公开(公告)号：US5869315A

公开(公告)日：1999-02-09

申请号：US573890

申请日：1995-12-18

Applicant: Robert V. Talanian ,  John A. Mankovich ,  Tariq Ghayur ,  Catherine R. Ferenz

Inventor： Robert V. Talanian ,  John A. Mankovich ,  Tariq Ghayur ,  Catherine R. Ferenz

IPC: C12N9/64 ,  C07H21/04 ,  C07K1/00 ,  C12N9/99

CPC classification number: C12N9/6475

Abstract: Modified forms of human interleukin-1.beta. converting enzyme (ICE) that display proteolytic activity and, furthermore, have increased stability compared to unmodified human ICE are disclosed. Nucleic acid molecules encoding a modified p10 subunit of ICE, and recombinant vectors and host cells incorporating such nucleic acid molecules, are also disclosed. A modified ICE protein of the invention can be used to cleave proteolytically ICE substrates and to identify modulators of ICE activity in screening assays. Moreover, due to its enhanced stability, the modified ICE of the invention is particularly suitable for use in the preparation of ICE crystals for X-ray crystallography.

Abstract translation: 公开了与未修饰的人类ICE相比显示出蛋白水解活性的人白细胞介素-1β转换酶（ICE）的修饰形式，此外具有增加的稳定性。 还公开了编码ICE的修饰的p10亚基的核酸分子，以及掺入了这种核酸分子的重组载体和宿主细胞。 本发明的修饰的ICE蛋白质可用于切割蛋白水解的ICE底物并鉴定筛选测定中ICE活性的调节剂。 此外，由于其稳定性的提高，本发明的改性ICE特别适用于制备用于X射线晶体学的ICE晶体。

公开(公告)号：US20150017168A1

公开(公告)日：2015-01-15

申请号：US14332087

申请日：2014-07-15

Applicant: Tariq Ghayur ,  Junjian Liu ,  Gillian A. Kingsbury ,  Edward B. Reilly ,  Susan E. Morgan-Lappe

Inventor： Tariq Ghayur ,  Junjian Liu ,  Gillian A. Kingsbury ,  Edward B. Reilly ,  Susan E. Morgan-Lappe

IPC: C07K16/28 ,  A61K45/06 ,  C07K16/22 ,  A61K47/48 ,  A61K39/395 ,  C07K16/40

CPC classification number: C07K16/2863 ,  A61K39/3955 ,  A61K39/39558 ,  A61K45/06 ,  A61K47/6879 ,  A61K2039/505 ,  C07K16/22 ,  C07K16/28 ,  C07K16/2809 ,  C07K16/40 ,  C07K16/468 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/00 ,  G01N33/74 ,  G01N2333/71 ,  Y02A50/386 ,  Y02A50/41 ,  Y02A50/412

Abstract: The present invention relates to engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention, diagnosis, and/or treatment of disease.

公开(公告)号：US08822645B2

公开(公告)日：2014-09-02

申请号：US12499652

申请日：2009-07-08

Applicant: Tariq Ghayur ,  Jijie Gu ,  Peter C. Isakson

Inventor： Tariq Ghayur ,  Jijie Gu ,  Peter C. Isakson

IPC: C07K16/00 ,  C12P21/08 ,  A61K39/395 ,  A61K39/40 ,  C07H21/04 ,  G01N33/53 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C12N5/00 ,  C12N5/16 ,  C07K1/00

CPC classification number: C07K16/468 ,  A61K38/00 ,  A61K45/06 ,  A61K47/6879 ,  A61K51/109 ,  C07K16/22 ,  C07K16/24 ,  C07K16/26 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/44 ,  C07K2317/31 ,  C07K2317/64 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2317/734 ,  G01N33/6869 ,  G01N33/74 ,  G01N33/88 ,  G01N2333/4709 ,  G01N2333/48 ,  G01N2333/525 ,  G01N2333/5443 ,  G01N2333/545 ,  G01N2333/7155 ,  Y02A50/385 ,  Y02A50/401 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/463 ,  Y02A50/478 ,  Y02A50/481

Abstract: The present invention relates to engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention, diagnosis, and/or treatment of disease.

Abstract translation: 本发明涉及工程化的多价和多特异性结合蛋白，制备方法，具体涉及用于预防，诊断和/或治疗疾病的用途。

公开(公告)号：US08735546B2

公开(公告)日：2014-05-27

申请号：US13196138

申请日：2011-08-02

Applicant: Tariq Ghayur ,  Junjian Liu ,  Peter C. Isakson

Inventor： Tariq Ghayur ,  Junjian Liu ,  Peter C. Isakson

IPC: C07K16/00 ,  C12P21/08 ,  C07H21/04 ,  C12P21/06 ,  C12N5/02 ,  C12N5/06 ,  C12N1/20

CPC classification number: C07K16/244 ,  A61K39/3955 ,  A61K45/06 ,  A61K47/6845 ,  A61K49/00 ,  A61K51/1021 ,  C07K16/241 ,  C07K16/245 ,  C07K16/468 ,  C07K2317/35 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2317/94 ,  G01N33/53 ,  G01N33/56966 ,  G01N33/6857 ,  G01N33/6869 ,  G01N2333/54 ,  G01N2333/545 ,  G01N2800/24 ,  G01N2800/7028

Abstract: Engineered multivalent and multispecific binding proteins, methods of making, and their uses in the prevention, diagnosis, and/or treatment of disease are provided.

Abstract translation: 提供了工程化的多价和多特异性结合蛋白，制备方法及其在预防，诊断和/或治疗疾病中的用途。

公开(公告)号：US20140134171A1

公开(公告)日：2014-05-15

申请号：US14056116

申请日：2013-10-17

Applicant: Tariq Ghayur ,  Junjian Liu ,  Sharmila Manoj ,  Susan E. Brophy

Inventor： Tariq Ghayur ,  Junjian Liu ,  Sharmila Manoj ,  Susan E. Brophy

IPC: C07K16/46 ,  C07K16/30 ,  C07K16/44

CPC classification number: C07K16/468 ,  A61K45/06 ,  C07K16/00 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/2863 ,  C07K16/2887 ,  C07K16/30 ,  C07K16/44 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/515 ,  C07K2317/64 ,  C07K2317/732 ,  C07K2317/92

Abstract: The present invention relates to engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention, diagnosis, and/or treatment of disease.

Abstract translation: 本发明涉及工程化的多价和多特异性结合蛋白，制备方法，具体涉及用于预防，诊断和/或治疗疾病的用途。

公开(公告)号：US08722855B2

公开(公告)日：2014-05-13

申请号：US12914614

申请日：2010-10-28

Applicant: Tariq Ghayur ,  Jochen G. Salfeld ,  Michael J. McPherson ,  Maria C. Harris ,  Junjian Liu ,  Peter C. Isakson ,  Jijie Gu

Inventor： Tariq Ghayur ,  Jochen G. Salfeld ,  Michael J. McPherson ,  Maria C. Harris ,  Junjian Liu ,  Peter C. Isakson ,  Jijie Gu

IPC: C07K16/00 ,  C12P21/08 ,  C07H21/04 ,  C12P21/06 ,  C12N5/02 ,  C12N5/06 ,  C12N1/20 ,  C07K1/00

CPC classification number: C07K16/241 ,  C07K16/22 ,  C07K16/2803 ,  C07K16/2875 ,  C07K16/44 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/73

Abstract: The present invention relates to engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention, diagnosis, and/or treatment of disease.

公开(公告)号：US20140100359A1

公开(公告)日：2014-04-10

申请号：US14023336

申请日：2013-09-10

Applicant: Chengbin WU ,  Tariq GHAYUR ,  Richard W. DIXON ,  Jochen G. SALFELD

Inventor： Chengbin WU ,  Tariq GHAYUR ,  Richard W. DIXON ,  Jochen G. SALFELD

IPC: C07K16/28 ,  C07K16/22 ,  C07K16/24

CPC classification number: C07K16/468 ,  A61K39/3955 ,  A61K45/06 ,  A61K47/42 ,  A61K47/6803 ,  A61K51/1093 ,  A61K2039/505 ,  C07K16/22 ,  C07K16/24 ,  C07K16/241 ,  C07K16/244 ,  C07K16/245 ,  C07K16/2809 ,  C07K16/2887 ,  C07K16/2896 ,  C07K16/40 ,  C07K16/46 ,  C07K16/467 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/51 ,  C07K2317/522 ,  C07K2317/56 ,  C07K2317/64 ,  C07K2317/76 ,  Y02A50/386 ,  Y02A50/41 ,  Y02A50/412 ,  Y02A50/58

Abstract: The present invention relates to engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention and/or treatment of acute and chronic inflammatory and other diseases.

公开(公告)号：US20130171059A1

公开(公告)日：2013-07-04

申请号：US13729564

申请日：2012-12-28

Applicant: Tariq GHAYUR ,  Junjian Liu

Inventor： Tariq GHAYUR ,  Junjian Liu

IPC: C07K16/24 ,  A61K45/06 ,  A61K39/395

CPC classification number: C07K16/241 ,  A61K39/3955 ,  A61K45/06 ,  C07K16/18 ,  C07K16/22 ,  C07K16/26 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/52 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2319/00

Abstract: Multivalent and multispecific binding proteins, methods of making, and their uses in the diagnosis, prevention, and/or treatment diseases are provided.

Abstract translation: 提供了多价和多特异性结合蛋白，制备方法及其在诊断，预防和/或治疗疾病中的用途。

公开(公告)号：US20120230911A1

公开(公告)日：2012-09-13

申请号：US13314878

申请日：2011-12-08

Applicant: Chung-ming HSIEH ,  Carrie L. GOOGREAU ,  Sahana BOSE ,  Achim MOELLER ,  Tariq GHAYUR

Inventor： Chung-ming HSIEH ,  Carrie L. GOOGREAU ,  Sahana BOSE ,  Achim MOELLER ,  Tariq GHAYUR

IPC: A61K51/10 ,  C12P21/00 ,  C12N9/96 ,  C12N5/10 ,  C12N1/15 ,  C12N1/11 ,  C12N15/63 ,  G01N33/566 ,  C07K16/24 ,  C07H21/04 ,  A61P21/02 ,  A61P29/00 ,  A61P25/20 ,  A61P31/00 ,  A61P37/02 ,  A61P37/06 ,  A61P5/00 ,  A61P25/24 ,  A61P25/18 ,  A61P11/06 ,  A61P1/00 ,  A61P17/06 ,  A61P19/02 ,  A61P25/00 ,  A61P3/10 ,  A61P37/08 ,  A61P11/00 ,  A61P17/00 ,  A61P1/16 ,  A61P35/00 ,  A61P31/12 ,  A61P31/04 ,  A61P33/00 ,  A61K39/395

CPC classification number: C07K16/241 ,  A61K39/3955 ,  A61K45/06 ,  A61K47/48546 ,  A61K47/6845 ,  A61K49/0058 ,  A61K51/1021 ,  A61K2039/505 ,  A61K2039/54 ,  A61K2039/545 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2317/624 ,  C07K2317/626 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/30 ,  G01N33/6863

Abstract: TNF-α binding proteins, including chimeric, CDR-grafted, and humanized antibodies that bind TNF-α are provided. Binding proteins have high affinity for TNF-α and neutralize TNF-α activity. A binding protein can be a full-length antibody or a TNF-α-binding portion thereof. Methods of making and methods of using the binding proteins are also described. The TNF-α binding proteins are useful for detecting TNF-α and for inhibiting TNF-α activity, including in a human subject suffering from a disease or disorder in which TNF-α activity is detrimental.

Abstract translation: 提供TNF-α结合蛋白，包括结合TNF-α的嵌合，CDR移植和人源化抗体。 结合蛋白对TNF-α具有高亲和力，并中和TNF-α活性。 结合蛋白可以是全长抗体或其TNF-α结合部分。 还描述了制备方法和使用结合蛋白的方法。 TNF-α结合蛋白可用于检测TNF-α和抑制TNF-α活性，包括在患有TNF-α活性有害的疾病或病症的人类受试者中。

公开(公告)号：US20110250130A1

公开(公告)日：2011-10-13

申请号：US13081212

申请日：2011-04-06

Applicant: Lorenzo Benatuil ,  Tariq Ghayur ,  Carrie L. Goodreau ,  Peter C. Isakson ,  Jochen Salfeld

Inventor： Lorenzo Benatuil ,  Tariq Ghayur ,  Carrie L. Goodreau ,  Peter C. Isakson ,  Jochen Salfeld

IPC: A61K51/10 ,  A61K39/395 ,  C07H21/04 ,  A61K38/20 ,  A61P43/00 ,  A61P37/04 ,  A61P35/00 ,  A61P29/00 ,  A61P23/00 ,  A61P25/24 ,  A61P25/18 ,  A61P1/00 ,  A61P37/08 ,  A61P33/00 ,  A61P31/00 ,  A61P25/04 ,  C12N15/63 ,  C12N1/00 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/11 ,  C12P21/02 ,  G01N33/566 ,  C07K16/18

CPC classification number: C07K16/241 ,  A61K47/6845 ,  A61K51/1021 ,  A61K2039/505 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/6863 ,  G01N2333/7151 ,  Y02A50/386 ,  Y02A50/41 ,  Y02A50/412 ,  Y02A50/58

Abstract: Isolated binding proteins, e.g., antibodies or antigen binding portions thereof, which bind to tumor necrosis factor-alpha (TNF-α), e.g., human TNF-α, and related antibody-based compositions and molecules are disclosed. Also disclosed are pharmaceutical compositions comprising the antibodies, as well as therapeutic and diagnostic methods for using the antibodies.

Abstract translation: 公开了与肿瘤坏死因子-α（TNF-α）结合的分离的结合蛋白，例如结合肿瘤坏死因子-α（TNF-α）的抗体或其抗原结合部分以及相关的基于抗体的组合物和分子。 还公开了包含抗体的药物组合物，以及使用抗体的治疗和诊断方法。