上不含与它们通常结合的宿主细胞膜的非人羰基水解酶。

    Specific N-terminal labeling of peptides and proteins in complex mixtures
    4.
    发明授权
    Specific N-terminal labeling of peptides and proteins in complex mixtures 有权
    复杂混合物中肽和蛋白质的特异性N末端标记

    公开(公告)号：US08679771B2

    公开(公告)日：2014-03-25

    申请号：US12524557

    申请日：2008-01-24

    申请人： James A. Wells ,  Sami Mahrus

    发明人： James A. Wells ,  Sami Mahrus

    IPC分类号： G01N33/535

    CPC分类号： G01N33/6803 ,  C12Q1/34

    摘要： This invention provides general methods for selective labeling of proteins on their N-termini with synthetic peptides. The methods of this invention can be applied to the global proteomic profiling of complex mixtures of proteins and polypeptides.

    摘要翻译： 本发明提供了用合成肽在其N-末端上选择性标记蛋白质的一般方法。 本发明的方法可以应用于蛋白质和多肽的复合物混合物的全局蛋白质组学分析。

    Peptide variants of protein A
    7.
    发明授权
    Peptide variants of protein A 失效
    蛋白A的肽变体

    公开(公告)号：US06197927B1

    公开(公告)日：2001-03-06

    申请号：US09036549

    申请日：1998-03-06

    申请人： Andrew C. Braisted ,  Melissa A. Starovasnik ,  James A. Wells

    发明人： Andrew C. Braisted ,  Melissa A. Starovasnik ,  James A. Wells

    IPC分类号： C07K512

    CPC分类号： C07K14/31 ,  C07K2319/00 ,  Y10S436/828

    摘要： Z domain variants of staphylococcal protein A have significantly reduced size but possess IgG-binding affinity equivalent to the wild type Z domain. These Z domain variants are suitable for use in affinity chromatography purification of proteins and in the treatment of staphylococcic diseases.

    摘要翻译： 葡萄球菌蛋白A的Z结构域变体具有显着减小的大小，但具有与野生型Z结构域相当的IgG结合亲和力。 这些Z结构域变体适用于蛋白质的亲和层析纯化和葡萄球菌性疾病的治疗。

    Method for identifying active domains and amino acid residues in polypeptides and hormone variants
    9.
    发明授权
    Method for identifying active domains and amino acid residues in polypeptides and hormone variants 失效
    用于鉴定多肽和激素变体中活性结构域和氨基酸残基的方法

    公开(公告)号：US06013478A

    公开(公告)日：2000-01-11

    申请号：US104036

    申请日：1998-06-24

    申请人： James A. Wells ,  Brian C. Cunningham

    发明人： James A. Wells ,  Brian C. Cunningham

    IPC分类号： A61K38/24 ,  C07K1/00 ,  C07K14/575 ,  C07K14/61 ,  C07K16/00 ,  C12N1/21 ,  C12N15/00 ,  C12N15/16 ,  C12N15/18 ,  C12N15/63 ,  C12P21/06 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G06F19/00

    CPC分类号： C07K14/575 ,  C12N15/1058 ,  Y10S530/806 ,  Y10S530/808

    摘要： The invention provides methods for the systematic analysis of the structure and function of polypeptides by identifying active domains which influence the activity of the polypeptide with a target substance. Such active domains are determined by substituting selected amino acid segments of the polypeptide with an analogous polypeptide segment from an analog to the polypeptide. The analog has a different activity with the target substance as compared to the parent polypeptide. The activities of the segment-substituted polypeptides are compared to the same activity for the parent polypeptide for the target. A comparison of such activities provides an indication of the location of the active domain in the parent polypeptide. The invention also provides methods for identifying the active amino acid residues within the active domain of the parent polypeptide. The method comprises substituting a scanning amino acid for one of the amino acid residues within the active domain of the parent polypeptide and assaying the residue-substituted polypeptide so formed with a target substance. The invention further provides polypeptide variants comprising segment-substituted and residue-substituted growth hormones, prolactins and placental lactogens.

    摘要翻译： 本发明提供了通过鉴定影响多肽与靶物质的活性的活性结构域来系统分析多肽的结构和功能的方法。 通过用类似物与多肽的类似多肽片段取代多肽的选定氨基酸片段来确定此类活性结构域。 与亲本多肽相比，类似物与目标物质具有不同的活性。 将段取代的多肽的活性与目标的亲本多肽的相同活性进行比较。 这些活性的比较提供了活性结构域在亲本多肽中的位置的指示。 本发明还提供鉴定亲本多肽活性结构域内活性氨基酸残基的方法。 该方法包括用扫描氨基酸取代母体多肽的活性结构域内的氨基酸残基之一，并测定与靶物质形成的残基取代的多肽。 本发明还提供了包含片段取代的和残基取代的生长激素，催乳素和胎盘乳糖的多肽变体。