-
公开(公告)号：US08586339B2
公开(公告)日：2013-11-19
申请号：US13070118
申请日：2011-03-23
CPC分类号： C12N9/18 , C12Y301/01072
摘要： A two-stage heat treatment process is provided to improve the processability of recombinant microbial biomass comprising an enzyme having perhydrolytic activity. More specifically, a process is provided to treat the recombinant microbial biomass comprising a Thermotoga sp. acetyl xylan esterase having perhydrolytic activity such that microfiltration may be used to partially-purify and/or concentrate protein preparations. The acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
摘要翻译： 提供两阶段热处理方法以改善包含具有过水解活性的酶的重组微生物生物质的加工性能。 更具体地，提供了一种处理包含Thermotoga sp。的重组微生物生物质的方法。 具有过水解活性的乙酰木聚糖酯酶，使得微量过滤可用于部分纯化和/或浓缩蛋白质制剂。 乙酰木聚糖酯酶可用于制备适用于各种应用的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。
-
公开(公告)号：US08450091B2
公开(公告)日：2013-05-28
申请号：US13070144
申请日：2011-03-23
CPC分类号： C12N9/18
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号：US08389254B2
公开(公告)日：2013-03-05
申请号：US13070123
申请日：2011-03-23
IPC分类号： C12N9/18
CPC分类号： C12N9/18 , C11D3/38636 , C11D3/3945 , C12P7/40 , D21C5/005 , D21C9/1036
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号：US08329441B2
公开(公告)日：2012-12-11
申请号：US13087800
申请日：2011-04-15
CPC分类号： C11D3/38636 , A01N37/16 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/3947 , C11D3/48 , C12P7/40 , A01N37/02 , A01N37/12 , A01N37/46 , A01N43/16 , A01N59/00 , A01N61/00 , A01N2300/00
摘要： A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
-
公开(公告)号：US08293221B2
公开(公告)日：2012-10-23
申请号：US12572115
申请日：2009-10-01
申请人： Robert Dicosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
发明人： Robert Dicosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
IPC分类号： A61L9/00
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are multi-component formulations for enzymatically producing aqueous solutions of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid formulations comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.
摘要翻译： 本文公开了用于酶促生产适用于例如消毒剂和/或漂白应用的过氧羧酸的水溶液的多组分制剂。 多组分过氧羧酸制剂包含至少一种具有过水解活性的碳水化合物酯酶家族7酶。
-
公开(公告)号：US20120094342A1
公开(公告)日：2012-04-19
申请号：US13330037
申请日：2011-12-19
申请人： Robert Dicosimo , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Richard Alan Reynolds
发明人： Robert Dicosimo , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Richard Alan Reynolds
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are two-component enzymatic peracid generation systems and methods of using such systems wherein the first component comprises a formulation of at least one enzyme catalyst having perhydrolysis activity, a carboxylic acid ester substrate, and a cosolvent and wherein the second component comprises a source of peroxygen in water. The two components are combined to produce an aqueous peracid formulation useful as, e.g., a disinfecting or bleaching agent. Specifically, organic cosolvents are used to control the viscosity of a substrate-containing component and to enhance the solubility of the substrate in an aqueous reaction formulation without causing substantial loss of perhydrolytic activity of the enzyme catalyst.
摘要翻译： 本文公开了双组分酶过酸生成系统和使用这种系统的方法，其中第一组分包含至少一种具有过水解活性的酶催化剂的制剂，羧酸酯底物和共溶剂，并且其中第二组分包含来源 的过氧在水中。 将两种组分组合以产生用作例如消毒剂或漂白剂的含水过酸制剂。 具体地说，使用有机共溶剂来控制含底物的组分的粘度，并提高底物在水性反应制剂中的溶解度，而不会引起酶催化剂的过水解活性的显着损失。
-
公开(公告)号：US20110306667A1
公开(公告)日：2011-12-15
申请号：US13213611
申请日：2011-08-19
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
-
公开(公告)号：US07981643B2
公开(公告)日：2011-07-19
申请号：US13036727
申请日：2011-02-28
申请人： Robert Dicosimo , John Edward Gavagan , Mark Payne
发明人： Robert Dicosimo , John Edward Gavagan , Mark Payne
CPC分类号： C11D3/38636 , A61L2/186 , A61L2/22 , A61L2/23 , A61L2202/17 , A61L2202/24 , C12N9/18 , C12P7/40 , C12Y301/01072
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
-
公开(公告)号：US07923233B1
公开(公告)日：2011-04-12
申请号：US12632454
申请日：2009-12-07
CPC分类号： C12N9/18 , A61L2202/26 , C11D3/38636 , C12P7/40
摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
摘要翻译： 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰海藻酰乙酰木聚糖酯酶基因，以产生特征在于过乙酸形成与过乙酸水解比活性比例（PAAF / PAAH比）增加的酶催化剂， 。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。
-
公开(公告)号：US07867739B2
公开(公告)日：2011-01-11
申请号：US12429576
申请日：2009-04-24
申请人： Robert Dicosimo , Anna Panova , Samuel D. Arthur , Henry Keith
发明人： Robert Dicosimo , Anna Panova , Samuel D. Arthur , Henry Keith
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
-
-
-
-
-
-
-
-
-
搜索结果
91 条记录 (耗时 0.066 秒)
