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    Sperm staining and sorting methods
    32.
    发明授权
    Sperm staining and sorting methods 有权
    精子染色和分选方法

    公开(公告)号：US09581587B2

    公开(公告)日：2017-02-28

    申请号：US14348194

    申请日：2012-09-28

    申请人： INGURAN, LLC

    发明人： Kenneth Michael Evans ,  Thomas B. Gilligan ,  Clara Gonzalez-Marin

    IPC分类号： G01N33/50 ,  G01N21/64 ,  C12N5/076

    CPC分类号： G01N33/5005 ,  C12N5/061 ,  G01N21/6428 ,  G01N2021/6432 ,  G01N2021/6441

    摘要： A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.

    摘要翻译： 公开了一种性别分类精子的方法。 精子可能被DNA选择性荧光染料染色，当激发时发出荧光，残留的淬灭染料会选择性地猝灭受精精子膜内的DNA选择性荧光染料发出的荧光，以及分裂增强染料。 然后将染色的精子分选成一个或多个性别富集的活的精子亚群。

    AGENT FOR IMPROVING SPERM-MOTILITY
    33.
    发明申请
    AGENT FOR IMPROVING SPERM-MOTILITY 审中-公开
    改善运动的代理

    公开(公告)号：US20160281057A1

    公开(公告)日：2016-09-29

    申请号：US15032446

    申请日：2014-10-30

    申请人： Naoki YAMASHITA ,  TOKYO METROPOLITAN GERIATRIC HOSPITAL AND INSTITUTE OF GERONTOLOGY

    发明人： Naoki Yamashita ,  Kumiko Nakata ,  Ikuroh Ohsawa

    IPC分类号： C12N5/076

    CPC分类号： C12N5/061 ,  A61K33/00 ,  C12N2500/02

    摘要： The present invention addresses the problem of providing an agent for improving sperm-motility, in particular, an agent for improving forward-sperm-motility, for use on reduced-motility sperm in male infertility treatment. Prepared is a gaseous agent for improving sperm-motility consisting of a gas that contains hydrogen molecules in the amount of 1% (v/v) or higher, for example, a gas that contains hydrogen molecules in an amount of between 45 and 55% (v/v), or a liquid agent for improving sperm-motility consisting of a liquid such as physiological saline, a culture solution, or a buffer solution containing hydrogen molecules in the amount of 1% or higher of the saturation solubility thereof, for example, a liquid in which hydrogen molecules have been dissolved by bubbling. The gaseous agent for improving sperm-motility can improve the motility of sperm by contacting hydrogen gas and either semen or a dilution thereof with each other in a gaseous phase. The liquid agent for improving sperm-motility can improve the motility of sperm when used as a washing solution or diluent for semen.

    摘要翻译： 本发明解决了在男性不孕症治疗中提供用于改善精子运动性的药剂，特别是用于改善前精子运动性的药剂用于减少精子活力的问题。 制备的是用于提高精子活力的气态剂，其由含有1％（v / v）以上的氢分子的气体组成，例如含有45〜55％的氢分子的气体， （v / v）或用于提高精液运动性的液体剂，其由例如生理盐水，培养溶液或含有其饱和溶解度的1％以上的氢分子的缓冲溶液组成的液体试剂用于 例如，通过鼓泡将氢分子溶解的液体。 用于提高精子活力的气态剂可以通过在气相中将氢气和精液或其稀释液彼此接触来改善精子的运动性。 用于提高精子活力的液体剂可以提高精子在作为精液的洗涤液或稀释剂时的运动性。

    Sperm diluent solution and method for artificial insemination using same
    34.
    发明授权
    Sperm diluent solution and method for artificial insemination using same 有权
    精子稀释液和人工授精方法使用相同

    公开(公告)号：US09439414B2

    公开(公告)日：2016-09-13

    申请号：US13378560

    申请日：2010-06-17

    申请人： Masayuki Shimada ,  Tetsuji Okazaki

    发明人： Masayuki Shimada ,  Tetsuji Okazaki

    IPC分类号： A01N1/02 ,  C12N5/076 ,  A61K35/12

    CPC分类号： A01N1/0221 ,  A61K35/12 ,  C12N5/061 ,  C12N2500/14 ,  C12N2517/10

    摘要： A sperm diluent of the present invention contains a chelating agent such as EDTA and/or, EGTA, which forms a complex with a calcium ion, in a base diluent. Further, the sperm diluent contains an immunosuppressive factor such as a steroid hormone and/or, a cytokine, which suppresses migration of leukocytes. By diluting frozen sperm with this sperm diluent followed by performing artificial insemination, death of the sperm before fertilization and phagocytosis of the sperm and embryos by leukocytes in the uterus can be suppressed, allowing enhancement of the conception rate and the implantation rate.

    摘要翻译： 本发明的精子稀释液在碱性稀释剂中含有螯合剂如EDTA和/或与钙离子形成络合物的EGTA。 此外，精子稀释液含有抑制白细胞迁移的免疫抑制因子，例如类固醇激素和/或细胞因子。 通过用精子稀释液稀释冷冻精子，然后进行人工授精，可以抑制子宫内受精前的精子死亡和白细胞对精子和胚胎的吞噬作用，从而提高受孕率和植入率。

    Compositions and methods for processing sperm
    35.
    发明授权
    Compositions and methods for processing sperm 有权

    公开(公告)号：US09347040B2

    公开(公告)日：2016-05-24

    申请号：US13844636

    申请日：2013-03-15

    申请人： INGURAN, LLC

    发明人： Richard Lenz ,  Juan Moreno ,  Ramakrishnan Vishwanath

    IPC分类号： A01N1/02 ,  C12N5/076 ,  G01N15/14

    CPC分类号： C12N5/0609 ,  A01N1/0226 ,  A01N1/0284 ,  C12N5/061 ,  C12N5/0612 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2502/04 ,  G01N15/1459 ,  G01N2015/149

    摘要： The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.

    Compositions and methods for processing sperm
    36.
    发明授权
    Compositions and methods for processing sperm 有权
    精子加工的组成和方法

    公开(公告)号：US09347038B2

    公开(公告)日：2016-05-24

    申请号：US13823843

    申请日：2012-06-01

    申请人： Richard Lenz ,  Juan Moreno ,  Ramakrishnan Vishwanath

    发明人： Richard Lenz ,  Juan Moreno ,  Ramakrishnan Vishwanath

    IPC分类号： A01N1/02 ,  C12N5/076 ,  G01N15/14

    CPC分类号： C12N5/0609 ,  A01N1/0226 ,  A01N1/0284 ,  C12N5/061 ,  C12N5/0612 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2502/04 ,  G01N15/1459 ,  G01N2015/149

    摘要： The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.

    摘要翻译： 本发明涉及用于处理加工精子的组合物和方法，包括新鲜收集的样品，作为新鲜样品运输的样品，被冷冻和解冻的样品，分为一个或多个亚种群的样品，以及另外处理或处理的样品 对细胞造成创伤。 创伤可以降低精子的活力，生育力，生存力和整体完整性，降低受精，产生胚胎和健康后代的能力。 本发明涉及可以添加到精子细胞样品中以减轻轻度和广泛精子细胞处理期间物理应激的创伤作用的新型化合物，在标准精子加工程序中使用化合物的方法，由 这些方法包括精子和胚胎，以及在动物的辅助生殖生物学技术中使用这些终产物的方法。

    SPERM CELL SEPARATION METHODS AND COMPOSITIONS CONTAINING APTAMERS OR NUCLEIC ACID SEQUENCES FOR USE THEREIN
    38.
    发明申请
    SPERM CELL SEPARATION METHODS AND COMPOSITIONS CONTAINING APTAMERS OR NUCLEIC ACID SEQUENCES FOR USE THEREIN 有权

    公开(公告)号：US20150203811A1

    公开(公告)日：2015-07-23

    申请号：US14472155

    申请日：2014-08-28

    申请人： BIOCERN, INC.

    发明人： DAVID OKSENBERG ,  SERGEY KRYLOV ,  MICHAEL MUSHEEV

    IPC分类号： C12N5/076 ,  C12N5/071

    CPC分类号： C12Q1/6811 ,  C12N5/061 ,  C12N5/0612 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/10 ,  C12Q1/6879

    摘要： The present invention provides an aptamer or pool of aptamers (nucleic acid sequences) that bind(s) to a target molecule on the surface, accessible from the surface or inside of a mammalian sperm cell and a method for producing the aptamers. The method comprises contacting a collection of different nucleic acid molecules with the target molecule under conditions favorable for binding between at least one of the nucleic acid molecules and the target molecule, to form at least one complex comprising the nucleic acid molecule bound to the target molecule, wherein each of the nucleic acid molecules comprises at least one segment of randomized nucleotide sequences. The complexes are then separated from the unbound nucleic acid molecules and unbound target molecules, and the bound nucleic acid molecule is recovered from the separated complex. The aptamers are used to separate sperm cells based upon sperm cell qualities, such as whether the cells contain X chromosomes or Y chromosomes.