公开(公告)号：US11752470B2

公开(公告)日：2023-09-12

申请号：US16315069

申请日：2017-08-23

Applicant: EMD Millipore Corporation

Inventor： Songhua Liu ,  Michael Lynch ,  Ryan Sylvia ,  Joseph Almasian ,  Salvatore Giglia ,  Luc Messier

IPC: B01D63/14 ,  B01D46/04 ,  B01D46/52 ,  B01D46/00

CPC classification number: B01D63/14 ,  B01D46/00 ,  B01D46/04 ,  B01D46/522 ,  B01D2201/122 ,  B01D2313/20

Abstract: A scaling tool for replicating filtration characteristics of a large-scale filtration device, the scaling tool comprising a housing having a fluid inlet and a fluid outlet, and a filter receiving region in fluid communication with the fluid inlet and the fluid outlet and configured to contain a membrane comprising one or more pleats, the filter receiving region being defined by a rigid wall framework configured to hold the one or more pleats of the membrane and compress the pleats in an amount effective to replicate the filtration performance of the large-scale filtration device. Also disclosed is a methodology for configuring a filter receiving region in a scaling tool.

公开(公告)号：US11732003B2

公开(公告)日：2023-08-22

申请号：US16610616

申请日：2018-04-18

Applicant: EMD Millipore Corporation

Inventor： Ronald Tuccelli ,  John Caulmare ,  Luc Messier ,  Melissa Holstein ,  Christopher Gillespie ,  Roger Hill

IPC: A61L2/00 ,  A61L2/26 ,  B01D15/24 ,  B01L3/00 ,  C12N7/00 ,  C07K1/22 ,  B01D15/36 ,  B01D15/38

CPC classification number: C07K1/22 ,  A61L2/0088 ,  A61L2/26 ,  B01D15/245 ,  B01D15/361 ,  B01D15/3809 ,  B01L3/502 ,  C12N7/00 ,  A61L2202/21 ,  B01L2400/0481 ,  B01L2400/082 ,  C12N2740/10063 ,  C12N2740/10064 ,  C12N2795/00063 ,  C12N2795/00064

Abstract: Methods of maintaining narrow residence time distributions in continuous flow systems, particularly applicable to virus inactivation such as during a protein purification process. Fluid sample is introduced into an axial flow channel and caused to flow therein in discrete packets or zones to minimize residence time distribution and axial dispersion. Embodiments described herein obviate or minimize the need for using large tanks or reservoirs for performing virus inactivation during a protein purification process; reduce the overall time required for virus inactivation, and/or reduce the overall physical space required to perform the virus inactivation operation during a protein purification process, which in turn reduces the overall footprint for the purification process.

公开(公告)号：US11712641B2

公开(公告)日：2023-08-01

申请号：US16734641

申请日：2020-01-06

Applicant: EMD Millipore Corporation

Inventor： Stephane Olivier ,  Didier Metz

IPC: B01D27/04 ,  G01N1/40

CPC classification number: B01D27/04 ,  G01N1/4077 ,  G01N2001/4088

Abstract: The invention relates to the filtering of liquid samples, preferably in a laboratory environment, for example for collecting micro organisms from the liquid sample for subsequent testing. The invention provides a method for filtering a liquid sample which comprises providing a blank of a deformable sheet material and a filtration medium, deforming the blank to form a funnel extending above and about the filtration medium, introducing the liquid sample to be filtered into the so formed funnel, and filtering the liquid sample from the funnel through the filtration medium, for example to a downstream receptacle. The method is accordingly based on the concept that the funnel is not or at least not completely preformed but provided in a form of a blank of a deformable material sheet which is deformed so as to form the funnel at the point and time of use during filtration.

公开(公告)号：USD980235S1

公开(公告)日：2023-03-07

申请号：US29758164

申请日：2020-11-12

Applicant: Sigma-Aldrich Co. LLC ,  EMD Millipore Corporation

Designer： Sarah Trice ,  Lindsey Rickershauser ,  Xiang Wu ,  Churl Oh ,  Nadia Brugger ,  Emma Gardener

公开(公告)号：US20230002711A1

公开(公告)日：2023-01-05

申请号：US17773858

申请日：2020-11-03

Applicant: EMD Millipore Corporation

Inventor： Joseph Cianciolo

IPC: C12M1/00

Abstract: Some embodiments of the present disclosure comprise a flow diverter for use in a biocontainer or bioreactor, comprising a shoulder having a first surface and a second surface opposite the first surface, a conduit extending from the first surface of the shoulder, an inlet at a first end of the conduit, wherein the first end comprises a connector, an outlet formed within the second surface of the shoulder in fluid communication with the inlet at an end opposite the first end of the conduit and a hood adjacent to the outlet, wherein a fluid diverter is capable of directing a fluid down a sidewall of the biocontainer or bioreactor, attenuating a splashing or foaming condition.

公开(公告)号：US20220387917A1

公开(公告)日：2022-12-08

申请号：US17819969

申请日：2022-08-16

Applicant: EMD Millipore Corporation

Inventor： Maybelle Woo ,  David Yavorsky ,  John Amara ,  Nripen Singh ,  Kwok-Shun Cheng

IPC: B01D39/16 ,  B01D29/66

Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.

公开(公告)号：US11511281B2

公开(公告)日：2022-11-29

申请号：US16878522

申请日：2020-05-19

Applicant: EMD Millipore Corporation

Inventor： Joseph Muldoon ,  Rene Reinbigler

IPC: B01L3/00 ,  A61B5/15 ,  G01N1/14 ,  A61M5/178 ,  A61M5/31 ,  A61M5/315 ,  A61B5/153

Abstract: A fluid transfer device includes a syringe barrel having a chamber, a first plunger slidably movable inside the chamber, and a second plunger slidably movable inside the chamber. The distal end portion of the first plunger is engageable with the proximal end portion of the second plunger such that when the distal end portion of the first plunger and the proximal end portion of the second plunger are engaged, the second plunger is movable by the first plunger. A check valve may be incorporated into the distal end portion of the second plunger to allow a fluid to pass therethrough in a direction towards the proximal end portion of the second plunger and prevent a fluid to pass therethrough in a reverse direction. A fluid transfer assembly and a sampling method are also described.

公开(公告)号：US11410752B2

公开(公告)日：2022-08-09

申请号：US16772293

申请日：2019-07-30

Applicant: EMD Millipore Corporation ,  Sigma-Aldrich Co. LLC

Inventor： Sarah Trice ,  Tim Knehans ,  Maciej Wojcikowski

IPC: G06F21/60 ,  G16C60/00 ,  G06F21/62 ,  G16C20/90

Abstract: A system, software application and method that allows a customer to protect their proprietary database of compounds and substances while utilizing a retrosynthesis software application is disclosed. The customer's proprietary database is encrypted prior to being provided to the retrosynthesis system. This encrypted is performed using a hash and optionally a salt. The retrosynthesis algorithm then creates synthons as is traditionally done. However, after their creation, the synthons are hashed so that they may be compared to the entries in the customer's proprietary database. In this way, the actual contents of the customer's database are never made available to the retrosynthesis system or software application.

公开(公告)号：US11278827B2

公开(公告)日：2022-03-22

申请号：US16427942

申请日：2019-05-31

Applicant: EMD Millipore Corporation

Inventor： Jonathan Steen ,  Mikhail Kozlov ,  Herbert Lutz ,  Joseph Parrella ,  Elizabeth M. Goodrich ,  Bala Raghunath

IPC: B01D29/90 ,  B01D61/14 ,  B01D63/04 ,  B01D63/12 ,  B01D29/52 ,  B01D29/60

Abstract: Methods of filtering a liquid feed are disclosed. In one version, the method comprises passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system. In another version, the method of filtering a liquid feed, comprises passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

公开(公告)号：US11236125B2

公开(公告)日：2022-02-01

申请号：US16561453

申请日：2019-09-05

Applicant: EMD Millipore Corporation

Inventor： Matthew T. Stone ,  John P. Amara

IPC: C07K1/20 ,  C07K1/18 ,  C07K1/36 ,  B01D15/38 ,  B01D63/02

Abstract: The present invention refers to new species of an ion exchange adsorber which is suitable for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies. The invention especially refers to a process for purifying biological samples by separating biomolecules of interest and impurities, comprising steps of contacting a sample with said chromatography media consisting of fibers, said fibers having imparted thereon functionality enabling ion exchange chromatography and/or hydrophobic interaction.