公开(公告)号：US20170038398A1

公开(公告)日：2017-02-09

申请号：US15099388

申请日：2016-04-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Vladimir MARTIN

IPC: G01N33/84 ,  G01N33/58

CPC classification number: G01N33/84 ,  A61K31/353 ,  C07D311/90 ,  C07D413/04 ,  C07D413/10 ,  C07D491/04 ,  C07D491/147 ,  C09B11/22 ,  C09B11/24 ,  G01N33/582 ,  G01N33/6872 ,  G01N2223/406 ,  G01N2458/00

Abstract: Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (Mc), a reporter molecule and two or more lipophilic groups (GL) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.

Abstract translation: 描述了细胞可渗透金属离子指示剂化合物及其使用和合成方法。 化合物包括金属螯合部分（Mc），报告分子和通过接头共价键合到报告分子上的两个或多个亲油基团（GL），其中亲和基团当存在于活细胞中时被切割，导致两个 或更多带负电的组。

公开(公告)号：US20170037248A1

公开(公告)日：2017-02-09

申请号：US15299841

申请日：2016-10-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert Batchelor ,  Yue GE ,  Iain JOHNSON ,  Wai-Yee LEUNG ,  Jixiang LIU ,  Brian PATCH ,  Peter SMALLEY ,  Thomas STEINBERG ,  Kyle GEE

IPC: C09B19/00 ,  C12Q1/28 ,  C07D495/04 ,  C07D265/38 ,  C07D413/12

CPC classification number: C09B19/00 ,  C07D265/38 ,  C07D413/12 ,  C07D495/04 ,  C12Q1/28

Abstract: The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.

Abstract translation: 本发明提供可用于各种测定形式的新型氟化聚砜化合物。 还提供了使用包含本发明化合物的化合物和试剂盒的方法以及详细说明化合物在一种或多种测定形式中的用途的说明书。

公开(公告)号：US20150037834A1

公开(公告)日：2015-02-05

申请号：US14455550

申请日：2014-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel BEACHAM ,  Jeffrey DZUBAY ,  Kyle GEE ,  Vladimir MARTIN ,  Aleksey RUKAVISHNIKOV

IPC: G01N33/50

CPC classification number: G01N33/84 ,  C07D209/14 ,  C07D311/82 ,  C07D311/90 ,  C07D405/12 ,  C07D413/04 ,  C07D491/22 ,  C07F5/02 ,  C07K1/13 ,  C09B7/00 ,  C09B11/12 ,  C09B11/24 ,  C09B11/28 ,  C09B57/00 ,  C09B69/00 ,  C09K11/06 ,  G01N31/22 ,  G01N33/5005 ,  G01N33/5058 ,  G01N33/5091 ,  G01N33/582 ,  G01N33/80 ,  G01N2333/245 ,  Y10T436/143333

Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.

Abstract translation: 描述了一类新的pH敏感荧光染料和与其相关的测定。 染料和测定特别适用于生物学应用，包括吞噬和监测细胞内过程。 本发明的pH敏感荧光染料包括式I化合物：其中在整个申请中描述了变量。

公开(公告)号：US20230120665A1

公开(公告)日：2023-04-20

申请号：US17816043

申请日：2022-07-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel BEACHAM ,  Kyle GEE

IPC: G01N33/569 ,  C12N15/09

Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.

公开(公告)号：US20220119701A1

公开(公告)日：2022-04-21

申请号：US17514492

申请日：2021-10-29

Applicant: Life Technologies Corporation

Inventor： Adam YORK ,  Eric WELCH ,  Kyle GEE ,  Xin WANG ,  Robert AGGELER ,  Yi-Zhen HU

IPC: C09K11/02 ,  C09B67/00 ,  C09K11/06 ,  G01N33/50 ,  G01N33/533 ,  G01N33/58 ,  H04L12/18

Abstract: Water-soluble, fluorescent particles and compositions, kits, and methods of making and using such particles are disclosed. Processes for preparing fluorescent particles and for controlling the size, polydispersity and optical properties of such particles also are provided.

公开(公告)号：US20200179541A1

公开(公告)日：2020-06-11

申请号：US16713388

申请日：2019-12-13

Applicant: Life Technologies Corporation

Inventor： Brian AGNEW ,  Robert AGGELER ,  Hee Chol KANG ,  Aimei CHEN ,  Kyle GEE

IPC: A61K51/10 ,  G01N33/534 ,  G01N33/68 ,  C07K14/435 ,  C07K16/18 ,  C12P21/00 ,  C12P21/06 ,  G01N33/58

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

公开(公告)号：US20190339280A1

公开(公告)日：2019-11-07

申请号：US16516545

申请日：2019-07-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Levente EGRY ,  Kyle GEE

IPC: G01N33/68 ,  C07F5/02 ,  G01N33/58 ,  C09B57/00 ,  C09B23/04 ,  G01N33/52

Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.

公开(公告)号：US20190154696A1

公开(公告)日：2019-05-23

申请号：US15753454

申请日：2016-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott CLARKE ,  Kyle GEE ,  Carmen FINNESSY

IPC: G01N33/58 ,  C12Q1/6813 ,  G01N33/52

Abstract: The present disclosure provides methods, compositions and kits for measuring cellular nascent nucleic acid synthesis by colorimetric labeling of nucleic acid. The nucleic acid synthesis can be measured as cell proliferation, DNA or RNA synthesis, gene expression or apoptosis. Additionally, these methods may be used to screen compounds for their effect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.

公开(公告)号：US20180222855A1

公开(公告)日：2018-08-09

申请号：US15894057

申请日：2018-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun ZHOU ,  Kyle GEE ,  Yolanda TENNICO ,  Peter SLADE ,  Hee Chol KANG ,  Shaheer KHAN ,  Brian EVANS ,  James STRAY

IPC: C07C309/47 ,  C07D311/84 ,  C07C309/51 ,  G01N33/58

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US20180196033A1

公开(公告)日：2018-07-12

申请号：US15738069

申请日：2016-06-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Penny MELQUIST ,  Kyle GEE

IPC: G01N33/50 ,  C09B57/02

CPC classification number: G01N33/5005 ,  C09B57/02 ,  G01N33/582

Abstract: Described herein are compounds, methods, and kits for short- and long-term tracking of cell proliferation, differentiation, and/or function. The compounds disclosed herein are coumarin-based cell-tracking reagents that fluoresce in the blue portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, good retention within cells and low toxicity toward cells.