公开(公告)号：US20180296640A1

公开(公告)日：2018-10-18

申请号：US15769132

申请日：2016-11-07

申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： Terry Vanden Hoek ,  Xiangdong Zhu ,  Jing Li

IPC分类号： A61K38/17 ,  A61P9/04

CPC分类号： A61K38/1761 ,  A61K31/455 ,  A61K2300/00 ,  A61P9/04 ,  C07K16/00 ,  C07K2319/00 ,  C12N5/10 ,  C12N9/1205 ,  C12N9/16 ,  C12Y301/03016 ,  C40B40/08 ,  G01N33/6893 ,  G01N2800/325 ,  G01N2800/52

摘要： Modified peptides based on C-terminal PDZ binding domains of PTEN and PHLPP, or PDK1 interacting fragment of PKN2 are described as are methods of using the modified peptides for blocking the activity of PTEN, PHLPP and PKN2 and treating sudden cardiac arrest. A method for guiding treatment of cardiac arrest based on sorbitol or taurine levels is also provided.

公开(公告)号：US20180273982A1

公开(公告)日：2018-09-27

申请号：US15873761

申请日：2018-01-17

申请人： Danisco US Inc. ,  The Goodyear Tire & Rubber Company

发明人： Marguerite A. CERVIN ,  Gopal K. CHOTANI ,  Frank J. FEHER ,  Richard LA DUCA ,  Joseph C. MCAULIFFE ,  Andrei MIASNIKOV ,  Caroline M. PERES ,  Aaron S. PUHALA ,  Karl J. SANFORD ,  Fernando VALLE ,  Gregory M. WHITED

IPC分类号： C12P5/00 ,  C12N9/12 ,  C12N9/88 ,  C12N9/10 ,  C12N15/52 ,  C12N9/90

CPC分类号： C12P5/007 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y203/01009 ,  C12Y207/01036 ,  C12Y401/01033 ,  C12Y402/03027 ,  C12Y503/03002 ,  Y02E50/343

摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

公开(公告)号：US20180258453A1

公开(公告)日：2018-09-13

申请号：US15912636

申请日：2018-03-06

申请人： DePuy Synthes Products, Inc.

发明人： Douglas D. Buechter

IPC分类号： C12P19/04 ,  C12N9/12 ,  C12N1/20 ,  A61L27/20

CPC分类号： C12P19/04 ,  A61L27/20 ,  C07K14/245 ,  C12N1/20 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/90 ,  C12R1/02 ,  C12Y207/01059

摘要： The present disclosure is directed to microorganisms that are genetically modified to express at least one exogenous enzyme involved in N-acetylglucosamine uptake and metabolism. Methods for the production of an exopolysaccharide are also disclosed. The exopolysaccharide comprises N-acetylglucosamine and D-glucose and may be used as a bioresorbable implant for soft tissue repair, replacement, or augmentation.

公开(公告)号：US10041094B2

公开(公告)日：2018-08-07

申请号：US14233830

申请日：2011-07-22

申请人： R. Rogers Yocum ,  Theron Hermann ,  Xiaohui Hu

发明人： R. Rogers Yocum ,  Theron Hermann ,  Xiaohui Hu

IPC分类号： C12P7/46 ,  C12N1/20 ,  C12N15/52 ,  C07K14/245 ,  C12N9/04 ,  C12N9/12 ,  C12P7/56

CPC分类号： C12P7/46 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/52 ,  C12P7/56 ,  C12Y101/01008 ,  C12Y101/05003

摘要： The present invention is in the field of producing organic acids and other useful chemicals via biological fermentation using glycerol as a source of carbon. Novel microorganisms and fermentation processes are described that are capable of converting glycerol to useful organic acids in high yield and high purity.

公开(公告)号：US20180195051A1

公开(公告)日：2018-07-12

申请号：US15849122

申请日：2017-12-20

申请人： Creatus Biosciences Inc.

发明人： Zongli Luo ,  Hendrik Jurgens Jansen van Vuuren ,  Allan George DeBono ,  Andrew Taplin Ferguson

IPC分类号： C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12P7/06 ,  C12N9/12

CPC分类号： C12N9/0006 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/16 ,  C12P7/06 ,  C12Y101/01175 ,  C12Y101/01307 ,  C12Y202/01002 ,  C12Y207/01017 ,  C12Y301/03041 ,  Y02E50/17

摘要： Provided herein are non-naturally occurring microbial organisms having increased xylose metabolism and increased production of ethanol using xylose as a substrate, as well as methods to make and use these microbial organisms to produce ethanol.

公开(公告)号：US10006047B2

公开(公告)日：2018-06-26

申请号：US15019838

申请日：2016-02-09

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Mark A. Kay ,  Jiamiao Lu

IPC分类号： C12N15/85 ,  A61K31/713 ,  C07K14/245 ,  C12N9/12 ,  C12N9/86

CPC分类号： C12N15/85 ,  A61K31/713 ,  C07K14/245 ,  C12N9/1205 ,  C12N9/86

摘要： Provided are nucleic acids and expression vectors having a non-silencing selectable marker gene, and methods of using the same. A subject expression vector includes an expression cassette and a non-silencing selectable marker gene. In some cases, the non-silencing selectable marker gene provides for drug resistance for prokaryotic cells, and includes a nucleotide sequence that (i) encodes a drug selectable marker protein; (ii) is operably linked to a promoter functional in prokaryotic cells, and (iii) includes an increased A/T content relative to a corresponding wild type nucleotide sequence. In some cases, the non-silencing selectable marker gene provides for drug resistance for prokaryotic cells, and includes a nucleotide sequence that (i) encodes a drug selectable marker protein; (ii) is operably linked to a promoter functional in prokaryotic cells, and (iii) has an A/T content in a range of from 52% to 70%.

公开(公告)号：US09988688B2

公开(公告)日：2018-06-05

申请号：US14870154

申请日：2015-09-30

申请人： Cell Signaling Technology, Inc.

发明人： Klarisa Rikova ,  Herbert Haack ,  Laura Sullivan ,  Ailan Guo ,  Anthony Possemato ,  Joan MacNeill ,  Ting-Lei Gu ,  Jian Yu

IPC分类号： A61K38/00 ,  C12N9/12 ,  G01N33/574 ,  G01N33/68 ,  A61K31/713 ,  C07K14/47 ,  C12Q1/68 ,  C12Q1/6886

CPC分类号： C12Q1/6886 ,  A61K31/713 ,  A61K38/00 ,  C07K14/47 ,  C07K2319/00 ,  C12N9/12 ,  C12N9/1205 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y207/10001 ,  G01N33/57423 ,  G01N33/57484 ,  G01N33/6893 ,  G01N2333/912 ,  G01N2333/9121

摘要： Novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have been identified herein in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides.

公开(公告)号：US20180148732A1

公开(公告)日：2018-05-31

申请号：US15823658

申请日：2017-11-28

申请人： The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organizat

发明人： David GRANOT ,  Ofer Stein

IPC分类号： C12N15/82 ,  C12N9/12

CPC分类号： C12N15/8247 ,  C12N9/1205 ,  C12N15/8234 ,  C12N15/8267 ,  C12Y207/01004

摘要： A method of modifying oil content of a plant is provided. The method comprising: (a) modulating activity or expression of a fructokinase (FRK) in seeds of a plurality of plants; and (b) selecting a seeds of the plurality of plants or progeny thereof exhibiting the modified oil content as compared to the oil content of seeds of the same species not subjected to step (a).

公开(公告)号：US20180087059A1

公开(公告)日：2018-03-29

申请号：US15833890

申请日：2017-12-06

申请人： CALYSTA, INC.

发明人： Joshua A. SILVERMAN ,  Effendi LEONARD ,  Renee M. SAVILLE

IPC分类号： C12N15/52 ,  C12N1/20 ,  C12N9/12 ,  C12N9/04 ,  C12N9/00

CPC分类号： C12N15/52 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/93 ,  C12Y101/01008 ,  C12Y101/01027 ,  C12Y207/0103 ,  C12Y602/01001

摘要： The present disclosure relates to genetically engineered methanotrophic bacteria with the capability of growing on a multi-carbon substrate (e.g., glycerol) as a primary or sole carbon source and methods for growing methanotrophic bacteria on the multi-carbon substrate.

公开(公告)号：US09926606B2

公开(公告)日：2018-03-27

申请号：US14960681

申请日：2015-12-07

申请人： The Johns Hopkins University

发明人： Alberto Bardelli ,  D. Williams Parsons ,  Victor Velculescu ,  Kenneth W. Kinzler ,  Bert Vogelstein

IPC分类号： C12Q1/00 ,  C12Q1/68 ,  C12N9/12 ,  C12Q1/48 ,  G01N33/574 ,  C12Q1/02 ,  A61K31/506

CPC分类号： C12Q1/6886 ,  A61K31/506 ,  C12N9/1205 ,  C12Q1/025 ,  C12Q1/485 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q2600/136 ,  C12Q2600/156 ,  G01N33/574

摘要： Protein kinases are important signaling molecules involved in tumorigenesis. Mutational analysis of the human tyrosine kinase gene family (98 genes) identified somatic alterations in ˜20% of colorectal cancers, with the majority of mutations occurring in NTRK3, FES, GUCY2F and a previously uncharacterized tyrosine kinase gene called MCCK/MLK4. Most alterations were in conserved residues affecting key regions of the kinase domain. These data represent a paradigm for the unbiased analysis of signal transducing genes in cancer and provide useful targets for therapeutic intervention.