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156 条记录 (耗时 0.032 秒)

    Compositions and methods for modulating Syk function
    41.
    发明申请
    Compositions and methods for modulating Syk function 审中-公开
    调节Syk功能的组成和方法

    公开(公告)号：US20030113828A1

    公开(公告)日：2003-06-19

    申请号：US10293862

    申请日：2002-11-12

    发明人： Mark H. Ginsberg ,  Darren G. Woodside

    IPC分类号： A61K038/16 ,  C12Q001/48 ,  C12N009/12

    CPC分类号： C12Q1/485 ,  G01N2333/70557 ,  G01N2500/04

    摘要： Methods are provided for identifying agents which modulate the interaction of tyrosine kinases of the Syk family with integrins. Also provided are compositions and methods for using these agents to modulate tyrosine kinases of the Syk family and to treat disease such as thrombosis, inflammation, metastasis and tumor cell growth in a subject.

    摘要翻译： 提供了用于鉴定调节Syk家族酪氨酸激酶与整联蛋白相互作用的试剂的方法。 还提供了使用这些试剂调节Syk家族的酪氨酸激酶并治疗受试者中的疾病如血栓形成，炎症，转移和肿瘤细胞生长的组合物和方法。

    Method for classification of anti-psychotic drugs
    42.
    发明申请
    Method for classification of anti-psychotic drugs 有权
    抗精神病药物分类方法

    公开(公告)号：US20030109419A1

    公开(公告)日：2003-06-12

    申请号：US10233448

    申请日：2002-09-03

    发明人： Paul Greengard ,  Gilberto Fisone

    IPC分类号： A61K031/00 ,  G01N033/53 ,  G01N033/567 ,  C12Q001/48

    CPC分类号： C12Q1/42 ,  C12Q1/485 ,  G01N33/6872 ,  G01N33/6896 ,  G01N33/94 ,  G01N33/9406 ,  G01N33/946 ,  G01N33/9466 ,  G01N33/9473 ,  G01N33/948 ,  G01N2500/00 ,  G01N2800/302

    摘要： The present invention provides a method for identifying an agent to be tested for an ability to treat a psychotic disorder in a patient in need of such treatment. The invention provides a method for screening candidate drugs for anti-psychotic drug activity, preferably atypical anti-psychotic activity, comprising contacting cells or tissues with a candidate drug, determining levels of phosphorylation of the intracellular signaling proteins, DARPP-32, ERK1, ERK2 and CREB, in said cells or tissues and determining the pattern of the levels of phosphorylation of the proteins. The pattern of the levels of phosphorylation of DARPP-32, ERK1, ERK2 and CREB is, in certain embodiments, compared with the pattern of the levels of phosphorylation of DARPP-32, ERK1, ERK2 and CREB in the presence of an atypical anti-psychotic drug.

    摘要翻译： 本发明提供了一种用于鉴定待测试试剂在治疗需要这种治疗的患者中治疗精神病的能力的方法。 本发明提供了用于筛选候选药物用于抗精神病药物活性的方法，优选非典型抗精神病活性，包括使候选药物接触细胞或组织，确定细胞内信号传导蛋白DARPP-32，ERK1，ERK2的磷酸化水平 和CREB，在所述细胞或组织中，并确定蛋白质的磷酸化水平的模式。 在某些实施方案中，DARPP-32，ERK1，ERK2和CREB的磷酸化水平的模式与DARPP-32，ERK1，ERK2和CREB的磷酸化水平的模式相比， 精神病药

    Molecules and methods using same for measuring non-transferrin bound iron
    44.
    发明申请
    Molecules and methods using same for measuring non-transferrin bound iron 有权
    分子和方法使用它来测量非转铁蛋白结合铁

    公开(公告)号：US20030104491A1

    公开(公告)日：2003-06-05

    申请号：US10283343

    申请日：2002-10-30

    发明人： Ioav Zvi Cabantchik ,  William Breuer ,  Breno P. Esposito

    IPC分类号： C12N009/10 ,  C12Q001/48 ,  G01N033/53

    CPC分类号： G01N33/90

    摘要： A molecule suitable for use as an indicator of free iron levels in a biological sample, the molecule including an iron binding moeity and a signal generating moeity, wherein an intensity of the signal generated by the signal generating moeity is related to an amount of the iron bound by the iron binding moeity.

    摘要翻译： 适合用作生物样品中游离铁含量指示剂的分子，该分子包括铁结合力和信号产生气味，其中由信号产生气味产生的信号的强度与铁的量有关 受铁结合力约束。

    Method for identifying inhibitors of dual substrate enzymes
    46.
    发明申请
    Method for identifying inhibitors of dual substrate enzymes 失效
    鉴定双重底物酶抑制剂的方法

    公开(公告)号：US20030049705A1

    公开(公告)日：2003-03-13

    申请号：US09682517

    申请日：2001-09-13

    发明人： Heidi Sue Dodson ,  James Scott Marks ,  Thomas John McQuade ,  Maxine Fico Santoro ,  Nicholas Santoro

    IPC分类号： C12Q001/48 ,  C12Q001/42

    CPC分类号： C12Q1/48 ,  C12Q1/42 ,  C12Q1/485

    摘要： A method for measuring the activity of dual substrate enzymes and for identifying compounds that modulate the activity of dual substrate enzymes comprising first substrate, a macromolecule that is enzymatically modified with a radioactively-labeled portion of the second substrate, said method comprising: a. adding a capture resin to a buffered mixture of an enzyme, allowing the enzyme to catalyze transfer of the radiolabeled portion of the radiolabeled second substrate to the non-radiolabeled first substrate, radiolabeled first substrate, and a radiolabeled second substrate in the presence or absence of a test compound; b. removing unreacted radiolabeled second substrate; c. adding a scintillant resin to the enzyme-radiolabeled first substrate mixture; and d. measuring the amount of radiolabeled first substrate by scintillation counting, measuring the amount of radiolabeled first substrate by scintillation counting, and comparing the two measurements.

    摘要翻译： 一种用于测量双重底物酶的活性并鉴定调节双重底物酶的活性的化合物的方法，所述化合物包含第一底物，用第二底物的放射性标记部分酶促修饰的大分子，所述方法包括：a。 向缓冲的酶混合物中加入捕获树脂，允许酶催化放射性标记的第二底物的放射性标记的部分转移到非放射性标记的第一底物，放射性标记的第一底物和放射性标记的第二底物，在存在或不存在 测试化合物 b。 去除未反应的放射性标记的第二底物; C。 向所述放射性标记的第一底物混合物中加入闪烁树脂; 和d。 通过闪烁计数测量放射性标记的第一底物的量，通过闪烁计数测量放射性标记的第一底物的量，并比较两次测量。

    Assay methods and kits therefor
    47.
    发明申请
    Assay methods and kits therefor 审中-公开
    测定方法和试剂盒

    公开(公告)号：US20030008338A1

    公开(公告)日：2003-01-09

    申请号：US10005189

    申请日：2001-12-07

    申请人： LumiTech (UK) Limited

    发明人： Sharon Patricia Mary Crouch ,  Kevin John Slater

    IPC分类号： C12Q001/66 ,  C12Q001/48

    CPC分类号： C12Q1/66 ,  C12Q1/485 ,  G01N33/5014

    摘要： The invention relates to methods for detecting the cytotoxic activity of an effector, such as a cytotoxic T lymphocyte, on a non-microbial target cell. Detection of cytotoxic activity is preferably achieved by detecting adenylate kinase release using photometric methods.

    摘要翻译： 本发明涉及用于检测效应物如细胞毒性T淋巴细胞在非微生物靶细胞上的细胞毒活性的方法。 细胞毒活性的检测优选通过使用光度法检测腺苷酸激酶释放来实现。

    Novel screening methods to identify agents that selectively inhibit hepatitis C virus replication
    48.
    发明申请
    Novel screening methods to identify agents that selectively inhibit hepatitis C virus replication 失效
    新型筛选方法来鉴定选择性抑制丙型肝炎病毒复制的药物

    公开(公告)号：US20020155431A1

    公开(公告)日：2002-10-24

    申请号：US09972756

    申请日：2001-10-05

    申请人： University of Washington

    发明人： Michael G. Katze ,  Michael J. Gale

    IPC分类号： C12Q001/70 ,  C12Q001/48

    CPC分类号： C07K14/005 ,  C12N9/1205 ,  C12N2770/24222 ,  C12Q1/48 ,  C12Q1/6897

    摘要： The present invention relates to novel methods for identifying antiviral agents which selectively interfere with viral proteins that override the interferon(IFN)-induced cellular defense mechanisms against viral infection. In particular, the present- invention relates to screening assays that identify agents which selectively inhibit the interaction between viral proteins containing an interferon sensitivity determining region (ISDR) and IFN-induced PKR protein kinase. The present invention more particularly relates to screening assays that identify agents which selectively inhibit the interaction between hepatitis C virus (HCV) nonstructural 5A protein (NS5A), which contains an ISDR, and IFN-induced PKR protein kinase. The interaction between the viral ISDR and IFN-induced PKR protein kinase results in the override of IFN-induced cellular defense mechanisms to combat viral infection. Therefore the agents identified using the assays of the invention may have utility as antiviral agents.

    摘要翻译： 本发明涉及用于鉴定选择性干扰超过干扰素（IFN）诱导的针对病毒感染的细胞防御机制的病毒蛋白的抗病毒剂的新方法。 特别地，本发明涉及鉴定选择性抑制含有干扰素敏感性决定区（ISDR）的病毒蛋白与IFN诱导的PKR蛋白激酶之间相互作用的试剂的筛选试验。 本发明更具体地涉及鉴定选择性抑制含有ISDR的丙型肝炎病毒（HCV）非结构5A蛋白（NS5A）与IFN诱导的PKR蛋白激酶之间相互作用的试剂的筛选试验。 病毒ISDR和IFN诱导的PKR蛋白激酶之间的相互作用导致IFN诱导的细胞防御机制的覆盖以对抗病毒感染。 因此，使用本发明的测定法鉴定的药剂可以用作抗病毒剂。

    Isolated FGF receptor
    49.
    发明申请
    Isolated FGF receptor 失效
    分离的FGF受体

    公开(公告)号：US20020090651A1

    公开(公告)日：2002-07-11

    申请号：US10016447

    申请日：2001-12-10

    申请人： President and Fellows of Harvard College

    发明人： Marc W. Kirschner ,  Noriyuki Kinoshita

    IPC分类号： G01N033/53 ,  C12Q001/68 ,  C12Q001/48 ,  C07H021/04 ,  C12N001/18 ,  C07K014/71

    CPC分类号： C07K14/46 ,  A61K38/00 ,  C07K14/705 ,  C07K16/18 ,  C12N15/1055 ,  C12N15/81

    摘要： Disclosed herein are compositions and methods which are useful in the identification and isolation of components involved in transmembrane receptor-mediated signaling. Such components include the receptors themselves (e.g., tyrosine kinase receptors, cytokine receptors and tyrosine phosphatase receptors), as well as ligands which bind the receptors and modulators of the downstream intracellular catalytic event which characterizes receptor-mediated signalling. Two novel ligands for the FGF receptor and the nucleotide sequences encoding them are also described.

    摘要翻译： 本文公开了可用于鉴定和分离参与跨膜受体介导的信号传导的成分的组合物和方法。 这些组分包括受体本身（例如酪氨酸激酶受体，细胞因子受体和酪氨酸磷酸酶受体）以及结合受体介导的信号传导的下游细胞内催化事件的受体和调节剂的配体。 还描述了用于FGF受体的两种新型配体和编码它们的核苷酸序列。