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    Chemically induced optical signals and DNA sequencing
    1.
    发明授权
    Chemically induced optical signals and DNA sequencing 有权

    公开(公告)号：US09671558B2

    公开(公告)日：2017-06-06

    申请号：US12459309

    申请日：2009-06-30

    Applicant: Xing Su ,  Liming Wang ,  Jianquan Liu ,  Kai Wu

    Inventor： Xing Su ,  Liming Wang ,  Jianquan Liu ,  Kai Wu

    IPC: C12Q1/68 ,  G02B6/122

    CPC classification number: G02B6/1221 ,  C12Q1/6874 ,  C12Q2565/607 ,  C12Q2563/107 ,  C12Q2527/125

    Abstract: Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and optically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced.

    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
    3.
    发明授权
    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
    在固定流体芯片中的酶信号产生和结合复合物的检测

    公开(公告)号：US08664008B2

    公开(公告)日：2014-03-04

    申请号：US13543488

    申请日：2012-07-06

    Applicant: David J. Liu ,  Kai Wu ,  Xing Su

    Inventor： David J. Liu ,  Kai Wu ,  Xing Su

    IPC: G01N33/553 ,  C12Q1/00

    CPC classification number: G01N21/658 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2400/043 ,  B01L2400/086 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/255 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/76 ,  G01N33/54333 ,  G01N33/587

    Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

    Abstract translation: 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
    4.
    发明授权
    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
    在固定流体芯片中的酶信号产生和结合复合物的检测

    公开(公告)号：US08409877B2

    公开(公告)日：2013-04-02

    申请号：US11647565

    申请日：2006-12-29

    Applicant: David J. Liu ,  Kai Wu ,  Xing Su

    Inventor： David J. Liu ,  Kai Wu ,  Xing Su

    IPC: G01N33/553 ,  C12Q1/00

    CPC classification number: G01N21/658 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2400/043 ,  B01L2400/086 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/255 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/76 ,  G01N33/54333 ,  G01N33/587

    Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

    Abstract translation: 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

    Solid-phase chelators and electronic biosensors
    5.
    发明申请
    Solid-phase chelators and electronic biosensors 有权
    固相螯合剂和电子生物传感器

    公开(公告)号：US20110159481A1

    公开(公告)日：2011-06-30

    申请号：US12655459

    申请日：2009-12-30

    Applicant: David J. Liu ,  Xing Su ,  Kai Wu

    Inventor： David J. Liu ,  Xing Su ,  Kai Wu

    IPC: C12Q1/68 ,  G01N33/50 ,  G01N27/26

    CPC classification number: C12Q1/48 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N2333/9125 ,  C12Q2565/607 ,  C12Q2565/507 ,  C12Q2565/301

    Abstract: Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and electronically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced. Additionally, devices and methods are provided for detecting biomolecules through the detection of pyrophosphate ions.

    Abstract translation: 介绍了核酸测序方法。 通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。 在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸， 切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。 焦磷酸根离子被底物连接的螯合剂捕获并电子检测以确定待测序的DNA分子中下一个互补核酸的同一性。 另外，提供了通过检测焦磷酸根离子来检测生物分子的装置和方法。

    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
    6.
    发明申请
    Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
    在固定流体芯片中的酶信号产生和结合复合物的检测

    公开(公告)号：US20080160630A1

    公开(公告)日：2008-07-03

    申请号：US11647565

    申请日：2006-12-29

    Applicant: David J. Liu ,  Kai Wu ,  Xing Su

    Inventor： David J. Liu ,  Kai Wu ,  Xing Su

    IPC: G01N21/00 ,  G01N1/00

    CPC classification number: G01N21/658 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2400/043 ,  B01L2400/086 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/255 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/76 ,  G01N33/54333 ,  G01N33/587

    Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

    Abstract translation: 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性粒子和/或信号粒子。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

    ENZYMATIC SIGNAL GENERATION AND DETECTION OF BINDING COMPLEXES IN STATIONARY FLUIDIC CHIP
    7.
    发明申请
    ENZYMATIC SIGNAL GENERATION AND DETECTION OF BINDING COMPLEXES IN STATIONARY FLUIDIC CHIP 有权
    固定信号生成和检测固定液流芯片中的结合复合物

    公开(公告)号：US20120322683A1

    公开(公告)日：2012-12-20

    申请号：US13543488

    申请日：2012-07-06

    Applicant: David J. Liu ,  Kai Wu ,  Xing Su

    Inventor： David J. Liu ,  Kai Wu ,  Xing Su

    IPC: G01N33/558 ,  G01N33/53 ,  C40B30/04 ,  G01N33/573 ,  G01N21/78 ,  G01N27/26 ,  G01N21/00 ,  G01N21/76 ,  C12Q1/70 ,  B82Y15/00

    CPC classification number: G01N21/658 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2400/043 ,  B01L2400/086 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/255 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/76 ,  G01N33/54333 ,  G01N33/587

    Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

    Abstract translation: 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

    Solid-phase chelators and electronic biosensors
    9.
    发明授权
    Solid-phase chelators and electronic biosensors 有权
    固相螯合剂和电子生物传感器

    公开(公告)号：US08962279B2

    公开(公告)日：2015-02-24

    申请号：US12655459

    申请日：2009-12-30

    Applicant: David J. Liu ,  Xing Su ,  Kai Wu

    Inventor： David J. Liu ,  Xing Su ,  Kai Wu

    IPC: C12P19/34 ,  C12M1/00 ,  C12Q1/48 ,  C12Q1/68 ,  G01N27/414

    CPC classification number: C12Q1/48 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N2333/9125 ,  C12Q2565/607 ,  C12Q2565/507 ,  C12Q2565/301

    Abstract: Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and electronically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced. Additionally, devices and methods are provided for detecting biomolecules through the detection of pyrophosphate ions.

    Abstract translation: 介绍了核酸测序方法。 通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。 在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸， 切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。 焦磷酸根离子被底物连接的螯合剂捕获并电子检测以确定待测序的DNA分子中下一个互补核酸的同一性。 另外，提供了通过检测焦磷酸根离子来检测生物分子的装置和方法。

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