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    Immunoassay for measuring the concentration of antigen in a sample
    31.
    发明授权
    Immunoassay for measuring the concentration of antigen in a sample 失效
    用于测量样品中抗原浓度的免疫测定

    公开(公告)号：US4824778A

    公开(公告)日：1989-04-25

    申请号：US867554

    申请日：1986-05-28

    申请人： Keiichi Nagai ,  Daizo Tokinaga ,  Kazumichi Imai ,  Kenji Yasuda ,  Satoshi Takahashi ,  Teruaki Kobayashi

    发明人： Keiichi Nagai ,  Daizo Tokinaga ,  Kazumichi Imai ,  Kenji Yasuda ,  Satoshi Takahashi ,  Teruaki Kobayashi

    IPC分类号： G01N33/543 ,  G01N33/561 ,  G01N33/53

    CPC分类号： G01N33/561 ,  Y10S435/968

    摘要： An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluorescent substance in the electrolyte solution.

    摘要翻译： 免疫测定法，其包括将抗体固定在用于电泳的基质中; 通过使抗原通过电泳移动的方法，将抗原固定在测量样品中，使其与上述固定化抗体进行抗原抗体反应; 通过电泳将标记抗体移动到上述固定化抗原上，使其与固定化抗原反应，或通过电泳将标记的抗原移动到上述固定化抗体的未反应部分，使其与未反应部分反应; 测定样品中的抗原浓度，其特征在于，将标记抗体或标记抗原的标记作为能够将底物覆盖成荧光物质的酶，通过电泳将所述酶转化为荧光物质， 使底物与标记酶反应，将其转化为荧光物质，并测量电解质溶液中荧光物质的浓度。

    ANALYZER
    33.
    发明申请
    ANALYZER 有权
    分析仪

    公开(公告)号：US20130316336A1

    公开(公告)日：2013-11-28

    申请号：US13983340

    申请日：2012-01-13

    申请人： Takuya Matsui ,  Ryoji Inaba ,  Kazumichi Imai ,  Ryusuke Kimura

    发明人： Takuya Matsui ,  Ryoji Inaba ,  Kazumichi Imai ,  Ryusuke Kimura

    IPC分类号： C12Q1/68

    CPC分类号： B01L3/502 ,  B01L3/0224 ,  B01L2300/026 ,  B01L2400/0406 ,  C12Q1/6869 ,  G01N35/0098 ,  G01N35/1002 ,  G01N35/1004 ,  G01N35/1095 ,  G01N2035/1039

    摘要： Provided is an analyzer capable of reducing the amount of wasted reagents and shortening time required for solution sending, thus increasing throughput for analysis. A microsyringe sucks a minimum required amount of reagent that is substantially the same amount of capacity of a flow cell to a sampling nozzle. Then, the sampling nozzle is inserted into an injection port of the flow cell, and the reagent is injected into the flow cell by driving the microsyringe. The inside of the sampling nozzle is cleaned by moving the sampling nozzle to the cleaning tank and ejecting cleaning water from the sampling nozzle, and the outside of the sampling nozzle is cleaned by spraying cleaning water from an inner wall of the cleaning tank.

    摘要翻译： 提供了能够减少试剂浪费量和溶液发送所需时间缩短的分析装置，从而提高分析用量。 微量注射器将最小所需量的试剂吸收到与采样喷嘴相当的流动池的容量基本上相同的量。 然后，将取样喷嘴插入流通池的注入口，通过驱动微量注射器将试剂注入到流通池中。 通过将采样喷嘴移动到清洗槽并从采样喷嘴喷射清洗水来清洁采样喷嘴的内部，并且通过从清洗槽的内壁喷射清洁水来清洁采样喷嘴的外部。

    Capillary electrophoresis apparatus
    35.
    发明授权
    Capillary electrophoresis apparatus 有权
    毛细管电泳仪

    公开(公告)号：US07883613B2

    公开(公告)日：2011-02-08

    申请号：US11806889

    申请日：2007-06-05

    申请人： Takashi Gomi ,  Ryoji Inaba ,  Motohiro Yamazaki ,  Hidenori Namba ,  Jin Matsumura ,  Hiromi Yamashita ,  Seiichi Ugai ,  Kazumichi Imai

    发明人： Takashi Gomi ,  Ryoji Inaba ,  Motohiro Yamazaki ,  Hidenori Namba ,  Jin Matsumura ,  Hiromi Yamashita ,  Seiichi Ugai ,  Kazumichi Imai

    IPC分类号： G01N27/453

    CPC分类号： G01N27/44721

    摘要： The present invention provides a capillary electrophoresis apparatus in which a capillary is easily attached to and detached from a migration medium filling unit without mixing impurities into the capillary. Mixture of impurities such as dust is also prevented when a capillary negative-electrode end is brought into contact with a sample stored in a vessel. Furthermore, temperature control is efficiently performed in the capillary. In the capillary electrophoresis apparatus, the whole of capillary array can be supported by grasping a grip portion by hand. A migration medium filling mechanism includes a slide mechanism which moves a polymer block with respect to a capillary head. The capillary electrophoresis apparatus includes a vessel in which a sample and a buffer can simultaneously be stored. Temperatures of the capillary and an irradiation and detection unit are controlled by a temperature control function provided in a thermostatic device.

    摘要翻译： 本发明提供了一种毛细管电泳装置，其中毛细管容易地连接到迁移介质填充单元并从迁移介质填充单元中分离而不将杂质混入毛细管中。 当毛细管负极端与存储在容器中的样品接触时，也可以防止诸如灰尘等杂质的混合物。 此外，在毛细管中有效地进行温度控制。 在毛细管电泳装置中，通过用手抓握把手部分可以支撑整个毛细管阵列。 迁移介质填充机构包括相对于毛细管头移动聚合物嵌段的滑动机构。 毛细管电泳装置包括可以同时存储样品和缓冲液的容器。 毛细管和照射和检测单元的温度由恒温装置中提供的温度控制功能控制。

    Capillary electrophoretic instrument and capillary array assembly
    37.
    发明授权
    Capillary electrophoretic instrument and capillary array assembly 有权
    毛细管电泳仪和毛细管阵列组件

    公开(公告)号：US06572752B1

    公开(公告)日：2003-06-03

    申请号：US09671818

    申请日：2000-09-27

    申请人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

    发明人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

    IPC分类号： G01N27453

    CPC分类号： G01N27/44713 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782

    摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.

    摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。通过将设置在每个毛细管附近的电极安装在位于 样品板（在孔的区域内）与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

    Capillary array electrophoresis system
    39.
    发明授权
    Capillary array electrophoresis system 失效
    毛细管阵列电泳系统

    公开(公告)号：US5938908A

    公开(公告)日：1999-08-17

    申请号：US847735

    申请日：1997-04-22

    申请人： Takashi Anazawa ,  Hideki Kambara ,  Satoshi Takahashi ,  Kazumichi Imai ,  Hisanori Nasu

    发明人： Takashi Anazawa ,  Hideki Kambara ,  Satoshi Takahashi ,  Kazumichi Imai ,  Hisanori Nasu

    IPC分类号： G01N21/64 ,  B01D57/02 ,  G01N27/447 ,  G01N35/00 ,  G01N27/26

    CPC分类号： G01N27/44721 ,  G01N27/44782 ,  G01N2035/00237

    摘要： A capillary electrophoresis system comprising a plurality of capillaries filled with a migration medium in which samples to which fluorephore labels are added migrate, a light source providing an excitation light exciting the fluorephore labels, a photo detector detecting a fluorescence radiated from the fluorephore labels, and light focusing means placed between the plurality of capillaries to which the excitation light is irradiated. The parts of the plurality of capillaries to which the excitation light is irradiated and the light focusing means are arranged in a plane shape. The excitation light is irradiated through the plurality of capillaries and the light focusing means, the capillaries and light focusing means alternatively placed in the parts where an excitation light is irradiated. The light focusing means consisting of cylindrical rod lenses. The axis of the cylindrical rod lenses is placed substantially parallel with the capillaries, which can detect the fluorescence from samples by irradiating a plurality of capillaries substantially at the same time in a batch without mechanical scanning of a plurality of capillaries or without optical scanning of a light beam, providing a highly sensitive detection of samples.

    摘要翻译： 一种毛细管电泳系统，包括填充有迁移介质的多个毛细管，其中添加有荧光标记的样品迁移，提供激发荧光标记的激发光的光源，检测从荧光标记物辐射的荧光的光电检测器，以及 放置在激发光照射到的多个毛细管之间的光聚焦装置。 照射激发光的多个毛细管的部分和光聚焦装置被布置成平面形状。 激发光通过多个毛细管和光聚焦装置照射，毛细管和光聚焦装置交替地放置在照射激发光的部分中。 光聚焦装置由圆柱形棒状透镜组成。 圆柱形棒状透镜的轴线基本上与毛细管平行放置，其可以通过基本上同时照射多个毛细管而检测来自样品的荧光，而不需要对多个毛细管进行机械扫描，或者不进行光学扫描 光束，提供高灵敏度的样品检测。

    Immunoassay method
    40.
    发明授权
    Immunoassay method 失效
    免疫测定法

    公开(公告)号：US4628035A

    公开(公告)日：1986-12-09

    申请号：US638423

    申请日：1984-08-07

    申请人： Daizo Tokinaga ,  Teruaki Kobayashi ,  Kazumichi Imai

    发明人： Daizo Tokinaga ,  Teruaki Kobayashi ,  Kazumichi Imai

    IPC分类号： A61K39/44 ,  G01N33/561 ,  G01N33/58 ,  G01N33/543

    CPC分类号： G01N33/561

    摘要： An immunoassay method for measuring a concentration of an antigen for a short period of time by immobilizing an antibody over the whole zone of an effective supporting matrix for electrophoresis and fixing an antigen in a sample to be measured by electrophoresis for the antigen-antibody reaction between said immobilized antibody and said antigen.

    摘要翻译： 一种免疫测定方法，用于通过将抗体固定在有效支持基质的整个区域上进行电泳和固定待测样品中的抗原，将抗体固定在短时间内的抗原浓度，以通过电泳进行抗原抗体反应 所述固定化抗体和所述抗原。