公开(公告)号：US09422384B2

公开(公告)日：2016-08-23

申请号：US14745815

申请日：2015-06-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US09227199B2

公开(公告)日：2016-01-05

申请号：US12601772

申请日：2008-05-29

Applicant: Darren Ellis ,  Tine Borgen Hildisch ,  Jeremy Mundy ,  Erlend Ragnhildstveit ,  Richard Johan Toften ,  Kornelija Zgonc ,  Malin Karlsson ,  Gaute Brede ,  Lars Noekleby

Inventor： Darren Ellis ,  Tine Borgen Hildisch ,  Jeremy Mundy ,  Erlend Ragnhildstveit ,  Richard Johan Toften ,  Kornelija Zgonc ,  Malin Karlsson ,  Gaute Brede ,  Lars Noekleby

IPC: B03C1/28 ,  B01L9/06

CPC classification number: B03C1/288 ,  B01L9/06 ,  B03C1/0332 ,  B03C2201/18 ,  B03C2201/26

Abstract: The present disclosure relates to a magnetizing portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetizing portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetizing portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.

Abstract translation: 本公开涉及一种用于在磁选机中提供高梯度磁场的磁化部分。 磁化部分包括至少一个磁性组件。 所述至少一个磁性组件包括：多个磁体，其中每个磁体具有北极，南极和在所述北极和南极之间延伸的磁体轴线，并且所述多个磁体在至少一个方向上彼此上下排列 基本上垂直于每个磁体的轴线，使得相邻磁体的北极和南极交替布置并且在相邻磁体之间提供空间; 以及布置在相邻磁体之间的空间中的至少一个非磁性间隔装置。 本公开还涉及包括所述磁化部分中的至少一个的磁分离装置以及使用磁分离装置分离磁性标记颗粒的方法。

公开(公告)号：US09139665B2

公开(公告)日：2015-09-22

申请号：US14082336

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete I. Modahl ,  Nini H. Kjus ,  Astrid E. Molteberg ,  Diem Tran ,  Jo Aaserud ,  Talha M. Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F8/32 ,  C08F8/34 ,  C08F6/24 ,  C07F7/18 ,  C08F130/08 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C12Q1/68

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09119807B2

公开(公告)日：2015-09-01

申请号：US13689530

申请日：2012-11-29

Applicant: Life Technologies AS

Inventor： Tanja Aarvak ,  Anne-Marie Rasmussen ,  Gunnar Kvalheim ,  Walter Gabriell Borelli Piedras ,  Anne Brunsvig

IPC: C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  A61K31/12 ,  C12N5/0783

CPC classification number: C12N5/0637 ,  A61K31/12 ,  A61K35/17 ,  A61K2035/122 ,  C12N5/0636 ,  C12N2501/04 ,  C12N2501/2302 ,  C12N2501/2304 ,  C12N2501/998 ,  C12N2501/999

Abstract: Methods are disclosed for the generation of immunosuppressive regulatory T cells. The methods can include contacting a population of CD4+CD25− T cells with a T cell receptor (TCR)/CD3 activator, a TCR co-stimulator activator, and rapamycin. Kits for the generation of immunosuppressive regulatory T cells, methods of use, and cell populations are also disclosed.

Abstract translation: 公开了用于产生免疫抑制性调节性T细胞的方法。 所述方法可以包括使CD4 + CD25-T细胞群与T细胞受体（TCR）/ CD3激活剂，TCR共刺激因子激活剂和雷帕霉素接触。 还公开了用于产生免疫抑制性调节性T细胞，使用方法和细胞群的试剂盒。

公开(公告)号：US09067954B2

公开(公告)日：2015-06-30

申请号：US14081263

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini H. Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08L9/00 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US09062080B2

公开(公告)日：2015-06-23

申请号：US14081224

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini H. Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F236/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US20140142254A1

公开(公告)日：2014-05-22

申请号：US14082368

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Geir FONNUM ,  Grete I. MODAHL ,  Nini H. KJUS ,  Astrid E. MOLTEBERG ,  Diem TRAN ,  Jo AASERUD ,  Talha M. GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F8/34 ,  C08F8/32

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US20140073715A1

公开(公告)日：2014-03-13

申请号：US14082419

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Geir FONNUM ,  Grete I. MODAHL ,  Nini H. KJUS ,  Astrid E. MOLTEBERG ,  Diem TRAN ,  Jo AASERUD ,  Talha M. GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F222/38

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US08574515B2

公开(公告)日：2013-11-05

申请号：US12601783

申请日：2008-05-29

Applicant: Darren Ellis ,  Tine Borgen Hildisch ,  Jeremy Mundy ,  Erlend Ragnhildstveit ,  Richard Johan Toften ,  Kornelija Zgonc ,  Gaute Brede ,  Lars Noekleby

Inventor： Darren Ellis ,  Tine Borgen Hildisch ,  Jeremy Mundy ,  Erlend Ragnhildstveit ,  Richard Johan Toften ,  Kornelija Zgonc ,  Gaute Brede ,  Lars Noekleby

IPC: B01D33/06

CPC classification number: B03C1/288 ,  B01L9/06 ,  B03C1/0332 ,  B03C2201/18 ,  B03C2201/26

Abstract: The disclosure relates to a magnetic separating device for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion (1) having a magnetising portion (3) for providing a magnetic field and a surface by means of which the body portion may stand on a supporting surface; and a sample vessel retaining portion (4) for retaining at least one sample vessel (5), wherein, the magnetising portion (3) is configured to conform at least approximately to at least a substantial portion of the longitudinal profile of at least one sample vessel (5); the sample vessel retaining portion (4) is configured to retain at least one sample vessel (5) such that at least a portion of the contents of the sample vessel (5) is visible to a user; and the sample vessel retaining portion (4) is configured to be mountable on the magnetising portion so that in use, the at least one sample vessel is subject to the magnetic field of the magnetising portion (3). The disclosure further relates to a kit of parts and a method for isolating magnetically labelled particles from a non-magnetic medium using the magnetic separation device.

Abstract translation: 本公开涉及一种用于从非磁性介质中分离磁性标记的颗粒的磁分离装置，其包括具有用于提供磁场的磁化部分（3）的主体部分（1）和主体部分可以支撑的表面 在支撑面上 以及用于保持至少一个样品容器（5）的样品容器保持部分（4），其中，所述磁化部分（3）构造成使至少大致至少大致至少一部分至少一个样品的纵向轮廓 船只（5）; 样品容器保持部分（4）构造成保持至少一个样品容器（5），使得样品容器（5）的内容物的至少一部分对使用者是可见的; 并且样品容器保持部（4）被构造成可安装在磁化部分上，使得在使用中，至少一个样品容器受到磁化部分（3）的磁场的影响。 本发明还涉及使用磁选机从非磁性介质中分离磁性标记的颗粒的一组零件和方法。

公开(公告)号：US20130164312A1

公开(公告)日：2013-06-27

申请号：US13689530

申请日：2012-11-29

Applicant: Life Technologies AS

Inventor： Tanja AARVAK ,  Anne-Marie RASMUSSEN ,  Gunnar KVALHEIM ,  Walter Gabriell Borelli PIEDRAS ,  Anne BRUNSVIG

IPC: C12N5/0783

CPC classification number: C12N5/0637 ,  A61K31/12 ,  A61K35/17 ,  A61K2035/122 ,  C12N5/0636 ,  C12N2501/04 ,  C12N2501/2302 ,  C12N2501/2304 ,  C12N2501/998 ,  C12N2501/999

Abstract: Methods are disclosed for the generation of immunosuppressive regulatory T cells. The methods can include contacting a population of CD4+CD25− T cells with a T cell receptor (TCR)/CD3 activator, a TCR co-stimulator activator, and rapamycin. Kits for the generation of immunosuppressive regulatory T cells, methods of use, and cell populations are also disclosed.

Abstract translation: 公开了用于产生免疫抑制性调节性T细胞的方法。 所述方法可以包括使CD4 + CD25-T细胞群与T细胞受体（TCR）/ CD3激活剂，TCR共刺激因子激活剂和雷帕霉素接触。 还公开了用于产生免疫抑制性调节性T细胞，使用方法和细胞群的试剂盒。