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    Recombinant poxvirus host range selection system
    46.
    发明授权
    Recombinant poxvirus host range selection system 失效
    重组痘病毒宿主范围选择系统

    公开(公告)号：US5225336A

    公开(公告)日：1993-07-06

    申请号：US902428

    申请日：1992-06-23

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： A61K39/00 ,  C07K14/03 ,  C07K14/145 ,  C12N7/01 ,  C12N15/863

    CPC分类号： C12N15/86 ,  C07K14/005 ,  A61K39/00 ,  C12N2710/16722 ,  C12N2710/24143 ,  C12N2760/20122

    摘要： What is described is a modified recombinant virus for expressing a gene product in a host. The modified recombinant virus has host range genes deleted therefrom so that the virus has restricted replication in the host. The modified recombinant virus also contains DNA which codes for and expresses the gene product in the host even with restricted replication of the virus in the host. The modified recombinant virus is used in a method for expressing a gene product in a host or in a cell cultured in vitro, and in a vaccine for inducing an immunological response in a host inoculated with the vaccine. What is also described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conThis invention was made with Government support under contract DAMD17-85-C-5232 awarded by the Department of the Army. The Government has certain rights in this invention.

    摘要翻译： 描述的是用于在宿主中表达基因产物的修饰的重组病毒。 修饰的重组病毒具有从其中缺失的宿主范围基因，使得病毒在宿主中具有限制性的复制。 修饰的重组病毒还含有编码并在宿主中表达基因产物的DNA，甚至在宿主中病毒的复制受限。 修饰的重组病毒用于在宿主或体外培养的细胞中表达基因产物的方法，以及用于在接种疫苗的宿主中诱导免疫应答的疫苗。 还描述了在痘病毒，特别是痘苗病毒中克隆和表达开放阅读框的选择系统。 选择系统基于痘病毒的条件致死突变体（宿主范围）。 产生痘苗病毒的缺失/重组突变体，其能够在原代小鸡胚胎成纤维细胞和两种猴细胞系（BSC-40或VERO）上进行染色，但在人细胞系MRC-5中复制缺陷。 将宿主范围基因插入缺失/重组体中恢复MRC-5细胞生长能力。 构建了一系列质粒，当与缺失/重组重组并在MRC-5细胞上进行生长时，允许快速单步克隆和任何开放阅读框的表达。

    Recombinant poxvirus internal cores
    47.
    发明授权
    Recombinant poxvirus internal cores 失效
    重组POXVIRUS内部线

    公开(公告)号：US5204243A

    公开(公告)日：1993-04-20

    申请号：US480097

    申请日：1990-02-14

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： A61K39/275 ,  A61K39/295 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N15/09 ,  C12N15/863 ,  C12P21/00 ,  C12R1/91 ,  C12R1/92

    CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2710/24143

    摘要： What is described is a modified recombinant poxvirus, such as vaccinia virus, fowlpox virus and canarypox virus, having an internal core containing DNA from a non-pox source in a nonessential region of the poxvirus genome. The recombinant poxvirus is modified by disassociating the internal core from outer membranes of the poxvirus. The DNA codes for and expresses a gene product in a cell cultured in vitro and in a host in vivo without productive replication of the virus in the cell or in the host. What is also described is a vaccine containing the modified recombinant poxvirus for inducing an immunological response in a host inoculated with the vaccine.

    摘要翻译： 描述的是修饰的重组痘病毒，例如痘苗病毒，禽痘病毒和金丝雀痘病毒，其内含核心含有来自痘病毒基因组非必需区域中非痘源的DNA。 通过将内部核心与痘病毒的外膜分离来修饰重组痘病毒。 该DNA编码并表达体外培养的细胞中的基因产物，并在体内宿主中表达基因产物，而在细胞或宿主中不产生病毒的复制。 还描述了含有用于在接种疫苗的宿主中诱导免疫应答的修饰重组痘病毒的疫苗。

    Recombinant poxvirus host range selection system
    49.
    发明授权
    Recombinant poxvirus host range selection system 失效
    重建POXVIRUS主机范围选择系统

    公开(公告)号：US5155020A

    公开(公告)日：1992-10-13

    申请号：US320471

    申请日：1989-03-08

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： A61K39/00 ,  C07K14/03 ,  C07K14/145 ,  C12N15/39 ,  C12N15/863

    CPC分类号： C12N15/86 ,  C07K14/005 ,  A61K39/00 ,  C12N2710/16722 ,  C12N2710/24143 ,  C12N2760/20122

    摘要： What is described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conditional lethal mutant (host range) of poxviruses. A deletion/recombinant mutant of the vaccinia virus was generated which is capable of plaquing on primary chick embryo fibroblasts and two monkey cell lines (BSC-40 or VERO) but is defective in replication in the human cell line MRC-5. Insertion of the host range gene into the deletion/recombinant restores the ability for growth on MCR-5 cells. A series of plasmids were constructed which allow for the rapid single-step cloning and expression of any open reading frame when recombined with the deletion/recombinant and scored for growth on MCR-5 cells.

    摘要翻译： 描述的是在痘病毒，特别是痘苗病毒中克隆和表达开放阅读框的选择系统。 选择系统基于痘病毒的条件致死突变体（宿主范围）。 产生痘苗病毒的缺失/重组突变体，其能够在原代小鸡胚胎成纤维细胞和两种猴细胞系（BSC-40或VERO）上进行染色，但是在人细胞系MRC-5中的复制缺陷。 将宿主范围基因插入缺失/重组中恢复MCR-5细胞上的生长能力。 构建了一系列质粒，当与缺失/重组重组并在MCR-5细胞上进行生长时，允许快速单步克隆和任何开放阅读框的表达。

    POXVIRUS-PLASMODIUM RECOMBINANTS, COMPOSITIONS CONTAINING SUCH RECOMBINANTS, USES THEREOF, AND METHODS OF MAKING AND USING SAME
    50.
    发明申请
    POXVIRUS-PLASMODIUM RECOMBINANTS, COMPOSITIONS CONTAINING SUCH RECOMBINANTS, USES THEREOF, AND METHODS OF MAKING AND USING SAME 审中-公开
    POXVIRUS-PLASMODIUM重组体，包含这些重组体的组合物，其用途及其制备方法和使用方法

    公开(公告)号：US20150191704A1

    公开(公告)日：2015-07-09

    申请号：US14576578

    申请日：2014-12-19

    申请人： Enzo Paoletti ,  Randall L. Weinberg ,  Scott J. Goebel

    发明人： Enzo Paoletti ,  Randall L. Weinberg ,  Scott J. Goebel

    IPC分类号： C12N7/00 ,  A61K39/275

    CPC分类号： C12N7/00 ,  A61K39/015 ,  A61K39/275 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2710/24021 ,  C12N2710/24034 ,  C12N2710/24122 ,  C12N2710/24134 ,  C12N2710/24143 ,  Y02A50/412

    摘要： The invention provides a recombinant or synthetic or engineered or non-naturally occurring poxvirus that contains and expresses DNA encoding a heterologous or exogenous antigen, epitope or immunogen and Flagellin or an operable binding portion thereof. The poxvirus can contain or be engineered to contain and express vaccinia host range gene K1L. The poxvirus can be attenuated as to mammals, e.g., NYVAC, NYVAC.1, NYVAC.2, avipox, canarypox, fowlpox, ALVAC, TROVAC, MVA, or MVA-BN. The invention also provides methods for inducing an immunological response involving the poxvirus, and compositions containing the poxvirus. The antigen, epitope or immunogen that the poxvirus expresses can be at least one Plasmodium antigen. The Plasmodium antigen(s), epitope(s) or immunogen(s) can be SERA, ABRA, Pfhsp70, AMA-1, Pfs25, Pfs16, CSP, PfSSP2, LSA-1 repeatless, MSA-1, AMA-1 or combination(s) thereof. Advantageously the poxvirus contains DNA coding for and expresses Plasmodium antigen(s) CSP, PfSSP2, LSA-1-repeatless, MSA-1, SERA, AMA-1 and Pfs25. Also, advantageously, the poxvirus is a NYVAC poxvirus. The invention thus also provides an anti-malarial immunogenic or immunological compositions comprising the poxvirus, and methods for inducing an immunogenic or immunological response against malaria or Plasmodium in a mammal comprising administering to the mammal the poxvirus or an immunological or immunogenic composition containing the poxvirus. The mammal can be a human.

    摘要翻译： 本发明提供重组或合成或工程化或非天然存在的痘病毒，其含有和表达编码异源或外源抗原，表位或免疫原和鞭毛蛋白或其可操作结合部分的DNA。 痘病毒可以包含或被设计成含有和表达痘苗宿主范围基因K1L。 痘病毒可以减毒哺乳动物，例如NYVAC，NYVAC.1，NYVAC.2，avipox，金丝雀，禽痘，ALVAC，TROVAC，MVA或MVA-BN。 本发明还提供诱导涉及痘病毒的免疫应答的方法和含有痘病毒的组合物。 痘病毒表达的抗原，表位或免疫原可以是至少一种疟原虫抗原。 疟原虫抗原，表位或免疫原可以是SERA，ABRA，Pfhsp70，AMA-1，Pfs25，Pfs16，CSP，PfSSP2，LSA-1无重复，MSA-1，AMA-1或组合 （s）。 有利的是，痘病毒含有编码并表达疟原虫抗原CSP，PfSSP2，LSA-1-无重复，MSA-1，SERA，AMA-1和Pfs25的DNA。 此外，有利地，痘病毒是NYVAC痘病毒。 因此，本发明还提供了包含痘病毒的抗疟疾免疫原性或免疫组合物，以及用于在哺乳动物中诱导针对疟疾或疟原虫的免疫原性或免疫应答的方法，包括向哺乳动物施用痘病毒或含有痘病毒的免疫或免疫原性组合物。 哺乳动物可以是一个人。