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143 条记录 (耗时 0.035 秒)

    Method for producing viral vectors
    32.
    发明申请
    Method for producing viral vectors 审中-公开
    生产病毒载体的方法

    公开(公告)号：US20070141705A1

    公开(公告)日：2007-06-21

    申请号：US10587123

    申请日：2005-01-20

    申请人： Makoto Inoue ,  Hiroshi Ban ,  Akihiro Iida ,  Mamoru Hasegawa

    发明人： Makoto Inoue ,  Hiroshi Ban ,  Akihiro Iida ,  Mamoru Hasegawa

    IPC分类号： C12N5/08 ,  C12N7/01 ,  C12N15/86

    CPC分类号： C12N7/00 ,  C12N2760/18851

    摘要： The present invention provides methods for producing viruses, whose propagation depends on the cleavage of viral protein by a protease, in a manner independent of the protease. The methods of the present invention for producing viruses comprise producing viruses in the presence of a modified viral protein in which the protease cleavage sequence is changed to a cleavage sequence for an alternative protease. Viral vectors can be more efficiently produced by replacing the protease cleavage sequence with a cleavage sequence for a protease expressed endogenously in virus-producing cells. The methods of the present invention enable the production of high titer viruses using a wide variety of cells.

    摘要翻译： 本发明提供了以与蛋白酶无关的方式生产病毒的方法，其传播依赖于蛋白酶对病毒蛋白的切割。 用于产生病毒的本发明的方法包括在修饰的病毒蛋白质的存在下产生病毒，其中将蛋白酶切割序列改变为用于替代性蛋白酶的切割序列。 通过用在病毒产生细胞内源性表达的蛋白酶的切割序列替换蛋白酶切割序列可以更有效地产生病毒载体。 本发明的方法能够使用多种细胞产生高滴度病毒。

    Method for transplanting lymphohematopoietic cells into mammal
    33.
    发明申请
    Method for transplanting lymphohematopoietic cells into mammal 审中-公开
    将淋巴造血细胞移植到哺乳动物中的方法

    公开(公告)号：US20060257381A1

    公开(公告)日：2006-11-16

    申请号：US10562322

    申请日：2004-06-25

    申请人： Keiya Ozawa ,  Yutaka Hanazono ,  Kyoji Ueda ,  Yasuji Ueda ,  Mamoru Hasegawa

    发明人： Keiya Ozawa ,  Yutaka Hanazono ,  Kyoji Ueda ,  Yasuji Ueda ,  Mamoru Hasegawa

    IPC分类号： A61K48/00 ,  C12N5/08

    CPC分类号： A01K67/0271 ,  A61K48/0066 ,  A61K2035/124 ,  C07K14/505 ,  C07K14/715 ,  C07K14/7153 ,  C07K2319/00 ,  C12N5/0647 ,  C12N2799/027

    摘要： The present invention provides a method for transplanting lymphohematopoietic cells into a mammal, which comprises the step of injecting cells into a bone marrow cavity, and wherein the cells have an exogenous gene encoding a receptor that induces cell proliferation in response to ligand binding. By combining intra-bone marrow transplantation (iBMT) and selective amplifier gene (SAG), marrow conditioning before the injection of the cells can be omitted. The present invention further provides a bone marrow transplant and a kit for transplanting lymphohematopoietic cells into mammals. Furthermore, the invention provides an SAG particularly suitable for such transplantation.

    摘要翻译： 本发明提供了将淋巴细胞生成细胞移植到哺乳动物中的方法，其包括将细胞注入骨髓腔中的步骤，并且其中细胞具有编码受体的外源基因，所述受体诱导响应于配体结合的细胞增殖。 通过组合骨髓移植（iBMT）和选择性放大基因（SAG），可以省略注射细胞前的骨髓调节。 本发明进一步提供骨髓移植和用于将淋巴造血细胞移植到哺乳动物中的试剂盒。 此外，本发明提供了特别适合于这种移植的SAG。

    Methods of examining (-) strand rna virus vectors having lowered ability to form grains and method of constructing the same
    35.
    发明申请
    Methods of examining (-) strand rna virus vectors having lowered ability to form grains and method of constructing the same 审中-公开
    检查具有降低形成颗粒能力的（ - ）链rna病毒载体的方法及其构建方法

    公开(公告)号：US20050130123A1

    公开(公告)日：2005-06-16

    申请号：US10489384

    申请日：2002-09-18

    申请人： Makoto Inoue ,  Yumiko Tokusumi ,  Akihiro Iida ,  Mamoru Hasegawa

    发明人： Makoto Inoue ,  Yumiko Tokusumi ,  Akihiro Iida ,  Mamoru Hasegawa

    IPC分类号： A61K48/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/45 ,  C12N15/86 ,  C12Q1/70 ,  C12N15/867 ,  C12Q1/68

    CPC分类号： C12N7/00 ,  A61K48/00 ,  C12N15/86 ,  C12N2760/18843 ,  C12N2760/18861 ,  C12N2800/30

    摘要： The present invention provides methods for testing and producing (−) strand RNA virus vectors with reduced or eliminated particle formation ability or cytotoxicity. It was revealed that a deficiency in M protein localization in cells introduced with such a (−) strand RNA virus vector could result in the suppression of virus-like particle (VLP) formation in the cells. The present invention provides methods for testing and screening for a (−) strand RNA virus vector in which particle formation ability has been reduced or eliminated, and methods for producing a recombinant (−) strand RNA virus vector in which particle formation ability has been reduced or eliminated. Such a vector, in which VLP formation has been reduced or eliminated, is extremely useful as a vector for gene therapy, since it neither induces cytotoxicity nor immune response due to the secondary release of viruses from cells in which it has been introduced.

    摘要翻译： 本发明提供了测试和生产（ - ）链RNA病毒载体的方法，其具有降低或消除的颗粒形成能力或细胞毒性。 显示出用这种（ - ）链RNA病毒载体引入的细胞中M蛋白定位的缺陷可导致细胞中病毒样颗粒（VLP）形成的抑制。 本发明提供了用于测定和筛选其中减少或消除了颗粒形成能力的（ - ）链RNA病毒载体的方法，以及用于生产其中颗粒形成能力已经降低的重组（ - ）链RNA病毒载体的方法 或消除。 已经减少或消除VLP形成的这种载体作为用于基因治疗的载体是非常有用的，因为它既不引起细胞毒性也不诱导免疫应答，这是由于病毒从其引入细胞的二次释放引起的。