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60 条记录 (耗时 0.045 秒)

    Cell-wall degrading enzyme variants
    31.
    发明授权
    Cell-wall degrading enzyme variants 失效
    细胞壁降解酶变体

    公开(公告)号：US06808915B2

    公开(公告)日：2004-10-26

    申请号：US10403192

    申请日：2003-03-31

    申请人： Sanne O Schroder Glad ,  Carsten Andersen ,  Martin Schulein ,  Torben Peter

    发明人： Sanne O Schroder Glad ,  Carsten Andersen ,  Martin Schulein ,  Torben Peter

    IPC分类号： C12N988

    CPC分类号： C11D3/38636 ,  C12N9/88

    摘要： The present invention relates to variants of a cell-wall degrading enzyme having a beta-helix structure, which variant has at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterizing the stack as interior or exterior; characterizing the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variant of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, D141 or E141, D145, K165, R194 and R199 when aligned with the pectate lyase comprising the amino acid sequence of SEQ ID NO: 2 are preferred.

    摘要翻译： 本发明涉及具有β-螺旋结构的细胞壁降解酶的变体，该变体具有至少一个取代基，其位置通过鉴定潜在属于堆叠的所有残基而确定; 将堆栈表征为内部或外部; 基于母体或野生型酶堆残基的主导特征和/或其相对于β-螺旋（内部或外部）的取向，将所述叠层表征为极性，疏水性或芳族/杂芳族化合物; 通过在属于这些基团之一的氨基酸的一个或所有位置内允许突变将堆叠的所有堆叠位置优化为疏水性脂族氨基酸，疏水性芳族或极性氨基酸; 通过DSC测量变体的热稳定性或应用相关测定，例如Pad-Steam应用测试; 并选择稳定的变体。 当与包含SEQ ID NO：1的氨基酸序列的果胶酸裂解酶比对时，具有保守氨基酸残基D111，D141或E141，D145，K165，R194和R199的野生型亲本果胶酸裂合酶（EC 4.2.2.2） 2是优选的。

    Endoglucanases
    33.
    发明授权
    Endoglucanases 失效
    内切葡聚糖酶

    公开(公告)号：US6001639A

    公开(公告)日：1999-12-14

    申请号：US651136

    申请日：1996-05-21

    申请人： Martin Schulein ,  Lene Nonboe Andersen ,  S.o slashed.ren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Iium Nielsen ,  Michiko Ihara ,  Shinobu Takagi

    发明人： Martin Schulein ,  Lene Nonboe Andersen ,  S.o slashed.ren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Iium Nielsen ,  Michiko Ihara ,  Shinobu Takagi

    IPC分类号： C12N15/09 ,  C05F11/08 ,  C05F17/00 ,  C07G99/00 ,  C11D3/386 ,  C12N1/19 ,  C12N9/42 ,  C12N15/00 ,  C12R1/645 ,  C12R1/69 ,  C12R1/77 ,  C12R1/80 ,  C12S3/04 ,  C12S11/00 ,  D06M13/02 ,  D06M13/322 ,  D06M13/46 ,  D06M16/00 ,  D06P5/02 ,  D06P5/15 ,  D21C5/00 ,  D21H11/16 ,  D21H11/20 ,  D21H17/00 ,  D21H21/10 ,  D21C1/00

    CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H21/10 ,  C07K2319/00 ,  D21H17/005 ,  Y02P20/145 ,  Y02W30/43

    摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequenceThr Arg Xaa Xaa Asp Cys Cys Xaa Xaa (SEQ ID NO:79) 1 2 3 4 5 6 7 8 9 - Xaa Cys Xaa Trp Xaa 10 11 12 13 14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequenceTrp Cys Cys Xaa Cys (SEQ ID NO:80) 1 2 3 4 5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译： 本发明涉及基本上由具有纤维素分解活性的酶组成的酶制剂，其包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列：Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa（SEQ ID NO：79） - 1 2 3 4 5 6 7 8 9 - - Xaa Cys Xaa Trp Xaa - 10 11 12 13 14 - 和由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列--Trp Cys Cys Xaa Cys（SEQ ID NO ：80） - 1 2 3 4 5 - 其中，在第一序列的位置3，氨基酸是Trp，Tyr或Phe; 在第一序列的位置4，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 分别位于第一序列的第9,10,12和14位，在第二序列的位置4中，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列 ，（i）当位置12的氨基残基为Ser时，14位的氨基酸残基不为Ser，（ii）当位置12的氨基残基为Gly时，位置14的氨基酸残基为 Ala在工业应用中表现非常好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。

    Cell-wall degrading enzyme variants
    37.
    发明申请
    Cell-wall degrading enzyme variants 审中-公开
    细胞壁降解酶变体

    公开(公告)号：US20050250181A1

    公开(公告)日：2005-11-10

    申请号：US10926709

    申请日：2004-08-26

    申请人： Sanne Schroder Glad ,  Carsten Andersen ,  Martin Schulein ,  Hanne Dela ,  Torben Frandsen

    发明人： Sanne Schroder Glad ,  Carsten Andersen ,  Martin Schulein ,  Hanne Dela ,  Torben Frandsen

    IPC分类号： C07H21/04 ,  C11D3/386 ,  C11D7/42 ,  C12N1/21 ,  C12N9/18 ,  C12N9/88 ,  C12N15/74

    CPC分类号： C11D3/38636 ,  C12N9/88

    摘要： The present invention relates to variants of a cell-wall degrading enzyme having a beta-helix structure, which variant has at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterizing the stack as interior or exterior; characterizing the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variants of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, D141 or E141, D145, K165, R194 and R199 when aligned with the pectate lyase comprising the amino acid sequence of SEQ ID NO: 2 are preferred.

    摘要翻译： 本发明涉及具有β-螺旋结构的细胞壁降解酶的变体，该变体具有至少一个取代基，其位置通过鉴定潜在属于堆叠的所有残基而确定; 将堆栈表征为内部或外部; 基于母体或野生型酶堆残基的主导特征和/或其相对于β-螺旋（内部或外部）的取向，将所述叠层表征为极性，疏水性或芳族/杂芳族化合物; 通过在属于这些基团之一的氨基酸的一个或所有位置内允许突变将堆叠的所有堆叠位置优化为疏水性脂族氨基酸，疏水性芳族或极性氨基酸; 通过DSC测量变体的热稳定性或应用相关测定，例如Pad-Steam应用测试; 并选择稳定的变体。 当与包含SEQ ID NO：1的氨基酸序列的果胶酸裂合酶比对时，具有保守氨基酸残基D111，D141或E141，D145，K165，R194和R199的野生型亲本果胶酸裂解酶（EC 4.2.2.2） 2是优选的。

    Family 44 xyloglucanases
    38.
    发明授权
    Family 44 xyloglucanases 有权
    家族44木葡聚糖酶

    公开(公告)号：US06815192B2

    公开(公告)日：2004-11-09

    申请号：US09784554

    申请日：2001-02-16

    申请人： Kirk Schnorr ,  Per Lina Jørgensen ,  Martin Schulein

    发明人： Kirk Schnorr ,  Per Lina Jørgensen ,  Martin Schulein

    IPC分类号： C12P2106

    CPC分类号： C12N9/24 ,  C11D3/38636 ,  C11D3/38645 ,  C12N9/2405 ,  C12N9/248 ,  C12Y302/01151 ,  D06L4/40 ,  D06M16/003

    摘要： The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.

    摘要翻译： 本发明涉及属于糖基水解酶家族44的木葡聚糖酶，并且具有pH5至pH8之间至少30％的相对木葡聚糖酶活性，来源于Paenibacillus属，特别是来自多粘杆菌（Paenibacillus polymyxa）或Paenibacillus sp。 木葡聚糖酶在常规洗涤剂组合物中表现出高性能。

    Pectate lyase
    39.
    发明授权
    Pectate lyase 失效
    果胶裂解酶

    公开(公告)号：US06124127A

    公开(公告)日：2000-09-26

    申请号：US73684

    申请日：1998-05-06

    申请人： Lene Nonboe Andersen ,  Martin Schulein ,  Niels Erik Krebs Lange

    发明人： Lene Nonboe Andersen ,  Martin Schulein ,  Niels Erik Krebs Lange

    IPC分类号： C12N9/40 ,  C12N9/88

    CPC分类号： C12N9/2465 ,  C12N9/88

    摘要： The present invention relates to microbial pectate lyases, more specifically to microbial enzymes exhibiting pectate lyase activity as their major enzymatic activity in the neutral and alkaline pH ranges, to a method of producing such enzymes, and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries.

    摘要翻译： 本发明涉及微生物果胶酸裂解酶，更具体地说，涉及在中性和碱性pH范围内显示出果胶裂解酶活性作为其主要酶活性的微生物酶，生产这种酶的方法，以及在纺织品中使用这些酶的方法， 洗涤剂和纤维素纤维加工业。