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215 条记录 (耗时 0.031 秒)

    Process for producing fructose 2,6-bisphosphate and purification process thereof
    31.
    发明授权
    Process for producing fructose 2,6-bisphosphate and purification process thereof 失效
    制备2,6-二磷酸果糖的方法及其纯化方法

    公开(公告)号：US5501965A

    公开(公告)日：1996-03-26

    申请号：US213738

    申请日：1994-03-16

    申请人： Ken Iwata ,  Tatsuo Katayama ,  Hiroshi Nakajima

    发明人： Ken Iwata ,  Tatsuo Katayama ,  Hiroshi Nakajima

    IPC分类号： C07H11/04 ,  C12N9/12 ,  C12P19/02 ,  C12P19/04 ,  C12P19/24

    CPC分类号： C12N9/1205 ,  C07H11/04 ,  C12P19/02 ,  C12P19/04

    摘要： This invention relates to a process for the production of fructose 2,6-bisphosphate which comprises effecting reactions among (i) fructose 6-phosphate, (ii) glucose, (iii) fructose or (iv) glucose 6-phosphate, ATP and a phosphate donor, in the presence of (i) fructose 6-phosphate 2-kinase (PFK 2) and an enzyme which converts ADP into ATP (ADP/ATP converting enzyme), (ii) PFK 2, an ADP/ATP converting enzyme, hexokinase or glucokinase and glucose 6-phosphate isomerase, (iii) PFK 2, an ADP/ATP converting enzyme and hexokinase or glucokinase, or (iv) PFK 2, an ADP/ATP converting enzyme and glucose 6-phosphate isomerase; to a process for the production of fructose 2,6-bisphosphate which comprises allowing diesterase to coexist in a solution containing fructose 1,2-cyclic, 6-bisphosphate; and to a process for the purification of fructose 2,6-bisphosphate which comprises adding zinc salt to a solution containing fructose 2,6-bisphosphate, removing formed precipitate of impurities and adding a zinc salt to the resulting solution to isolate formed precipitate of fructose 2,6-bisphosphate.According to the present invention, fructose 2,6-bisphosphate can be produced easily with a high yield and high purity fructose 2,6-bisphosphate can be obtained easily.

    摘要翻译： 本发明涉及一种生产2,6-二磷酸果糖的方法，其包括（i）果糖-6-磷酸，（ii）葡萄糖，（iii）果糖或（iv）葡萄糖-6-磷酸，ATP和 磷酸供体，在（i）果糖-6-磷酸2-激酶（PFK 2）和将ADP转化成ATP（ADP / ATP转化酶）的酶的存在下，（ii）PFK 2，ADP / ATP转化酶， （iii）PFK 2，ADP / ATP转化酶和己糖激酶或葡糖激酶，或（iv）PFK 2，ADP / ATP转化酶和葡萄糖-6-磷酸异构酶; 涉及生产2,6-二磷酸果糖的方法，其包括使二酯酶共存于含有1,2-环状6-二磷酸果糖的溶液中; 以及纯化2,6-二磷酸果糖的方法，该方法包括向含有2,6-二磷酸果糖的溶液中加入锌盐，除去形成的杂质沉淀物并向所得溶液中加入锌盐以分离形成的果糖沉淀物 2,6-二磷酸酯。 根据本发明，可以容易地以高产率制备2,6-二磷酸果糖，并且可以容易地获得高纯度的2,6-二磷酸果糖。

    Method for producing 3'-phosphoadenosine 5'-phosphosulfate
    34.
    发明授权
    Method for producing 3'-phosphoadenosine 5'-phosphosulfate 失效
    产生3'-磷酸腺苷5'-磷酸硫酸的方法

    公开(公告)号：US5376535A

    公开(公告)日：1994-12-27

    申请号：US974216

    申请日：1992-11-10

    申请人： Masaaki Onda ,  Hiroshi Ibuki ,  Hiroshi Nakajima

    发明人： Masaaki Onda ,  Hiroshi Ibuki ,  Hiroshi Nakajima

    IPC分类号： C12P17/18 ,  C12N9/12 ,  C12P19/32 ,  C12R1/01 ,  C12R1/07 ,  C12P19/30

    CPC分类号： C12N9/12 ,  C12P19/32

    摘要： A method for producing 3'-phosphoadenosine 5'-phosphosulfate, which comprises (1) reacting adenosine 5'-triphosphate with a sulfate donor in the presence of heat-stable adenosine 5'-triphosphate sulfurylase to produce adenosine 5'-phosphosulfate, and (2) reacting said adenosine 5'-phosphosulfate with adenosine 5'-triphosphate in the presence of heat-stable adenosine 5'-phosphosulfate kinase; and another method for producing 3'-phosphoadenosine 5'-phosphosulfate which comprises (1) reacting adenosine 5'-triphosphate with a sulfate donor in the presence of adenosine 5'-triphosphate sulfurylase to produce adenosine 5'-phosphosulfate, (2) reacting said adenosine 5'-phosphosulfate with adenosine 5'-triphosphate in the presence of adenosine 5'-phosphosulfate kinase to produce 3'-phosphoadenosine 5'-phosphosulfate and adenosine 5'-diphosphate, and (3) converting said adenosine 5'-diphosphate into adenosine 5'-triphosphate in the presence of a phosphate donor and an enzyme capable of converting adenosine 5'-diphosphate into adenosine 5'-triphosphate are disclosed.

    摘要翻译： 一种产生3'-磷酸腺苷5'-磷酸硫酸的方法，其包括（1）在热稳定的腺苷5'-三磷酸硫酸化酶存在下使腺苷5'-三磷酸与硫酸盐供体反应以产生腺苷5'-磷酸硫酸，以及 （2）在热稳定的腺苷5'-磷酸硫酸激酶的存在下使所述腺苷5'-磷酸硫酸与腺苷5'-三磷酸反应; 另外一种产生3'-磷酸腺苷5'-磷酸硫酸的方法，它包括（1）在5'-三磷酸磷酸腺苷酸腺苷存在下使腺苷5'-三磷酸与硫酸盐供体反应，产生腺苷5'-磷酸硫酸，（2） 在腺苷5'-磷酸硫酸激酶的存在下，腺苷5'-磷酸硫酸腺苷与腺苷5'-磷酸三磷酸腺苷产生3'-磷酸腺苷5'-磷酸硫酸和腺苷5'-二磷酸，和（3）转化所述腺苷5'-二磷酸 在磷酸盐供体和能够将腺苷5'-二磷酸腺苷转化为腺苷5'-三磷酸的酶存在下，转化为腺苷5'-三磷酸。

    New restriction enzyme and process for producing the same
    38.
    发明授权
    New restriction enzyme and process for producing the same 失效
    新的限制酶及其制备方法

    公开(公告)号：US4808531A

    公开(公告)日：1989-02-28

    申请号：US756380

    申请日：1985-07-18

    申请人： Akira Obayashi ,  Nobutsugu Hiraoka ,  Keiko Kita ,  Hiroshi Nakajima

    发明人： Akira Obayashi ,  Nobutsugu Hiraoka ,  Keiko Kita ,  Hiroshi Nakajima

    IPC分类号： C12N15/09 ,  C12N9/16 ,  C12N9/22 ,  C12R1/01 ,  C12N9/14 ,  C12N9/00 ,  C12P21/00

    CPC分类号： C12N9/22 ,  Y10S435/822

    摘要： The invention provides restriction endonuclease Mf1 I capable of recognizing the base sequence as shown below on a double-stranded DNA molecule and cleaving the DNA chain at the arrow-marked positions, but has no such action when A is methylated5'--Pu.dwnarw.GATC Py--3'3'--Py CTAG.uparw.Pu--5'(wherein A represents adenosine, G guanosine, T thymidine, C cytidine, Pu adenosine or guanosine, and Py thymidine or cytidine). The restriction endonuclease is produced by culturing Microbacterium flavum IAM 1642, FERM BP-938 in a culture medium and recovering it from the culture.

    摘要翻译： 本发明提供了限制性内切核酸酶Mf1I，其能够识别如下所示的碱基序列在双链DNA分子上并且在箭头标记位置处切割DNA链，但当A是甲基化5'-Pu＆darr＆GATC时没有这样的作用 Py-3'3'-Py CTAG＆uarr＆Pu-5'（其中A表示腺苷，G鸟苷，T胸苷，C胞苷，Pu腺苷或鸟苷，Py胸苷或胞苷）。 限制性内切酶通过在培养基中培养黄色微生物IAM1642，FERM BP-938并从培养物中回收来产生。

    Fuel cell catalyst layer and uses thereof
    40.
    发明授权
    Fuel cell catalyst layer and uses thereof 有权
    燃料电池催化剂层及其用途

    公开(公告)号：US09570757B2

    公开(公告)日：2017-02-14

    申请号：US14240106

    申请日：2011-09-09

    申请人： Yasuaki Wakizaka ,  Ryuji Monden ,  Takuya Imai ,  Hiroshi Nakajima

    发明人： Yasuaki Wakizaka ,  Ryuji Monden ,  Takuya Imai ,  Hiroshi Nakajima

    IPC分类号： H01M8/02 ,  H01M4/86 ,  H01M4/92 ,  H01M8/10

    CPC分类号： H01M4/8657 ,  H01M4/8605 ,  H01M4/925 ,  H01M2008/1095 ,  Y02E60/50

    摘要： Provided is a fuel cell catalyst layer which has a catalytic performance equivalent to or higher than fuel cell catalyst layers containing platinum alone and which is inexpensive. The fuel cell catalyst layer of the present invention includes a metal oxycarbonitride-containing layer (I) and a platinum-containing layer (II). It is preferable that the mass ratio per unit area of the metal oxycarbonitride in the layer (I) to platinum in the layer (II) (metal oxycarbonitride/platinum) is 2 to 500. It is preferable that the mass per unit area of platinum in the layer (II) is 0.005 to 0.2 mg/cm2.

    摘要翻译： 本发明提供一种燃料电池用催化剂层，该催化剂层的催化性能等于或高于单独含有铂的燃料电池催化剂层，其价格便宜。 本发明的燃料电池催化剂层包括含有含碳氮氧化物的层（I）和含铂层（II）。 层（I）中的层（I）中的铂的金属碳氮氧化物的单位面积质量比（金属碳氮氧化物/铂）优选为2〜500。优选铂单位面积的质量 在层（II）中为0.005〜0.2mg / cm 2。