公开(公告)号：US20170326174A1

公开(公告)日：2017-11-16

申请号：US15640104

申请日：2017-06-30

申请人： Michael C. Holmes ,  Fyodor Urnov

发明人： Michael C. Holmes ,  Fyodor Urnov

IPC分类号： A61K35/12 ,  C07K14/715 ,  C12N15/85 ,  C12N9/22 ,  C12N9/14 ,  C07K14/435 ,  C07K14/47 ,  C12N15/62

摘要： Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.

公开(公告)号：US09506120B2

公开(公告)日：2016-11-29

申请号：US12284887

申请日：2008-09-25

申请人： Yannick Doyon ,  Fyodor Urnov

发明人： Yannick Doyon ,  Fyodor Urnov

IPC分类号： C12Q1/34 ,  C12Q1/68 ,  C12N9/22 ,  G01N33/573

CPC分类号： C12Q1/6897 ,  C12N9/22 ,  C12Q1/34 ,  G01N33/573 ,  G01N2333/922

摘要： Disclosed herein are methods and compositions for rapidly identifying and ranking nucleases for specific cleavage of a target sequence.

公开(公告)号：US08921112B2

公开(公告)日：2014-12-30

申请号：US12286668

申请日：2008-10-01

申请人： Qihua C. Cai ,  Vipula K. Shukla ,  Joseph F. Petolino ,  Lisa W. Baker ,  Robbi J. Garrison ,  Ryan C. Blue ,  Jon C. Mitchell ,  Nicole L. Arnold ,  Sarah E. Worden ,  Jeffrey Miller ,  Fyodor Urnov

发明人： Qihua C. Cai ,  Vipula K. Shukla ,  Joseph F. Petolino ,  Lisa W. Baker ,  Robbi J. Garrison ,  Ryan C. Blue ,  Jon C. Mitchell ,  Nicole L. Arnold ,  Sarah E. Worden ,  Jeffrey Miller ,  Fyodor Urnov

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H5/10 ,  A01H1/08 ,  C12N5/10

CPC分类号： C07K14/4702 ,  C07K2319/81 ,  C12N15/8213 ,  C12N15/8243

摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.

摘要翻译： 本文公开了包含CCHC锌配位残基的锌指。 还描述了锌指蛋白和包含这些CCHC锌指的融合蛋白以及编码这些蛋白的多核苷酸。 还描述了使用这些蛋白质进行基因编辑和基因调控的方法。

公开(公告)号：US08524500B2

公开(公告)日：2013-09-03

申请号：US12804234

申请日：2010-07-16

申请人： Fyodor Urnov ,  Michael C. Holmes ,  Jeffrey C. Miller ,  Carl O. Pabo

发明人： Fyodor Urnov ,  Michael C. Holmes ,  Jeffrey C. Miller ,  Carl O. Pabo

IPC分类号： C12N15/00 ,  C12N15/87

CPC分类号： A61K35/12 ,  C07K14/43595 ,  C07K14/4717 ,  C07K14/715 ,  C07K2319/00 ,  C07K2319/09 ,  C07K2319/81 ,  C12N9/14 ,  C12N9/22 ,  C12N15/62 ,  C12N15/85 ,  C12N2800/80

摘要： Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.

摘要翻译： 本文公开了用于基因组序列的靶向切割，基因组序列的靶向改变以及基因组区域和与该基因组区域同源的外源多核苷酸之间的靶向重组的方法和组合物。 组合物包括包含切割结构域（或切割半结构域）和工程化锌指结构域的融合蛋白和编码它们的多核苷酸。 用于靶向切割的方法包括将这种融合蛋白或其编码的多核苷酸引入细胞。 用于靶向重组的方法另外包括将与基因组区域同源的外源多核苷酸引入包含所公开的融合蛋白的细胞中。

公开(公告)号：US08071370B2

公开(公告)日：2011-12-06

申请号：US12803927

申请日：2010-07-09

申请人： Alan P. Wolffe ,  Elizabeth J. Wolffe, legal representative ,  Trevor Collingwood ,  Philip D. Gregory ,  Andrew Snowden ,  Fyodor Urnov

发明人： Alan P. Wolffe ,  Trevor Collingwood ,  Philip D. Gregory ,  Andrew Snowden ,  Fyodor Urnov

IPC分类号： C07H17/00 ,  C07K14/00

CPC分类号： C12N15/1058 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/24 ,  C07K2319/43 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12N15/67 ,  C12Q1/6811 ,  G06F19/22 ,  G06F19/28

摘要： Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences.

摘要翻译： 提供了在细胞染色质感兴趣的区域内靶向修饰染色质结构的方法和组合物。 这些方法和组合物可用于促进需要外源分子进入染色体DNA序列的过程，例如转录和重组。

公开(公告)号：US20110207221A1

公开(公告)日：2011-08-25

申请号：US12931760

申请日：2011-02-09

申请人： Gregory J. Cost ,  Dmitry M. Guschin ,  Fyodor Urnov

发明人： Gregory J. Cost ,  Dmitry M. Guschin ,  Fyodor Urnov

IPC分类号： C12N15/00 ,  C07H21/00 ,  C12N15/13 ,  C12N15/52 ,  C12N15/18 ,  C12N15/16

CPC分类号： C12N15/902 ,  C07K2319/80 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/907

摘要： Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.

公开(公告)号：US20110136895A1

公开(公告)日：2011-06-09

申请号：US12927557

申请日：2010-11-17

申请人： Philip D. Gregory ,  Michael C. Holmes ,  Matthew C. Mendel ,  Xiangdong Meng ,  David Paschon ,  Andreas Reik ,  Fyodor Urnov

发明人： Philip D. Gregory ,  Michael C. Holmes ,  Matthew C. Mendel ,  Xiangdong Meng ,  David Paschon ,  Andreas Reik ,  Fyodor Urnov

IPC分类号： A61K31/7088 ,  C07K14/435 ,  C07H21/00 ,  C12N5/0783 ,  A61P35/00 ,  A61P31/12

CPC分类号： A61K38/1709 ,  A61K35/17 ,  A61P31/12 ,  A61P35/00 ,  C07K2319/71 ,  C12N9/22

摘要： Disclosed herein are methods and compositions for modulating expression of a PD1 gene.

摘要翻译： 本文公开了调节PD1基因表达的方法和组合物。

公开(公告)号：US20100323424A1

公开(公告)日：2010-12-23

申请号：US12803927

申请日：2010-07-09

申请人： Alan P. Wolffe ,  Elizabeth J. Wolffe ,  Trevor Collingwood ,  Philip D. Gregory ,  Andrew Snowden ,  Fyodor Urnov

发明人： Alan P. Wolffe ,  Elizabeth J. Wolffe ,  Trevor Collingwood ,  Philip D. Gregory ,  Andrew Snowden ,  Fyodor Urnov

IPC分类号： C12N9/14 ,  C12N9/00 ,  C12N9/10 ,  C12N9/78 ,  C07H21/00 ,  C12N5/07 ,  C12N5/10

CPC分类号： C12N15/1058 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/24 ,  C07K2319/43 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12N15/67 ,  C12Q1/6811 ,  G06F19/22 ,  G06F19/28

摘要： Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences.

公开(公告)号：US07217509B2

公开(公告)日：2007-05-15

申请号：US09844501

申请日：2001-04-27

申请人： Alan Wolffe ,  Fyodor Urnov

发明人： Alan Wolffe ,  Fyodor Urnov

IPC分类号： C12Q1/68

CPC分类号： C12N15/1058 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/24 ,  C07K2319/43 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12N15/67 ,  C12Q1/6811 ,  G06F19/22 ,  G06F19/28 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2522/101

摘要： Methods and compositions for the identification, isolation and characterization of regulatory DNA sequences in a cell of interest are provided. Also provided are libraries of regulatory sequences obtained according to the methods, and databases comprising collections of regulatory sequences for a particular cell of interest. In addition, various uses for the regulatory sequences so obtained, and uses for the databases of regulatory sequences, are provided. Also disclosed are computer systems and computer program products for utilizing the databases to conduct various genetic analyses, and uses of accessible regulatory sequences in the design of vectors bearing transgenes.

摘要翻译： 提供了用于鉴定，分离和鉴定感兴趣的细胞中调节DNA序列的方法和组合物。 还提供了根据该方法获得的调节序列的文库，以及包含特定感兴趣细胞的调节序列的集合的数据库。 此外，还提供了如此获得的调节序列的各种用途以及调节序列数据库的用途。 还公开了用于利用数据库进行各种遗传分析的计算机系统和计算机程序产品，以及在设计带有转基因的载体的设计中使用可及的调节序列。

公开(公告)号：US06777185B2

公开(公告)日：2004-08-17

申请号：US09925796

申请日：2001-08-09

申请人： Casey C. Case ,  Lei Zhang ,  Fyodor Urnov

发明人： Casey C. Case ,  Lei Zhang ,  Fyodor Urnov

IPC分类号： C12Q168

CPC分类号： G01N33/5005 ,  C12N15/1055 ,  C12N15/1079 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8243 ,  C12Q1/6897

摘要： The present invention provides methods of regulating gene expression using recombinant zinc finger proteins, for functional genomics and target validation applications.

摘要翻译： 本发明提供了使用重组锌指蛋白调节基因表达的方法，用于功能基因组学和靶标验证应用。