公开(公告)号：US20170059516A1

公开(公告)日：2017-03-02

申请号：US15351253

申请日：2016-11-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd REARICK ,  Jeremy Jordan ,  John Nobile ,  William Mileski ,  Chun Heen Ho

IPC: G01N27/414 ,  B01L3/00

CPC classification number: G01N27/4145 ,  B01L3/502 ,  B01L3/52 ,  B01L7/00 ,  B01L2200/0605 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2300/1894 ,  B01L2400/0487 ,  B01L2400/0622 ,  G01N27/414 ,  G01N31/20 ,  G05D23/19 ,  G05D23/1931

Abstract: An apparatus for detecting chemical reactions may be provided. The apparatus may comprise a chemical detection device. The chemical detection device may include a chemical sensor, which may be mounted on the chemical detection device. The apparatus may further comprise a valve block. The valve block may fluidly couple a plurality of reagent containers to the chemical detection device. The apparatus may further comprise a heat exchanger and a controller. The controller may control a fluid connection between the valve block and the chemical detection device. The controller may be also configured to adjust a temperature of a selected reagent from the plurality of reagent containers via the heat exchanger. The temperature of the selected reagent may be adjusted prior to the reagent entering the chemical detection device.

Abstract translation: 可以提供用于检测化学反应的装置。 该装置可以包括化学检测装置。 化学检测装置可以包括可安装在化学检测装置上的化学传感器。 该装置还可以包括阀块。 阀块可以将多个试剂容器流体耦合到化学检测装置。 该装置还可以包括热交换器和控制器。 控制器可以控制阀块和化学检测装置之间的流体连接。 控制器还可以被配置为经由热交换器从多个试剂容器调节所选择的试剂的温度。 可以在试剂进入化学检测装置之前调整所选择的试剂的温度。

公开(公告)号：US20150299787A1

公开(公告)日：2015-10-22

申请号：US14754231

申请日：2015-06-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  David LIGHT ,  Todd REARICK ,  James A. BALL

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  G01N27/4145

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

Abstract translation: 本公开涉及新型颗粒组合物和制备所述组合物在核酸分析中的应用的方法，以及用于其的装置和系统。

公开(公告)号：US20150031558A1

公开(公告)日：2015-01-29

申请号：US14515403

申请日：2014-10-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd REARICK ,  Jessica Lynn REED ,  Jason GIOIA ,  Devin DRESSMAN ,  Nicholas HAPSHE ,  Brian REED ,  John Andrew SHERIDAN

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0893 ,  C12Q1/6837 ,  G01N33/50 ,  G01N33/543

Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.

Abstract translation: 提供了用于将样品沉积到表面上的方法，组合物，系统，装置和试剂盒。 样品可以包括一个或多个颗粒，并且该表面可以包括一个或多个反应室。 在一些实施方案中，沉积可以包括使用伴随颗粒与样品颗粒的组合。

公开(公告)号：US20140327474A1

公开(公告)日：2014-11-06

申请号：US14333754

申请日：2014-07-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeremy JORDAN ,  Todd REARICK

IPC: H03K17/16 ,  H03K19/003 ,  G01N27/414

CPC classification number: H03K17/162 ,  G01N27/4145 ,  G06F1/06 ,  G06F1/26 ,  H03K19/00346

Abstract: A system including a power supply and a clock circuitry to generate a plurality of clock signals. Each clock signal is synchronous with a primary clock signal. First, second, and third clock signals of the plurality of clock signals are asynchronous to each other. The system further includes a plurality of switches. Each switch of the plurality of switches is communicatively coupled to the power supply and the clock circuitry. A first switch of the plurality of switches receives the first clock signal, a second switch of the plurality of switches receives the second clock signal, and a third switch of the plurality of switches receives the third clock signal.

Abstract translation: 一种包括电源和时钟电路以产生多个时钟信号的系统。 每个时钟信号与主时钟信号同步。 多个时钟信号的第一，第二和第三时钟信号彼此异步。 该系统还包括多个开关。 多个开关中的每个开关通信地耦合到电源和时钟电路。 多个开关的第一开关接收第一时钟信号，多个开关中的第二开关接收第二时钟信号，并且多个开关中的第三开关接收第三时钟信号。

公开(公告)号：US20140202883A1

公开(公告)日：2014-07-24

申请号：US14162612

申请日：2014-01-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John NOBILE ,  George T. ROTH ,  Todd REARICK ,  Jonathan M. SCHULTZ ,  Jonathan ROTHBERG ,  David MARRAN

IPC: G01N27/30

CPC classification number: G01N27/301 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00722 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

Abstract: The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors under minimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles. Such detection of analyte- or particle-containing microwells may be used as a step in additional noise reduction methods.

Abstract translation: 本发明涉及用于在最小噪声条件下向多个电子传感器阵列上进行的多个分析反应的多个试剂的输送和监测的装置和方法。 一方面，本发明提供了通过减去从相邻传感器测得的输出信号的平均值来提高来自电子传感器感测分析物或反应副产物的输出信号的信噪比的方法，其中分析物或反应副产物不存在。 在其它方面，本发明提供了与微孔阵列集成的电子传感器阵列，用于限制用于分析反应的分析物和/或颗粒，以及通过使传感器活性试剂通过阵列而识别含有分析物和/或颗粒的微孔的方法，以及 将传感器响应时间与分析物或颗粒的存在或不存在相关联。 含有分析物或含微粒微孔的这种检测可以用作额外的降噪方法中的步骤。

公开(公告)号：US20130231870A1

公开(公告)日：2013-09-05

申请号：US13754566

申请日：2013-01-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Charles SUGNET ,  Simon CAWLEY ,  Mohit GUPTA ,  Iztok MARJANOVIC ,  Todd REARICK ,  Mark BEAUCHEMIN ,  Earl HUBBELL

IPC: H03M7/30

CPC classification number: H03M7/30 ,  G06F19/24

Abstract: A compression method includes measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises at least one region associated with expected measured values and at least one region associated with unpredictable measured values; applying a first compression process to the waveform, the first compression process including an averaging of one or more frames in one or more portions of the waveform; and applying a second compression process to the waveform, the second compression process including a truncating of data corresponding to a portion of the waveform that is not related to a nucleotide incorporation component of the waveform.

Abstract translation: 压缩方法包括测量与传感器阵列上发生的化学事件相关联的波形，其中所述波形包括与预期测量值相关联的至少一个区域和与不可预测的测量值相关联的至少一个区域; 对所述波形应用第一压缩处理，所述第一压缩处理包括所述波形的一个或多个部分中的一个或多个帧的平均值; 以及对所述波形应用第二压缩处理，所述第二压缩处理包括对应于与所述波形的核苷酸掺入成分无关的所述波形的一部分的数据的截断。