Bis-(polyhalohydrocarbyl) trisulfides as algicides
    41.
    发明授权
    Bis-(polyhalohydrocarbyl) trisulfides as algicides 失效
    BIS-(POLYHALOHYDROCARBYL)TRISULIFIDES as ALGICIDES

    公开(公告)号:US3694184A

    公开(公告)日:1972-09-26

    申请号:US3694184D

    申请日:1970-09-08

    申请人: CHEVRON RES

    发明人: KOHN GUSTAVE K

    IPC分类号: A01N41/12 A01H11/00 A01N9/12

    CPC分类号: A01N41/12

    摘要: BIS-(TETRAHALOETHYL) TRISULFIDES AND BIS-(TRIHALOVINYL) TRISULFIDES. THESE TRISULFIDES ARE PREPARED BY REACTING TETRAHALOETHYLSULFENYL CHLORIDE OR TRIHALOVINYLSULFENYL CHLORIDE, RESPECTIVELY, WITH HYDROGEN SULFIDE. THE TETRAHALOETHYL COMPOUNDS ARE USEFUL AS SEED DISINFECTANTS; WHEREAS THE TRIHALOVINYL MATERIALS ARE ALGICIDAL.

    Bio-pesticide and method for pest control
    43.
    发明授权
    Bio-pesticide and method for pest control 失效
    生物农药和害虫防治方法

    公开(公告)号:US08709399B2

    公开(公告)日:2014-04-29

    申请号:US13636143

    申请日:2011-03-24

    CPC分类号: A01N65/00 A01N63/04

    摘要: The present invention relates to a new method for pest control and/or for preventing or treating pest infestation. Said method comprises the inoculation of plants, parts of plants or the surrounding of said plants with an effective amount of endophytic Beauveria bassiana strains. In a further aspect the present invention relates to the use of an isolated Beauveria bassiana strain having superior properties. Furthermore, biopesticides and compositions for pest control, in particular, for control of herbivorous insects and/or plant pathogens, are provided.

    摘要翻译: 本发明涉及一种用于害虫防治和/或预防或治疗害虫侵害的新方法。 所述方法包括用有效量的内生白僵菌菌种接种植物,植物的部分或所述植物的周围。 在另一方面,本发明涉及具有优异性质的分离的白僵菌菌株的用途。 此外,还提供了用于病虫害防治的生物杀虫剂和组合物,特别是用于控制食草昆虫和/或植物病原体的生物杀虫剂和组合物。

    Methods for stratification and storage of somatic embryos
    45.
    发明授权
    Methods for stratification and storage of somatic embryos 有权
    体细胞胚分层和储存方法

    公开(公告)号:US08216840B2

    公开(公告)日:2012-07-10

    申请号:US12235460

    申请日:2008-09-22

    申请人: Amy M Jamruszka

    发明人: Amy M Jamruszka

    CPC分类号: A01H4/005

    摘要: In one aspect, a method is provided for producing stratified cotyledonary conifer somatic embryos. The method comprises (a) incubating a culture comprising immature conifer somatic embryos in a culture vessel comprising a development medium having an osmolality in the range of from 300 mM/Kg to 450 mM/Kg at a temperature of from 22° C. to 25° C. for a first incubation period sufficient in length for at least a portion of the embryos to reach anatomical maturity; and (b) subjecting the embryos in the culture vessel in accordance with step (a) to a temperature of from 0° C. to 10° C. for a second incubation period of at least one week to produce stratified cotyledonary somatic embryos.

    摘要翻译: 在一个方面,提供了一种用于产生分层的子叶针叶树体细胞胚的方法。 该方法包括(a)将包含未成熟针叶树体细胞胚的培养物在包含显影介质的培养容器中培养,所述显影培养基的重量克分子渗透浓度范围为300mM / Kg至450mM / Kg,温度为22℃至25℃ 对于至少一部分胚胎达到解剖成熟度的长度足够的第一潜伏期; 和(b)使根据步骤(a)的培养容器中的胚胎在0℃至10℃的温度下进行至少一周的第二次孵育期以产生分层的子叶体细胞胚。

    Systemic non-nodular endosymbiotic nitrogen fixation in plants
    46.
    发明授权
    Systemic non-nodular endosymbiotic nitrogen fixation in plants 有权
    系统性非结节性内共生植物固氮植物

    公开(公告)号:US08137665B2

    公开(公告)日:2012-03-20

    申请号:US12313592

    申请日:2008-11-21

    摘要: Non-leguminous crops, e.g. wheat, maize and rice, do not form nodules and are dependant for their nutrition on fixed nitrogen from the soil, or from chemical/nitrogenous fertilizers. The present invention provides non-leguminous plants and leguminous plants, including legumes that fail to nodulate with Rhizobia, with bacteria that enable them to fix nitrogen endophytically. Therefore, the plants contain nitrogen fixing bacteria the bacteria being located intracellularly in living plant cells.

    摘要翻译: 非豆科作物,例如 小麦,玉米和大米不会形成结节,并且依赖于在土壤或化学/氮肥肥料固定氮素上的营养。 本发明提供非豆科植物和豆科植物,包括不能根瘤菌结瘤的豆类,以及能够内生固氮的细菌。 因此,植物含有固氮细菌,细菌位于活细胞内细胞。

    Two Novel Alkylresorcinol Synthase Genes from Sorghum; Cloning, Expression, Transformation and Characterization
    47.
    发明申请
    Two Novel Alkylresorcinol Synthase Genes from Sorghum; Cloning, Expression, Transformation and Characterization 有权
    来自高粱的两种新型烷基间苯二酚合酶基因 克隆,表达,转化和表征

    公开(公告)号:US20110225676A1

    公开(公告)日:2011-09-15

    申请号:US12721206

    申请日:2010-03-10

    摘要: Sorghum is considered to be an allelopathic crop species, producing phytotoxins such as the lipid benzoquinone sorgoleone (2-hydroxy-5-methoxy-3-[(Z,Z)-8′,11′,14′-pentadecatriene]-p-benzoquinone) which likely accounts for much of its allelopathic properties. Prior investigations into the biosynthesis of sorgoleone have suggested the participation of one or more alkylresorcinol synthases (ARS), which are type III polyketide synthases (PKS) that produce 5-alkylresorcinols using medium to long-chain fatty acyl-CoA starter units via iterative condensations with malonyl-CoA. Current evidence suggests that sorgoleone biosynthesis occurs exclusively in root hair cells, involving the synthesis of a 5-pentadecatrienyl resorcinol intermediate derived from an unusual 16:3 fatty acyl-CoA starter unit. To characterize the enzymes responsible for the biosynthesis of this alkylresorcinol intermediate, a previously-described expressed sequence tag (EST) database prepared from isolated root hairs was first mined for all PKS-like sequences. Quantitative real-time RT-PCR analyses revealed that two of these sequences were preferentially expressed in root hairs, and recombinant enzyme studies demonstrated that both sequences (designated ARS1 and ARS2) encode ARS enzymes capable of accepting a variety of fatty acyl-CoA starter units. Furthermore, RNA interference (RNAi) experiments directed against ARS1 and ARS2 resulted in the generation of multiple independent transformant events exhibiting dramatically reduced sorgoleone levels. Thus, both ARS1 and ARS2 are likely to participate in the biosynthesis of sorgoleone in planta. The sequences of ARS1 and ARS2 were also used to identify several rice genes encoding ARSs, which are likely involved in the production of defense-related alkylresorcinols.

    摘要翻译: 高粱被认为是一种化感作物,产生植物毒素,如脂质苯醌山梨酮(2-羟基-5-甲氧基-3 - [(Z,Z)-8',11',14'-十五碳烯] 苯醌),其可能占据其大部分的抗病性。 对sorgoleone的生物合成的先前研究表明,一种或多种烷基间苯二酚合酶(ARS)的参与,其是使用中等至长链脂肪酰辅酶A起始单元通过迭代缩合产生5-烷基间苯二酚的III型聚酮化合物合酶(PKS) 与丙二酰辅酶A。 目前的证据表明,sorgoleone生物合成仅在根毛细胞中发生,涉及合成来自不寻常的16:3脂肪酰辅酶A启动子单元的5-十五碳烯基间苯二酚中间体。 为了表征负责该烷基间苯二酚中间体的生物合成的酶,首先从所有PKS样序列中分离由分离的根毛制备的先前描述的表达序列标签(EST)数据库。 定量实时RT-PCR分析显示,这些序列中的两个优先在根毛中表达,重组酶研究表明两个序列(指定为ARS1和ARS2)编码能够接受各种脂肪酰辅酶A起始单位的ARS酶 。 此外,针对ARS1和ARS2的RNA干扰(RNAi)实验导致产生显着降低的sorgoleone水平的多个独立转化体事件。 因此,ARS1和ARS2都可能参与植物中sorgoleone的生物合成。 ARS1和ARS2的序列也用于鉴定编码ARS的几个水稻基因,其可能参与防御相关的烷基间苯二酚的生产。

    Cis-prenyltransferases from plants
    50.
    发明授权
    Cis-prenyltransferases from plants 有权
    来自植物的顺式异戊烯基转移酶

    公开(公告)号:US07880058B2

    公开(公告)日:2011-02-01

    申请号:US11843676

    申请日:2007-08-23

    IPC分类号: A01H11/00 C07H21/04

    摘要: This invention pertains to nucleic acid fragments encoding plant proteins that are homologs to the cis-prenyltransferases UPP synthase from the bacterium Micrococcus luteus or Dedol-PP synthase from yeast Saccharomyces cerevisiae. More specifically, this invention pertains to cis-prenyltransferase homologs from wheat, grape, soybean, rice, African daisy, rubber tree latex and pot marigold.

    摘要翻译: 本发明涉及编码植物蛋白质的核酸片段,其与来自酵母酿酒酵母的细菌微球菌或Dedol-PP合酶的顺式 - 异戊烯基转移酶UPP合酶的同系物。 更具体地说,本发明涉及来自小麦,葡萄,大豆,大米,非洲雏菊,橡胶树乳胶和万寿菊的顺式 - 异戊烯基转移酶同源物。