公开(公告)号：US20140050766A1

公开(公告)日：2014-02-20

申请号：US13985388

申请日：2012-02-13

Applicant: Shulamit Levenberg ,  Ayelet Lesman

Inventor： Shulamit Levenberg ,  Ayelet Lesman

IPC: A61K35/12 ,  A61K9/14

CPC classification number: A61K35/33 ,  A61K9/148 ,  A61L27/225 ,  A61L27/3808 ,  A61L27/3886 ,  A61L27/48 ,  A61L27/507

Abstract: A three-dimensional fibrin engineered tissue construct is provided selected from: (i) a fibrin gel matrix comprising a combination of tissue-specific cells and at least one type of vascular cells; and (ii) a hybrid scaffold of fibrin gel and a polymeric synthetic scaffold comprising at least one type of vascular cells or a combination of tissue-specific cells and at least one type of vascular cells.

Abstract translation: 提供三维纤维蛋白工程化组织构建体，其选自：（i）包含组织特异性细胞和至少一种类型的血管细胞的组合的纤维蛋白凝胶基质; 和（ii）纤维蛋白凝胶的混合支架和包含至少一种类型的血管细胞或组织特异性细胞和至少一种类型的血管细胞的组合的聚合合成支架。

公开(公告)号：US20140012407A1

公开(公告)日：2014-01-09

申请号：US13968313

申请日：2013-08-15

Applicant: Organovo, Inc.

Inventor： Keith Murphy ,  Scott Dorfman ,  Nathan Smith ,  Larry Bauwens ,  Ian Sohn ,  Tim McDonald ,  Chris Leigh-Lancaster ,  Richard Jin Law

IPC: B28B1/00

CPC classification number: A61L27/3808 ,  A61L27/3826 ,  B01L3/0268 ,  B28B1/001 ,  B29C64/106 ,  B29C64/112 ,  B29C64/118 ,  B29C64/20 ,  B33Y30/00 ,  B33Y50/02 ,  B33Y70/00 ,  B41J3/407 ,  C12M21/08 ,  C12M33/00

Abstract: Described herein are bioprinters comprising: one or more printer heads, wherein a printer head comprises a means for receiving and holding at least one cartridge, and wherein said cartridge comprises contents selected from one or more of: bio-ink and support material; a means for calibrating the position of at least one cartridge; and a means for dispensing the contents of at least one cartridge. Further described herein are methods for fabricating a tissue construct, comprising: a computer module receiving input of a visual representation of a desired tissue construct; a computer module generating a series of commands, wherein the commands are based on the visual representation and are readable by a bioprinter; a computer module providing the series of commands to a bioprinter; and the bioprinter depositing bio-ink and support material according to the commands to form a construct with a defined geometry.

Abstract translation: 本文描述的生物打印机包括：一个或多个打印头，其中打印头包括用于接收和保持至少一个盒的装置，并且其中所述盒包括从以下中的一个或多个选择的内容：生物油墨和支撑材料; 用于校准至少一个盒的位置的装置; 以及用于分配至少一个盒的内容物的装置。 本文进一步描述的是用于制造组织结构的方法，包括：计算机模块，其接收所需组织结构的视觉表示的输入; 生成一系列命令的计算机模块，其中所述命令基于所述视觉表示，并且可由生物打印机读取; 为生物打印机提供一系列命令的计算机模块; 并且生物印刷机根据命令沉积生物油墨和支撑材料以形成具有确定的几何形状的构造。

公开(公告)号：US20130344114A1

公开(公告)日：2013-12-26

申请号：US13607397

申请日：2012-09-07

Applicant: Nai-Jen Chang ,  Ming-Long Yeh ,  Chih-Chan Lin

Inventor： Nai-Jen Chang ,  Ming-Long Yeh ,  Chih-Chan Lin

IPC: A61K35/12 ,  A61K9/00 ,  C12N5/071 ,  A61P19/04

CPC classification number: A61L27/20 ,  A61K35/00 ,  A61L27/18 ,  A61L27/3808 ,  A61L27/56 ,  A61L2430/06 ,  C12N5/0692 ,  C12N2533/40 ,  C08L67/04 ,  C08L67/02 ,  C08L71/02 ,  C08L89/06 ,  C08L5/08

Abstract: A composition for repairing cartilage tissues includes a scaffold and a plurality of endothelial progenitor cells. The endothelial progenitor cells adhere on the scaffold. A method of making the composition for repairing cartilage tissue is also disclosed. This is advantageous for safely and quickly repairing cartilage tissues by using the composition and the manufacturing method thereof.

Abstract translation: 用于修复软骨组织的组合物包括支架和多个内皮祖细胞。 内皮祖细胞粘附在支架上。 还公开了制备用于修复软骨组织的组合物的方法。 这对于通过使用组合物及其制造方法来安全和快速地修复软骨组织是有利的。

公开(公告)号：US20130039966A1

公开(公告)日：2013-02-14

申请号：US13653028

申请日：2012-10-16

Applicant: SEARETE LLC

Inventor： Ed Harlow ,  Edward K.Y. Jung ,  Robert Langer ,  Eric C. Leuthardt ,  Lowell L. Wood, JR.

IPC: A61K35/32 ,  A61P3/00

CPC classification number: A61K47/46 ,  A61K9/0024 ,  A61K47/48776 ,  A61K47/6901 ,  A61K2035/126 ,  A61L27/3608 ,  A61L27/3808 ,  B33Y80/00 ,  C12N5/0654 ,  C12N2510/00

Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.

公开(公告)号：US08367384B2

公开(公告)日：2013-02-05

申请号：US11974852

申请日：2007-10-15

Applicant: Ed Harlow ,  Edward K. Y. Jung ,  Robert Langer ,  Eric C. Leuthardt ,  Lowell L. Wood, Jr.

Inventor： Ed Harlow ,  Edward K. Y. Jung ,  Robert Langer ,  Eric C. Leuthardt ,  Lowell L. Wood, Jr.

IPC: C12N9/00 ,  A61K9/48 ,  A61K39/40

CPC classification number: A61L27/54 ,  A61F2250/0023 ,  A61K45/06 ,  A61K47/6901 ,  A61K2035/126 ,  A61L27/3608 ,  A61L27/38 ,  A61L27/3808 ,  A61L2430/38 ,  B33Y80/00 ,  C12N5/0654 ,  C12N2510/00

Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.

公开(公告)号：US20130018454A1

公开(公告)日：2013-01-17

申请号：US13511511

申请日：2010-11-24

Applicant: Peter I. Lelkes ,  Mengyan Li ,  Anat Perets ,  Pimporn Uttayarat ,  Robert J. Levy ,  Russell J. Composto

Inventor： Peter I. Lelkes ,  Mengyan Li ,  Anat Perets ,  Pimporn Uttayarat ,  Robert J. Levy ,  Russell J. Composto

IPC: A61F2/04 ,  A61F2/06 ,  B05D5/00

CPC classification number: A61F2/0077 ,  A61F2/06 ,  A61F2/062 ,  A61F2002/0081 ,  A61F2002/0086 ,  A61F2240/001 ,  A61F2310/00005 ,  A61F2310/00389 ,  A61L27/18 ,  A61L27/3808 ,  A61L27/3813 ,  A61L27/3817 ,  A61L27/3821 ,  A61L27/507 ,  A61L2430/32 ,  D01D5/0076 ,  D01F6/70 ,  D10B2331/041 ,  D10B2509/06 ,  C08L67/04

Abstract: The present invention relates to a hybrid graft and methods of generating the hybrid graft. The hybrid graft comprises an exterior surface and a luminal surface. The luminal surface comprises a micropattern of grooves to which cells adhere and orient along. The exterior surface comprises electrospun microfibers wherein the microfibers provide mechanical properties to the graft. The hybrid graft is capable supporting endothelial cell attachment, endothelial cell alignment, cell proliferation, and maintaining their in vivo function. The graft of the invention can recapitulate the in vivo morphology and function of natural vascular endothelium.

公开(公告)号：US20120244617A1

公开(公告)日：2012-09-27

申请号：US13427843

申请日：2012-03-22

Applicant: Seyedhamed Alavi ,  Arash Kheradvar

Inventor： Seyedhamed Alavi ,  Arash Kheradvar

IPC: C12N5/0775 ,  C12N5/071

CPC classification number: A61F2/2418 ,  A61F2/07 ,  A61F2/2415 ,  A61F2/90 ,  A61F2240/001 ,  A61K35/12 ,  A61L27/047 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3826 ,  A61L27/3886 ,  A61L27/446 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/414 ,  A61L2400/18 ,  A61L2430/20 ,  C12N5/0068 ,  C12N5/0691 ,  C12N2501/15 ,  C12N2533/10 ,  C12N2533/54 ,  C08L89/06

Abstract: Described is a scaffold that is strong enough to resist forces that exist inside a body, while possessing biocompatible surfaces. The scaffold is formed of a layer of mesh (e.g., Stainless Steel or Nitinol) that is tightly enclosed by a multi-layer biological matrix. The biological matrix can include three layers, such a first layer (smooth muscle cells) formed directly on the metal mesh, a second layer (fibroblast/myofibroblast cells) formed on the first layer, and a third layer (endothelial cells) formed on the second layer. The scaffold can be formed to operate as a variety of tissues, such as a heart valve or a vascular graft. For example, the mesh and corresponding biological matrix can be formed as leaflets, such that the scaffold is operable as a tissue heart valve.

公开(公告)号：US08192348B2

公开(公告)日：2012-06-05

申请号：US10562955

申请日：2004-07-01

Applicant: Robert T. Tranquillo ,  Jeffrey Ross ,  Morayma Reyes

Inventor： Robert T. Tranquillo ,  Jeffrey Ross ,  Morayma Reyes

IPC: A61F2/04

CPC classification number: A61L27/507 ,  A61L27/3808 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3843 ,  A61L27/3891 ,  C12N5/0691 ,  C12N2500/25 ,  C12N2501/11 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/39 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/11 ,  C12N2506/1353 ,  C12N2533/30 ,  C12N2533/54 ,  C12N2533/56

Abstract: The present invention relates to engineered blood vessels and methods of making such vessels using matrices comprising endothelial and smooth muscle cells, or cells capable of differentiating into endothelial and smooth muscle cell lineages (e.g., stem cells, or the progenitors thereof).

Abstract translation: 本发明涉及工程化血管和使用包含内皮和平滑肌细胞或能够分化成内皮和平滑肌细胞谱系的细胞（例如，干细胞或其祖细胞）的基质制备这种血管的方法。

公开(公告)号：US20110270412A1

公开(公告)日：2011-11-03

申请号：US13054450

申请日：2009-07-16

Applicant: Leon M. Bellan ,  Harold Craighead ,  Jason A. Spector

Inventor： Leon M. Bellan ,  Harold Craighead ,  Jason A. Spector

IPC: A61F2/02 ,  B29C69/02 ,  B29C47/06 ,  A61M31/00

CPC classification number: A61L27/3808 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/412 ,  A61L2300/414 ,  A61L2300/426 ,  A61L2300/602

Abstract: The first aspect of the present invention is directed to a method of producing a vascular network preform (VNP). This method involves forming a network of elongate fibers and at least one elongate structure from a sacrificial material. The diameter of the elongate structure is greater than that of the elongate fibers. The network of elongate fibers is placed in contact with at least one elongate structure either following or during forming the network of elongate fibers or forming the at least one elongate structure. A matrix is applied around the network of elongate fibers, in contact with the at least one elongate structure. The network of elongate fibers and elongate structure, within the matrix is sacrificed to form a preform. The resulting preform contains a vascular network of fine diameter tubes in contact with at least one elongate passage having a diameter greater than that of the fine diameter tubes. The resulting solid preform and methods of using it are also disclosed.

Abstract translation: 本发明的第一方面涉及一种生产血管网预制件（VNP）的方法。 该方法包括从牺牲材料形成细长纤维网络和至少一个细长结构。 细长结构的直径大于细长纤维的直径。 细长纤维的网络放置成在形成细长纤维的网络或形成至少一个细长结构之间或之后的至少一个细长结构接触。 矩阵围绕细长纤维的网络施加，与至少一个细长结构接触。 处于基体内的细长纤维和细长结构的网络以形成预制件。 所得到的预制件包含与直径大于细直径管的直径的至少一个细长通道接触的细直径管的血管网络。 还公开了所得到的固体预制件及其使用方法。

公开(公告)号：US20100310630A1

公开(公告)日：2010-12-09

申请号：US12597641

申请日：2003-04-27

Applicant: Stephan Reichel

Inventor： Stephan Reichel

IPC: A61K9/00 ,  B05D3/02 ,  C12N5/071 ,  A61K35/12 ,  A61P17/02 ,  A61P27/02

CPC classification number: C12N5/0068 ,  A61L15/32 ,  A61L27/34 ,  A61L27/3808 ,  A61L27/3813 ,  C12N2533/50 ,  C08L89/04

Abstract: The invention makes available a keratin coating of a support substrate and a method for the production thereof. This keratin coating is suitable particularly for the in vitro or in vivo culturing of epithelial or endothelial cells and, because of its optical transparency, for microscopy. The keratin coating is prepared by applying or coating keratin in the form of nanoparticles from an aqueous keratin solution or keratin suspension. The solution or suspension does not contain any reducing compounds. The keratin solution is prepared preferably from hair, such as human hair. For the preparation of the coating, keratin is brought into solution or into a suspension of nanoparticles by mixing the keratin with an aqueous composition which contains thiourea, urea, and mercaptoethanol.

Abstract translation: 本发明提供了支撑基材的角蛋白涂层及其生产方法。 这种角蛋白涂层特别适用于上皮细胞或内皮细胞的体外或体内培养，并且由于其光学透明度而适用于显微镜。 通过从角质水溶液或角蛋白悬浮液中施加或涂覆纳米颗粒形式的角蛋白来制备角蛋白涂层。 溶液或悬浮液不含任何还原性化合物。 角蛋白溶液优选由头发制成，例如人发。 为了制备涂层，通过将角蛋白与含有硫脲，尿素和巯基乙醇的水性组合物混合，将角蛋白引入溶液或纳米颗粒的悬浮液中。