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公开(公告)号：US5888780A
公开(公告)日：1999-03-30
申请号：US802233
申请日：1997-02-19
IPC分类号： C07H21/00 , C07K14/47 , C07K14/82 , C12N9/12 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N15/10 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/686 , C12Q1/6886 , Y10S435/975
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known and unknown mutations, including single base changes, in nucleic acids. Methods are provided which allow for the identification of genetic mutations and the identification bacterial and viral strains and species in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 包括5'核酸酶和3'核酸外切酶在内的酶用于筛选核酸中已知和未知突变，包括单碱基变化。 提供了允许鉴定基因突变和鉴定样品中的细菌和病毒菌株和物种的方法。
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公开(公告)号：US5614402A
公开(公告)日：1997-03-25
申请号：US254359
申请日：1994-06-06
IPC分类号： C07H21/00 , C07K14/47 , C12N9/12 , C12N15/09 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , G01N33/53 , C12N9/22
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N9/1252 , C12N9/14 , C12Q1/682 , C12Q1/6823 , C12Q1/6825 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , C12Q1/705 , C12Q2600/156
摘要： A means cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
摘要翻译： 公开了以站点特异性方式切割核酸切割结构的方法。 采用具有5'核酸酶活性而不干扰核酸合成能力的切割酶作为检测特异性核酸序列的新方法的基础。
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公开(公告)号：US08026050B1
公开(公告)日：2011-09-27
申请号：US09660924
申请日：2000-09-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N9/1252 , C12N15/10 , C12N15/113 , C12Q1/682 , C12Q1/6823 , C12Q1/683 , C12Q1/689 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 酶包括5'核酸酶和3'核酸外切酶，用于检测和鉴定衍生自微生物的核酸。 提供了允许在样品中检测和鉴定细菌和病毒病原体的方法。
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公开(公告)号：US07273696B2
公开(公告)日：2007-09-25
申请号：US09941095
申请日：2001-08-28
IPC分类号： C12Q1/68
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N9/1252 , C12N15/10 , C12Q1/68 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , C12Q1/6853 , C12Q1/686 , C12Q1/689 , C12Q1/705 , Y10T436/143333 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 酶包括5'核酸酶和3'核酸外切酶，用于检测和鉴定衍生自微生物的核酸。 提供了允许在样品中检测和鉴定细菌和病毒病原体的方法。
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35.发明授权Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides 失效通过结构桥接寡核苷酸的核酸结构探测进行多态性分析公开(公告)号：US06709819B2
公开(公告)日：2004-03-23
申请号：US09825574
申请日：2001-04-03
申请人： Victor I. Lyamichev , Fang Dong , Mary Ann D. Brow , Lance Fors , Bruce P. Neri
发明人： Victor I. Lyamichev , Fang Dong , Mary Ann D. Brow , Lance Fors , Bruce P. Neri
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/133
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US5795763A
公开(公告)日：1998-08-18
申请号：US481238
申请日：1995-06-06
IPC分类号： C12N1/21 , C07H21/00 , C07K14/47 , C12N9/12 , C12N9/14 , C12N9/16 , C12N15/09 , C12N15/10 , C12N15/113 , C12N15/55 , C12Q1/44 , C12Q1/68 , C12Q1/70 , C12R1/19 , G01N33/53 , C12N9/22
CPC分类号： C12N9/14 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N15/113 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , C12Q1/705 , C12Q2600/156 , Y10T436/143333
摘要： A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
摘要翻译： 公开了以位点特异性方式切割核酸切割结构的方法。 采用具有5'核酸酶活性而不干扰核酸合成能力的切割酶作为检测特异性核酸序列的新方法的基础。
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公开(公告)号：US5719028A
公开(公告)日：1998-02-17
申请号：US789079
申请日：1997-02-06
IPC分类号： C07H21/00 , C07K14/47 , C07K14/82 , C12N9/12 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , C12Q1/44
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N15/10 , C12N9/1252 , C12Q1/6823 , C12Q1/6827
摘要： A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
摘要翻译： 公开了以位点特异性方式切割核酸切割结构的方法。 采用具有5'核酸酶活性而不干扰核酸合成能力的切割酶作为检测特异性核酸序列的新方法的基础。
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38.发明授权
公开(公告)号：US5541311A
公开(公告)日：1996-07-30
申请号：US73384
申请日：1993-06-04
IPC分类号： C12N1/21 , C07H21/00 , C07K14/47 , C12N9/12 , C12N9/14 , C12N9/16 , C12N15/09 , C12N15/10 , C12N15/113 , C12N15/55 , C12Q1/44 , C12Q1/68 , C12Q1/70 , C12R1/19 , G01N33/53 , C07H21/04 , C12N15/00
CPC分类号： C12N9/14 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N15/113 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , C12Q1/705 , C12Q2600/156 , Y10T436/143333
摘要： A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. Cleaving enzymes are produced through the use of novel DNA sequences which encode novel thermostable polymerases.
摘要翻译： 公开了以位点特异性方式切割核酸切割结构的方法。 采用具有5'核酸酶活性而不干扰核酸合成能力的切割酶作为检测特异性核酸序列的新方法的基础。 通过使用编码新型热稳定聚合酶的新型DNA序列产生切割酶。
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39.发明授权T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection 有权T结构侵入性切割测定，一致的核酸分配和低水平靶核酸检测公开(公告)号：US08354232B2
公开(公告)日：2013-01-15
申请号：US12840015
申请日：2010-07-20
申请人： Hatim Taysir Allawi , Vecheslav A. Elagin , Victor I. Lyamichev , Kwok Wu , Walter Iszczyszyn , Chris Fleming , LuAnne Chehak , Scott M. Law
发明人： Hatim Taysir Allawi , Vecheslav A. Elagin , Victor I. Lyamichev , Kwok Wu , Walter Iszczyszyn , Chris Fleming , LuAnne Chehak , Scott M. Law
CPC分类号： C12Q1/6823 , C12Q1/70 , C12Q2521/301 , C12Q2525/301
摘要： The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
摘要翻译： 本发明涉及用于低水平检测核酸的系统，方法和试剂盒，在单个反应容器中检测至少两种不同的病毒序列，并增加样品中病毒靶核酸的动态检测范围。 本发明还涉及T结构侵入性切割测定，以及T结构相关靶依赖性非靶扩增方法和组合物。 本发明还涉及用于将一致的核酸分配到表面上的方法，组合物，装置和系统。
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公开(公告)号：US20080199936A1
公开(公告)日：2008-08-21
申请号：US11926006
申请日：2007-10-28
IPC分类号： C12N9/00
CPC分类号： C12N9/1252 , A61K38/00 , C07K2319/02 , C12N9/22 , C12Q1/6823 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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