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公开(公告)号：US20090075332A1
公开(公告)日：2009-03-19
申请号：US12047157
申请日：2008-03-12
申请人： Eugenio Ferrari , David A. Estell
发明人： Eugenio Ferrari , David A. Estell
CPC分类号： C12N9/54 , C12Y304/21062
摘要： This invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the present invention relates to methods for altering the expression of proteases in microorganisms, such as Bacillus species. The invention discloses modified polynucleotides, vectors, modified polypeptides, and processes for enhancing the production of proteases.
摘要翻译： 本发明涉及编码修饰的蛋白酶的修饰多核苷酸，以及改变微生物中蛋白酶生成的方法。 特别地，本发明涉及用于改变微生物如芽孢杆菌属物种中蛋白酶表达的方法。 本发明公开了修饰的多核苷酸，载体，修饰的多肽以及用于增强蛋白酶生产的方法。
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32.发明申请PROTEASES PRODUCING AN ALTERED IMMUNOGENIC RESPONSE AND METHODS OF MAKING AND USING THE SAME 审中-公开产生改变的免疫应答的蛋白质及其制备和使用方法公开(公告)号：US20090060933A1
公开(公告)日：2009-03-05
申请号：US11933027
申请日：2007-10-31
申请人： David A. Estell , Fiona A. Harding
发明人： David A. Estell , Fiona A. Harding
摘要： The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins.
摘要翻译： 与亲本蛋白质相比，本发明提供了表现出降低的免疫原性应答的新型蛋白质变体。 本发明还提供编码新变体的DNA分子，包含编码新变体的DNA的宿主细胞，以及用于制备蛋白质过敏原的方法。 此外，本发明提供了包含这些蛋白质的各种组合物，其比野生型蛋白质具有较少的免疫原性。
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公开(公告)号：US07396688B2
公开(公告)日：2008-07-08
申请号：US11011666
申请日：2004-12-14
IPC分类号： G01N24/00
CPC分类号： H01J49/0036 , Y10T436/24 , Y10T436/25125
摘要： The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separation technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.
摘要翻译： 本发明利用可用于确定粗溶液中生物聚合物浓度的特定生物聚合物的独特标签。 在优选的实施方案中，生物聚合物是蛋白质或多核苷酸。 特别地，本发明提供了通过与质谱联用的分离技术来测定和定量粗混合物中的生物分子的方法。 在一个一般实施方案中，选择靶生物分子用于分析，并且产生其类似物。 肽对的峰面积整合提供粗溶液中靶蛋白量的直接测量。
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公开(公告)号：US20080131932A1
公开(公告)日：2008-06-05
申请号：US11848213
申请日：2007-08-30
申请人： Hans De Nobel , David A. Estell , Wei Liu , Scott D. Power , Brian Schmidt , Huaming Wang
发明人： Hans De Nobel , David A. Estell , Wei Liu , Scott D. Power , Brian Schmidt , Huaming Wang
CPC分类号： C12N15/80 , C07K14/811
摘要： Described herein are protease inhibitors, variants thereof and methods for their production.
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公开(公告)号：US07329525B2
公开(公告)日：2008-02-12
申请号：US11014051
申请日：2004-12-16
申请人： David A. Estell
发明人： David A. Estell
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。
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公开(公告)号：US06794179B2
公开(公告)日：2004-09-21
申请号：US10188586
申请日：2002-07-02
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12N1511
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , D06M16/003
摘要： The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型金属蛋白酶的鉴定。 本发明提供了金属蛋白酶的核酸和氨基酸序列。 本发明还提供具有编码金属蛋白酶的基因的部分或全部的突变或缺失的宿主细胞。 本发明提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了用于处理包括本发明的金属蛋白酶的织物的清洁组合物，动物饲料和组合物。
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公开(公告)号：US06723550B1
公开(公告)日：2004-04-20
申请号：US09462845
申请日：2000-01-13
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12N948
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。
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公开(公告)号：US06300117B1
公开(公告)日：2001-10-09
申请号：US09308375
申请日：1999-05-14
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12N954
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , C12N15/75 , D06M16/003
摘要： The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.
摘要翻译： 本发明涉及革兰阳性微生物中新型金属蛋白酶（MP）的鉴定。 本发明提供芽孢杆菌（MP）的核酸和氨基酸序列。 本发明还提供具有编码MP的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的MP的清洁组合物。
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公开(公告)号：US5185258A
公开(公告)日：1993-02-09
申请号：US600430
申请日：1990-10-19
IPC分类号： C07K14/195 , C07K14/00 , C07K14/32 , C07K14/41 , C11D20060101 , C11D3/386 , C11D10/00 , C12N1/21 , C12N9/00 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/50 , C12N9/54 , C12N9/56 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/57 , C12N15/75 , C12P7/64 , C12P21/02
CPC分类号： C11D3/38609 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/50 , C12N9/54 , C12P7/6418 , C12R1/07
摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have properties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.
摘要翻译： 公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶突变体。 突变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中一个或多个氨基酸残基的取代。 这种突变型羰基水解酶具有与前体水解酶不同的性质，并且在洗涤剂配方中特别有用。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶中的位置+123和/或+ 274。
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40.发明授权Subtilisins modified at position 225 resulting in a shift in catalytic activity 失效在225位修饰的枯草杆菌蛋白酶导致催化活性的变化
公开(公告)号：US5155033A
公开(公告)日：1992-10-13
申请号：US294340
申请日：1989-01-06
IPC分类号： C12N9/56
CPC分类号： C12N9/54
摘要： There are described certain subtilisins wherein the amino acid sequence of such subtilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine, and serine, has been substituted for the amino acid residue naturally occurring at such position.
摘要翻译： 描述了某些枯草杆菌蛋白酶，其中这种枯草杆菌蛋白酶的氨基酸序列已经在解淀粉芽孢杆菌中相当于+225的位置被修饰，使得选自丙氨酸，亮氨酸，甲硫氨酸，谷氨酰胺，缬氨酸和丝氨酸的氨基酸 已取代天然存在于该位置的氨基酸残基。
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