公开(公告)号：US06645717B1

公开(公告)日：2003-11-11

申请号：US09507548

申请日：2000-02-18

Applicant: Martin A. Smith ,  Mridula Iyer ,  Daqing Qu ,  James C. Davis

Inventor： Martin A. Smith ,  Mridula Iyer ,  Daqing Qu ,  James C. Davis

IPC: C12Q168

CPC classification number: C12N1/06 ,  B01L3/5023 ,  B01L3/5029 ,  B01L2300/0822 ,  B01L2300/163 ,  C12N15/1006 ,  C12Q1/6806 ,  G01N1/405 ,  Y10T436/143333 ,  C12Q2565/625

Abstract: A medium for storage and subsequent analysis of a genetic material includes a support for immobilizing the genetic material thereon and allowing subsequent elution of the genetic material therefrom and a coating functionally associated with the support for enabling cellular lysis and releasing the genetic material from the lysed cells while stabilizing the immobilized released genetic material. A method of storing the genetic material and subsequently analyzing the genetic material includes the steps of immobilizing the genetic material on a support while enabling cellular lysis and release of genetic material from the lysed cells and stabilizing the immobilized released genetic material on the support. The genetic material is then eluted to generate a soluble genetic material fraction. The eluted genetic material can be analyzed.

Abstract translation: 用于遗传物质的储存和随后分析的培养基包括用于将遗传物质固定在其上的载体，并允许随后从其中洗脱遗传物质和与支持物功能相关的涂层，以使细胞裂解并从裂解的细胞释放遗传物质 同时稳定固定化的释放的遗传物质。存储遗传物质并随后分析遗传物质的方法包括将遗传物质固定在载体上的步骤，同时能够从裂解的细胞中细胞裂解和释放遗传物质并稳定固定的释放 遗传物质的支持。 然后洗脱遗传物质以产生可溶性遗传物质级分。 可以分析洗脱的遗传物质。

公开(公告)号：US5164081A

公开(公告)日：1992-11-17

申请号：US588007

申请日：1990-09-25

Applicant: Randall W. Nichols ,  James C. Davis ,  Robert D. Littler

Inventor： Randall W. Nichols ,  James C. Davis ,  Robert D. Littler

IPC: B01D63/02 ,  B01D63/04 ,  B29C41/20 ,  B29C41/22 ,  B29C70/84

CPC classification number: B01D63/026 ,  B01D63/02 ,  B01D63/022 ,  B01D63/043 ,  B29C41/20 ,  B29C41/22 ,  B29C70/845

Abstract: A multi port apparatus (220,440) provides for the treatment of material. Such apparatus comprises at least one wafer (240,320,410), each wafer comprising a central area (246,325,416) providing a plurality of members (18,303,309,360) arranged in a plurality of layers (262,306,308,432) first peripheral support means (241,321,411) encompassing the central area and having upper and lower surfaces (244,245,324,414,415); second peripheral support means (248,326,418), having upper and lower surfaces (251,252,330,421,422) and carried radially outwardly from the first peripheral support means; and a plurality of separate bore fluid chambers (253-256, 335-338, 423-426) defined between the first and second peripheral support means and communicable with the central area; vessel means (228,448) providing a first port (221,441) a second port (222,442) and a plurality of separate bore fluid port means (223-226, 443-446) communicating with the four separate bore fluid chambers; and means for compressing (234,454) at least one wafer within the apparatus. The present invention also provides three and four port apparatus (60,180). Multi-chambered wafers (240,320,410) are provided as well as wafers (200,280,300,350 and 460) for use in the multi port apparatus. Finally, methods for the manufacture of wafers are provided.

Abstract translation: 多端口设备（220,440）提供材料的处理。 这种设备包括至少一个晶片（240,320,410），每个晶片包括中心区域（246,325,416），中心区域（246,325,416）提供布置在多个层（262,306,308,432）中的多个构件（18,303,309,360），第一周边支撑装置（241,321,411）包围中心区域并具有 上表面和下表面（244,245,324,414,415）; 第二周边支撑装置（248,326,418），具有上表面和下表面（251,252,330,421,422）并且从第一周边支撑装置径向向外承载; 和限定在第一和第二外围支撑装置之间且可与中央区域通信的多个单独的孔流体室（253-256,335-338,423-426） 提供第一端口（221,441）和第二端口（222,442）的容器装置（228,448）以及与四个单独的孔流体室连通的多个单独的孔流体端口装置（223-226,443-446） 以及用于在所述装置内压缩（234,454）至少一个晶片的装置。 本发明还提供三端口和四端口设备（60,180）。 提供多腔晶片（240,320,410）以及用于多端口设备的晶片（200,280,300,350和460）。 最后，提供了制造晶片的方法。

公开(公告)号：US4963264A

公开(公告)日：1990-10-16

申请号：US742872

申请日：1985-06-10

Applicant: James C. Davis

Inventor： James C. Davis

IPC: C22B3/24 ,  B01D15/00 ,  B01D15/02 ,  B01D15/08 ,  B01D61/24 ,  B01D61/26 ,  B01D61/30 ,  B01D61/32 ,  B01D61/58 ,  B01D65/10 ,  C07K1/32 ,  C08J5/22 ,  C10G31/11 ,  C10G33/06 ,  C13K1/04 ,  C13K11/00 ,  C22B3/00 ,  C22B15/00 ,  C22B26/10 ,  C22B26/20 ,  C22B59/00 ,  G01N30/02 ,  G01N30/84

CPC classification number: B01D15/00 ,  B01D15/361 ,  B01D61/243 ,  C07K1/32 ,  C10G31/11 ,  C10G33/06 ,  C13K1/04 ,  C13K11/00 ,  C22B3/24 ,  B01D15/3809 ,  B01D2311/04 ,  B01D2311/08 ,  Y02P10/234

Abstract: A process for the selective dialysis of liquid streams containing different materials comprises the steps of providing a porous membrane in a cell, the membrane having pores of sufficient dimension to allow passage of the different materials therethrough, circulating an affinity adsorbent stream against a first side of the membrane, where the adsorbent has a dimension larger than that of the pores and has a binding capacity for at least one but not all of the different materials and, passing the liquid stream containing the different materials against a second side of the membrane whereby certain of the materials become bound to the adsorbent upon passing through the membrane to the first side thereof and become separated from others of the materials which can return to the second side of the membrane. A separate stripping process is also provided which can be employed in conjunction with the dialysis process or alone where it is desirable to remove bound materials from an affinity adsorbent material.

Abstract translation: 用于选择性透析含有不同材料的液体流的方法包括在细胞中提供多孔膜的步骤，所述膜具有足够尺寸的孔，以允许不同材料通过，使亲和吸附剂流循环到第一侧 膜，其中吸附剂的尺寸大于孔的尺寸，并且具有至少一种但不是全部不同材料的结合能力，并且使含有不同材料的液体流抵靠膜的第二侧，由此某些 的材料在通过膜到其第一侧时与吸附剂结合，并且与能够返回到膜的第二侧的材料中的其它物质分离。 还提供单独的汽提过程，其可以与透析过程联合使用，或单独使用，其中期望从亲和吸附剂材料除去结合的材料。

公开(公告)号：US4770784A

公开(公告)日：1988-09-13

申请号：US802836

申请日：1985-11-29

Applicant: James C. Davis ,  Michael F. McGuiggan ,  Terry Berrett

Inventor： James C. Davis ,  Michael F. McGuiggan ,  Terry Berrett

IPC: B01D61/16 ,  B01D15/02 ,  B01D61/22 ,  B01D61/24 ,  B01D61/32 ,  B01D61/34 ,  B01D61/58 ,  B01D69/00 ,  B01D13/00

CPC classification number: B01D15/02 ,  B01D61/246 ,  B01D61/32 ,  B01D2215/021 ,  B01D2311/04 ,  B01D2311/08 ,  Y10S210/905 ,  Y10S210/908 ,  Y10S210/912

Abstract: A scrubbing process for high feed concentrations of different materials in liquid streams. The process calls for providing a porous membrane in a cell, the membrane having pores of sufficient dimension to allow passage of the different materials therethrough; providing a first stream comprising the different materials in a liquid carrier, providing a second stream comprising an affinity adsorbent in a second liquid carrier, wherein the affinity adsorbent has a dimension larger than that of the pores and has a preferred binding capacity for at least one but not all of the different materials; mixing the first and second streams together to form a composite mixture wherein a portion of the different materials become bound to the affinity adsorbent and a portion of the different materials remain essentially unbound to the affinity adsorbent; and passing the composite mixture against a first side of the membrane while passing a liquid carrier for any of the unbound material against a second side of the membrane to produce a first exit stream containing the affinity adsorbent and a high concentration of at least one of the different materials and a second exit stream containing the liquid carrier and a high concentration of at least one of the other different materials.

Abstract translation: 用于液体流中不同材料的高进料浓度的洗涤方法。 该方法要求在细胞中提供多孔膜，所述膜具有足够尺寸的孔以允许不同材料通过其中; 提供在液体载体中包含不同材料的第一流，在第二液体载体中提供包含亲和吸附剂的第二流，其中所述亲和吸附剂的尺寸大于孔的尺寸，并且对于至少一个 但不是全部不同的材料; 将第一和第二流混合在一起以形成复合混合物，其中不同材料的一部分与亲和吸附剂结合，并且不同材料的一部分基本上不结合到亲和吸附剂; 以及使所述复合混合物抵靠所述膜的第一侧，同时使任何未结合材料的液体载体抵靠所述膜的第二侧，以产生含有所述亲和吸附剂的第一出口流和高浓度的至少一种 不同的材料和含有液体载体的第二出口流和高浓度的至少一种其它不同的材料。

公开(公告)号：US4474664A

公开(公告)日：1984-10-02

申请号：US429357

申请日：1982-09-30

Applicant: Timothy S. Stevens ,  James C. Davis ,  Hamish Small

Inventor： Timothy S. Stevens ,  James C. Davis ,  Hamish Small

IPC: G01N30/60 ,  G01N30/84 ,  G01N30/96 ,  G01N31/04

CPC classification number: G01N30/84 ,  G01N30/96 ,  G01N2030/8435 ,  G01N2030/965

Abstract: A permselective ion-exchange membrane, preferably comprising hollow fiber(s) immersed within a regenerant, is used to selectively neutralize or suppress the conductivity of electrolyte in a stream of eluent also containing chromatographically separated ions. The invention particularly specifies a chromatographic separating column useful with an electrolyte eluent solution to separate ionic species by elution chromatography, wherein the effluent of the separator column is treated by the described membrane to suppress the conductivity of the electrolyte but not that of the separated ions.

Abstract translation: 优选包含浸入再生剂中的中空纤维的选择性选择性离子交换膜用于选择性中和或抑制还含有色谱分离的离子的洗脱液流中的电解质的导电性。 本发明特别规定了一种色谱分离柱，其用于电解液洗脱溶液以通过洗脱色谱分离离子物质，其中通过所述膜处理分离塔的流出物以抑制电解质的导电性，而不是分离离子的导电性。

公开(公告)号：US4451374A

公开(公告)日：1984-05-29

申请号：US465976

申请日：1983-02-14

Applicant: Dennis P. Peterson ,  James C. Davis

Inventor： Dennis P. Peterson ,  James C. Davis

IPC: G01N30/02 ,  B01D15/08 ,  B01J4/00 ,  B01J4/04 ,  B01J19/24 ,  G01N1/38 ,  G01N30/84

CPC classification number: G01N30/84 ,  B01D15/245 ,  B01J19/2475 ,  B01J4/001 ,  B01J4/04 ,  B01J2219/00159 ,  G01N2001/381 ,  G01N2030/8423 ,  G01N2030/8435

Abstract: Reagent is added to liquid chromatographic effluent to increase detection sensitivity of sample bands, or to enhance sensitivity with respect to interfering bands which overlap sample bands of interest, using one or more hollow fibers immersed within mobile reagent which is permeated through the walls of the fibers and, thus, ultimately diffused into the column effluent.

Abstract translation: 将试剂加入到液相色谱流出物中以提高样品带的检测灵敏度，或者使用浸渍在穿过纤维壁的移动试剂中的一种或多种中空纤维来增强与重叠样品带重叠的干扰条带的敏感性 并因此最终扩散到柱流出物中。