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    PROBES AND A METHYLATION IN SITU HYBRIDIZATION ASSAY
    41.
    发明申请
    PROBES AND A METHYLATION IN SITU HYBRIDIZATION ASSAY 审中-公开

    公开(公告)号：US20180187246A1

    公开(公告)日：2018-07-05

    申请号：US15740255

    申请日：2016-07-12

    Applicant: UNIVERSITEIT GENT

    Inventor： Marusya Lieveld ,  Wim Van Criekinge ,  Davy Vanden Broeck

    IPC: C12Q1/6827

    CPC classification number: C12Q1/6827 ,  C12Q1/682 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q2523/125 ,  C12Q2525/161 ,  C12Q2537/163 ,  C12Q2565/102 ,  C12Q2600/154

    Abstract: The disclosure relates to the field of molecular pathology (for example, cancer diagnosis, prognosis, treatment and/or therapy prediction) through the detection of RNA, mutations, copy number changes and determination of the methylation status of specific sequences of the genome of individual patients in hybridization assays (southern blot, ISH, dot blot) including in situ determination of the methylation status of specific sequences of the genome of individual patients in individual cells. More specifically, this disclosure relates to: a) target-specific probes covalently attached to a labeled tail, b) the synthesis method of said the probe, c) the usage of said the probe such as an in situ hybridization-based method to correlate the methylation status of a promoter region of a gene in a biopsy or cytology specimen of a patient to the morphology and localization in that specimen, and d) kits comprising the target-specific probes. The latter method and products allow detection of (epi) genetic changes in specific cell types of histological or cytological (cancer) specimens or on membranes that will contribute to scientific research and that will help physicians to accurately diagnose diseases and/or start an appropriate treatment.

    Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
    45.
    发明授权
    Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression 有权

    公开(公告)号：US09909169B2

    公开(公告)日：2018-03-06

    申请号：US14574181

    申请日：2014-12-17

    Applicant: Roche Molecular Systems, Inc.

    Inventor： Stephen G. Will

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q1/6806 ,  C12Q2525/117 ,  C12Q2527/107 ,  C12Q2537/159 ,  C12Q2537/163

    Abstract: A method is provided for allele-specific amplification, utilizing a blocking oligonucleotide including at least one nucleotide with a base covalently modified at the exocyclic amino group, the blocking oligonucleotide being perfectly complementary to a wild type (WT) sequence when hybridized forming a first complex having a first melting temperature (Tm), the blocking oligonucleotide being partially non-complementary, at one or more nucleotides, to a target mutant (MT) sequence when hybridized forming a second complex having a second melting temperature (Tm), wherein the first Tm is higher than the second Tm and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the blocking oligonucleotide becomes unhybridized from the target MT sequence during amplification but remains hybridized with the WT sequence inhibiting amplification of the WT sequence utilizing a polymerase lacking 5′-3′ nuclease activity.

    Methods for detecting nucleic acid sequence variants
    46.
    发明授权
    Methods for detecting nucleic acid sequence variants 有权

    公开(公告)号：US09834817B2

    公开(公告)日：2017-12-05

    申请号：US13841842

    申请日：2013-03-15

    Applicant: Biocept, Inc. ,  Aegea Biotechnologies

    Inventor： Lyle Arnold

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6858 ,  C12Q2600/112 ,  C12Q2537/163

    Abstract: The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.

    METHOD TO INCREASE SENSITIVITY OF NEXT GENERATION SEQUENCING
    49.
    发明申请
    METHOD TO INCREASE SENSITIVITY OF NEXT GENERATION SEQUENCING 审中-公开
    提高下一代测序灵敏度的方法

    公开(公告)号：US20160340725A1

    公开(公告)日：2016-11-24

    申请号：US15134302

    申请日：2016-04-20

    Applicant: NEOGENOMICS LABORATORIES, INC.

    Inventor： Maher Albitar

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2537/163

    Abstract: A method for detecting a low-occurrence mutation in isolated DNA adds a blocking probe to reagents during amplification of the isolated DNA. The blocking probe is an oligonucleotide complementary to wild-type DNA corresponding to the sample. The blocking probe spans a site of a suspected mutation within a region of interest in the isolated DNA. After amplification, fragments of the amplified DNA is sequenced using next generating sequencing and an output is generated to display the sequenced fragments. In some embodiments, the blocking probe is locked nucleic acid (LNA).

    Abstract translation: 用于检测分离的DNA中的低发生突变的方法在扩增分离的DNA期间向试剂添加封闭探针。 阻断探针是与对应于样品的野生型DNA互补的寡核苷酸。 阻断探针跨越分离的DNA中感兴趣区域内的疑似突变的位点。 扩增后，使用下一个生成测序对扩增的DNA的片段进行测序，并产生输出以显示测序的片段。 在一些实施方案中，阻断探针是锁定核酸（LNA）。

    METHOD FOR ANALYZING MULTIPLE NUCLEIC ACID TARGETS
    50.
    发明申请
    METHOD FOR ANALYZING MULTIPLE NUCLEIC ACID TARGETS 有权
    分析多种核酸目标的方法

    公开(公告)号：US20160265030A1

    公开(公告)日：2016-09-15

    申请号：US15049053

    申请日：2016-02-20

    Applicant: Panasonic Intellectual Property Management Co., Ltd.

    Inventor： MASAHIKO TSUKUDA

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q2527/107 ,  C12Q2537/163 ,  C12Q2563/159

    Abstract: A detection device includes a PCR processor for conducting a PCR process on a first drop to a fourth drop flowing in a flow channel, a boundary detector for detecting intensities of fluorescence outputted from the first drop to the fourth drop after the PCR process and acquiring boundaries between the first drop to the fourth drop flowing in a flow channel based on the intensities of fluorescence, and a detector for acquiring a number of the second drop and the fourth drop having an intensity of fluorescence greater than or equal to a first threshold based on the intensity of fluorescence and boundaries between the first drop to the fourth drop, and detecting whether or not the objective nucleic acid target includes at least one selected from the group consisting of a first nucleic acid target and a second nucleic acid target based on the number of the second drop and the fourth drop.

    Abstract translation: 检测装置包括：PCR处理器，用于在流道中流动的第一滴到第四滴处进行PCR过程;边界检测器，用于在PCR过程之后检测从第一滴到第四滴输出的荧光的强度，并获取边界 在基于荧光强度的流路中流动的第一液滴与第四液滴之间，以及用于获取多个第二液滴的检测器和具有大于或等于第一阈值的荧光强度的检测器，基于 荧光强度和第一滴至第四滴之间的边界，并且基于该数目检测目标核酸靶是否包括选自由第一核酸靶和第二核酸靶组成的组中的至少一个 的第二滴和第四滴。

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