公开(公告)号：US20190153454A1

公开(公告)日：2019-05-23

申请号：US16194943

申请日：2018-11-19

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya

IPC: C12N15/52 ,  C12N15/70 ,  C12N9/88 ,  C12P13/00 ,  C12N9/10

Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.

公开(公告)号：US20190127764A1

公开(公告)日：2019-05-02

申请号：US16004285

申请日：2018-06-08

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/04 ,  C12N15/70 ,  C12N15/52 ,  C12P5/02

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.

公开(公告)号：US20190112247A1

公开(公告)日：2019-04-18

申请号：US15975630

申请日：2018-05-09

Applicant: Genomatica, Inc.

Inventor： Warren Clark ,  Michael Japs ,  Mark J. Burk

IPC: C07C29/76 ,  C12P7/18

Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.

公开(公告)号：US20190085360A1

公开(公告)日：2019-03-21

申请号：US15971977

申请日：2018-05-04

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Robin E. Osterhout

IPC: C12P5/02 ,  C12N15/52 ,  C12N9/88 ,  C12N9/12 ,  C12P5/00

Abstract: The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene.

公开(公告)号：US10208320B2

公开(公告)日：2019-02-19

申请号：US14987489

申请日：2016-01-04

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Anthony P. Burgard ,  Priti Pharkya

IPC: C12P7/04 ,  C12N15/52 ,  C12N9/02 ,  C12P7/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88

Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：US10150976B2

公开(公告)日：2018-12-11

申请号：US14107832

申请日：2013-12-16

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P13/02 ,  C12P7/24 ,  C12P19/02 ,  C12P7/44 ,  C12P13/00 ,  C12N9/04 ,  C08G63/78 ,  C08G73/02 ,  C08G69/16 ,  C08G69/08 ,  C12N15/52 ,  A23L29/00

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US10081587B2

公开(公告)日：2018-09-25

申请号：US15182245

申请日：2016-06-14

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

IPC: C07C51/353 ,  C12P7/46 ,  C07C51/38 ,  C07C67/32 ,  C07C57/04 ,  C07C67/08 ,  C07C67/333 ,  C12P7/40 ,  C12P7/62

CPC classification number: C07C51/353 ,  C07C51/38 ,  C07C57/04 ,  C07C67/08 ,  C07C67/32 ,  C07C67/333 ,  C12P7/40 ,  C12P7/46 ,  C12P7/62 ,  Y02P20/125 ,  C07C69/60 ,  C07C69/54

Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

公开(公告)号：US10000758B2

公开(公告)日：2018-06-19

申请号：US15133150

申请日：2016-04-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N15/52 ,  C12P7/42 ,  C08F120/06 ,  C08G63/664 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12P7/62

CPC classification number: C12N15/52 ,  C08F120/06 ,  C08G63/664 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12Y101/01244 ,  C12Y105/0102 ,  C12Y201/0109

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

公开(公告)号：US09873652B2

公开(公告)日：2018-01-23

申请号：US14776517

申请日：2014-03-14

Applicant: Genomatica, Inc.

Inventor： SVB Janardhan Garikipati ,  Michael Japs ,  Ishmael M. Sonico

IPC: C07C29/80 ,  C07C29/74 ,  B01D3/14 ,  C07C29/90 ,  B01D1/22 ,  B01D3/00 ,  C07C29/00 ,  C07C31/20 ,  C12P7/18

CPC classification number: C07C29/80 ,  B01D1/22 ,  B01D3/002 ,  B01D3/143 ,  B01D3/145 ,  C07C29/00 ,  C07C29/74 ,  C07C29/90 ,  C07C31/207 ,  C12P7/18 ,  C07C33/046

Abstract: A process of purifying 1,4-butanediol (1,4-BDO) from a fermentation broth including separating solid materials, salts and water, and subjecting the resulting material to a two, three or four column distillation system, that can include a wiped film evaporator to produce a purified 1,4-butanediol product.

公开(公告)号：US20170362565A1

公开(公告)日：2017-12-21

申请号：US15585078

申请日：2017-05-02

Applicant: Genomatica, Inc.

Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Stephen J. VAN DIEN ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  John D. TRAWICK ,  Michael P. KUCHINSKAS ,  Brian STEER

IPC: C12N1/20 ,  C12P7/18 ,  C12N15/63 ,  C12N15/52 ,  C12N9/18 ,  C07C31/20 ,  C08G63/181 ,  C08G63/16 ,  C08G63/06 ,  C08G18/32 ,  C08G18/28 ,  C07C59/01 ,  C12P7/42 ,  C08G69/02

CPC classification number: C12N1/20 ,  C07C31/207 ,  C07C59/01 ,  C08G18/284 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C12P7/18 ,  C12P7/42 ,  C12Y301/01 ,  Y02P20/52

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.