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    Method and apparatus for nucleic acid sequencing and identification
    31.
    发明授权
    Method and apparatus for nucleic acid sequencing and identification 有权
    用于核酸测序和鉴定的方法和装置

    公开(公告)号：US07005264B2

    公开(公告)日：2006-02-28

    申请号：US10153125

    申请日：2002-05-20

    Applicant: Xing Su ,  Andrew A. Berlin

    Inventor： Xing Su ,  Andrew A. Berlin

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: G01N33/48721 ,  B01L3/5027 ,  B82Y30/00 ,  C12Q1/6869 ,  C12Q2565/631 ,  C12Q2563/155 ,  C12Q2527/113

    Abstract: The methods and apparatus 100 disclosed herein are of use for sequencing and/or identifying nucleic acids 230, 310. Nucleic acids 230, 310 containing labeled nucleotides 235, 245, 315 may be synthesized and passed through nanopores 255, 310. Detectors 257, 345 operably coupled to the nanopores 255, 310 may detect the labeled nucleotides 235, 245, 315. By determining the time intervals at which labeled nucleotides 235, 245, 315 are detected, distance maps 140 for each type of labeled nucleotide 235, 245, 315 may be compiled. The distance maps 140 in turn may be used to sequence 150 and/or identify 160 the nucleic acid 230, 310. In different embodiments of the invention, luminescent nucleotides 235, 245 or nanoparticles 315 may be detected using photodetectors 257 or electrical detectors 310. Apparatus 100 and sub-devices 200, 300 of use for nucleic acid 230, 310 sequencing 150 and/or identification 160 are also disclosed herein.

    Abstract translation: 本文公开的方法和装置100可用于测序和/或鉴定核酸230,310。 可以合成含有标记核苷酸235,245,315的核酸230,310并通过纳米孔255,310。 可操作地偶联到纳米孔255,310的检测器257,345可以检测标记的核苷酸235,245,315。 通过确定检测到标记核苷酸235,245,315的时间间隔，可以编译每种类型的标记核苷酸235,245,315的距离图140。 距离图140依次可用于对核酸230,310进行序列150和/或识别160。 在本发明的不同实施方案中，可以使用光电检测器257或电检测器310检测发光核苷酸235,245或纳米颗粒315。 本文还公开了用于核酸230,310测序150和/或识别160的装置100和子装置200,300。

    Methods to increase nucleotide signals by Raman scattering
    32.
    发明申请
    Methods to increase nucleotide signals by Raman scattering 审中-公开
    通过拉曼散射增加核苷酸信号的方法

    公开(公告)号：US20060029969A1

    公开(公告)日：2006-02-09

    申请号：US11235796

    申请日：2005-09-26

    Applicant: Xing Su ,  Selena Chan ,  Andrew Berlin ,  Tae-Woong Koo ,  Narayan Sundararajan ,  Mineo Yamakawa

    Inventor： Xing Su ,  Selena Chan ,  Andrew Berlin ,  Tae-Woong Koo ,  Narayan Sundararajan ,  Mineo Yamakawa

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6872 ,  C12Q2565/632 ,  C12Q2563/155 ,  C12Q2521/319

    Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13. Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130, which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERFS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing.

    Abstract translation: 本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。 在本发明的某些实施方案中，核苷酸在掺入核酸13之前共价连接到拉曼标记上。 标记核酸13的核酸外切酶15处理导致通过拉曼光谱法检测的标记核苷酸16,130的释放。 在本发明的替代实施方案中，通过核酸外切酶15处理从核酸13释放的核苷酸16,130与银或金纳米颗粒140共价交联并通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERFS ）和/或相干的反斯托克斯拉曼光谱（CARS）。 本发明的其它实施方案涉及用于核酸测序的装置10,100,210。

    Methods and device for analyte characterization
    33.
    发明申请
    Methods and device for analyte characterization 有权
    用于分析物表征的方法和装置

    公开(公告)号：US20050282229A1

    公开(公告)日：2005-12-22

    申请号：US10697682

    申请日：2003-10-29

    Applicant: Xing Su ,  Andrew Berlin

    Inventor： Xing Su ,  Andrew Berlin

    IPC: G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  G01N33/68 ,  G06F19/00

    CPC classification number: G01N33/48721 ,  G01N33/582 ,  G01N33/6803 ,  G01N33/6818

    Abstract: The methods and apparatus, disclosed herein are of use for sequencing and/or identifying proteins, polypeptides and/or peptides. Proteins containing labeled amino acid residues may be synthesized and passed through nanopores. A detector operably coupled to a nanopore may detect labeled amino acid residues as they pass through the nanopore. Distance maps for each type of labeled amino acid residue may be compiled. The distance maps may be used to sequence and/or identify the protein. Apparatus of use for protein sequencing and/or identification is also disclosed herein. In alternative methods, other types of analytes may be analyzed by the same techniques.

    Abstract translation: 本文公开的方法和装置可用于测序和/或鉴定蛋白质，多肽和/或肽。 含有标记氨基酸残基的蛋白质可以合成并通过纳米孔。 可操作地耦合到纳米孔的检测器可以在通过纳米孔时检测标记的氨基酸残基。 可以编制每种类型的标记氨基酸残基的距离图。 距离图可以用于序列和/或鉴定蛋白质。 本文还公开了用于蛋白质测序和/或鉴定的装置。 在替代方法中，可以通过相同的技术分析其它类型的分析物。

    Methods for using raman spectroscopy to obtain a protein profile of a biological sample
    36.
    发明申请
    Methods for using raman spectroscopy to obtain a protein profile of a biological sample 审中-公开
    使用拉曼光谱法获得生物样品的蛋白质谱的方法

    公开(公告)号：US20050148098A1

    公开(公告)日：2005-07-07

    申请号：US10749528

    申请日：2003-12-30

    Applicant: Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Tae-Woong Koo ,  Selena Chan ,  Andrew Berlin ,  Narayanan Sundararajan

    Inventor： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Tae-Woong Koo ,  Selena Chan ,  Andrew Berlin ,  Narayanan Sundararajan

    IPC: G01N21/65 ,  G01N33/58 ,  G01N33/68 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50 ,  G01N33/543 ,  G01N33/553

    CPC classification number: G01N21/658 ,  G01N33/6803 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

    Abstract: The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.

    Abstract translation: 本发明提供了用于分析生物样品的蛋白质含量的方法，例如获得由特定个体提供的样品的蛋白质谱。 样品中的蛋白质和蛋白质片段基于化学和/或物理性质分离，并在固体基质上或流动液体流中的离散位置保持分离状态。 然后检测拉曼光谱，由离散位置处的分离的蛋白质或片段产生，使得离散位置的光谱提供关于在离散位置处的一种或多种特定蛋白质或片段的结构或身份的信息。 离散位置处的蛋白质或片段可以用金属（例如金或银）涂覆，和/或分离的蛋白质可与化学增强剂接触以提供SERS光谱。 还提供了用于实施本发明的方法和试剂盒。

    Method for isolating nucleic acids
    37.
    发明授权
    Method for isolating nucleic acids 失效
    分离核酸的方法

    公开(公告)号：US5804684A

    公开(公告)日：1998-09-08

    申请号：US519039

    申请日：1995-08-24

    Applicant: Xing Su

    Inventor： Xing Su

    IPC: B01L3/00 ,  C12N15/10 ,  C07H21/00

    CPC classification number: B01L3/502753 ,  C12N15/1006 ,  C12N15/1013 ,  B01L2200/0631 ,  B01L2300/069 ,  B01L2400/0406

    Abstract: The invention features a method of isolating nucleic acid in a substantially purified form, including the steps of: a) contacting a biological sample which contains aggregated nucleic acid with a matrix comprising a solid hydrophilic organic polymer without an effective positive charge under conditions which permit the nucleic acid to bind to the matrix; and b) recovering nucleic acid from the matrix.

    Abstract translation: 本发明的特征在于以基本纯化的形式分离核酸的方法，包括以下步骤：a）使含有聚集核酸的生物样品与包含固体亲水性有机聚合物的基质在没有有效正电荷的条件下接触，条件允许 核酸结合基质; 和b）从基质中回收核酸。

    Matched order fulfillment with linear optimization
    38.
    发明授权
    Matched order fulfillment with linear optimization 有权

    公开(公告)号：US10430878B2

    公开(公告)日：2019-10-01

    申请号：US13617845

    申请日：2012-09-14

    Applicant: Richard Co ,  John Nyhoff ,  Xing Su ,  Tuen Tuen Wang ,  John Labuszewski

    Inventor： Richard Co ,  John Nyhoff ,  Xing Su ,  Tuen Tuen Wang ,  John Labuszewski

    IPC: G06Q40/00 ,  G06Q40/04

    Abstract: A method for matching compound orders from a group of market participants includes receiving, via a communication network, compound order data, the compound order data specifying a maximum amount of a financial instrument of a plurality of financial instruments to be bought or sold by each market participant, accessing a memory in which price data is stored, the price data indicating a current price of each financial instrument, implementing, with a processor, a linear solver to maximize fulfillment of the compound orders via order matching for execution at the current prices in accordance with the maximum amounts specified in the compound order data and in accordance with a maximum net risk exposure level for each market participant arising from the fulfillment of the compound orders, and transmitting trade data indicative of the order matching for execution of trades among the market participants at the current prices.

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