公开(公告)号：US10266864B2

公开(公告)日：2019-04-23

申请号：US14773696

申请日：2014-03-10

Applicant: BASF ENZYMES LLC

Inventor： Adrienne Huston Davenport ,  Hugo D. Urbina ,  Kenneth E. Barrett ,  Danielle Cusumano

IPC: C12P19/16 ,  C12P19/02 ,  C12N9/44

Abstract: The present invention relates to thermostable pullulanases useful for industrial and scientific purposes. The present invention provides methods for producing the modified pullulanase, enzymatic compositions comprising the modified pullulanase, and methods for use of the enzymatic compositions.

公开(公告)号：US20180312744A1

公开(公告)日：2018-11-01

申请号：US15835386

申请日：2017-12-07

Applicant: BASF Enzymes, LLC

Inventor： Xuqiu Tan ,  Kenneth E. Barrett ,  Adrienne Huston Davenport ,  Lawrence E. Whipple ,  Hugo D. Urbina ,  Bin Zhang ,  Mark A. Wall

IPC: C09K8/68 ,  C12P19/04 ,  C12N9/42 ,  C12P19/14

CPC classification number: C09K8/68 ,  C09K2208/24 ,  C12N9/2437 ,  C12P19/04 ,  C12P19/14

Abstract: Polynucleotide sequences encoding a thermostable cellulase and directing its increased expression are provided, and hydraulic fracturing compositions comprising such thermostable cellulase.

公开(公告)号：US20180258410A1

公开(公告)日：2018-09-13

申请号：US15879379

申请日：2018-01-24

Applicant: BASF Enzymes LLC

Inventor： Xuqiu Tan ,  Arne I. Solbak, JR.

IPC: C12N9/16

Abstract: Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/L to 40 g/L. The phytase have higher specific activity, retain activity at low pH, and retain activity at high temperature. The phytase can be used in a variety of compositions including food, feed, pharmaceuticals, and cleaning.

公开(公告)号：US10017754B2

公开(公告)日：2018-07-10

申请号：US14389339

申请日：2013-03-12

Applicant: BASF Enzymes LLC

Inventor： Xuqiu Tan ,  Kenneth E. Barrett ,  Richard S. Lee

IPC: C12N9/42 ,  C12N9/24 ,  C09K8/52 ,  C09K8/68 ,  C11D3/386 ,  C12N9/26

CPC classification number: C12N9/2437 ,  C09K8/52 ,  C09K8/68 ,  C09K2208/24 ,  C11D3/38645 ,  C12N9/2411

Abstract: Polynucleotide sequences are provided encoding a thermostable cellulase and directing its increased expression are provided, and the use of the thermostable cellulase in hydraulic fracturing methods and the treatment of flowback fluids.

公开(公告)号：US20170009215A1

公开(公告)日：2017-01-12

申请号：US15186160

申请日：2016-06-17

Applicant: BASF ENZYMES LLC

Inventor： DAVID P. WEINER ,  PETER LUGINBUHL ,  ANALIA BUENO ,  JOSLIN M. CUENCA ,  ERIN MARASCO

IPC: C12N9/10 ,  C12N15/86 ,  C12Q1/68

CPC classification number: C12N9/1096 ,  A21D8/042 ,  A61K38/00 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0014 ,  C12N9/10 ,  C12N15/1137 ,  C12N15/63 ,  C12N15/86 ,  C12P7/10 ,  C12Q1/6876 ,  C12Y206/01021 ,  D06L4/40 ,  D06M16/003 ,  G01N33/573 ,  Y02E50/16 ,  Y02P20/52 ,  Y02T50/678

Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., d-amino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine+2-oxoglutarate pyruvate+D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid+H2O+acceptor a 2-oxo acid+NH3+reduced acceptor.

Abstract translation: 本发明一般涉及酶，编码酶的多核苷酸，这种多核苷酸和多肽的用途，更具体地涉及具有转移酶活性的酶，例如转氨酶活性，例如d-氨基酸转移酶活性和/或氧化还原酶活性，例如 ，脱氢酶活性，例如，d-氨基酸脱氢酶活性和/或催化化学基团的转移，催化转氨酶，催化反应：D-丙氨酸+ 2-氧戊二酸酯<=>丙酮酸盐+ D-谷氨酸盐和/ 或催化氧化还原反应，催化氢原子的去除和/或催化反应：D-氨基酸+ H 2 O +受体<=> 2-氧代酸+ NH 3 +还原受体。

公开(公告)号：US20160355801A1

公开(公告)日：2016-12-08

申请号：US15064269

申请日：2016-03-08

Applicant: BASF ENZYMES LLC

Inventor： KELVIN WONG ,  JAY M. SHORT ,  MARK J. BURK ,  GRACE DESANTIS ,  ROBERT FARWELL ,  KELLY CHATMAN

IPC: C12N9/78 ,  C12P7/42 ,  C12P41/00 ,  C12P13/00

CPC classification number: C12N9/78 ,  C12N15/00 ,  C12P7/42 ,  C12P13/002 ,  C12P13/04 ,  C12P41/006 ,  C12Y305/05001 ,  Y02P20/52

Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

公开(公告)号：US20160251643A1

公开(公告)日：2016-09-01

申请号：US15009414

申请日：2016-01-28

Applicant: BASF Enzymes LLC

Inventor： David P. WEINER ,  Lishan ZHAO ,  Peter LUGINBUHL ,  Ellen BURKE ,  Toby H. RICHARDSON ,  Paula M. HICKS

IPC: C12N9/88

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

Abstract translation: 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。

公开(公告)号：US20160237411A1

公开(公告)日：2016-08-18

申请号：US15141467

申请日：2016-04-28

Applicant: BASF ENZYMES LLC ,  BASF SE

Inventor： JANNE SAMULI KEROVUO ,  SYLKE HAREMZA ,  OLIVER KOCH ,  TILO HABICHER ,  DAN E. ROBERTSON ,  GRACE DESANTIS ,  RYAN MCCANN ,  PETER LUGINBUHL

IPC: C12N9/02

CPC classification number: C12N9/0061 ,  C12N15/09 ,  C12P5/00 ,  C12P7/00 ,  C12P19/00 ,  C12Y110/03002 ,  D21C5/005 ,  D21C9/005 ,  D21C9/1063

Abstract: Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins, other phenolic, and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.

Abstract translation: 本文提供了分离的漆酶和编码它们的核酸。 还提供了漆酶反应的介质。 本文还提供了使用漆酶来氧化木质素，其它酚类和芳族化合物的方法，例如用于在高温和pH条件下木浆的生物漂白和脱色以促进漂白化学品的使用的显着减少，以及 用于治疗纤维。

公开(公告)号：US09353477B2

公开(公告)日：2016-05-31

申请号：US14591449

申请日：2015-01-07

Applicant: BASF ENZYMES LLC ,  BASF SE

Inventor： Janne Samuli Kerovuo ,  Sylke Haremza ,  Oliver Koch ,  Tilo Habicher ,  Dan E. Robertson ,  Grace Desantis ,  Ryan McCann ,  Peter Luginbuhl

IPC: C12N9/02 ,  C12N15/00 ,  C12P19/00 ,  C12P7/00 ,  C12P5/00 ,  C07H21/04 ,  D21C9/00 ,  C12N15/09 ,  D21C9/10 ,  D21C5/00

CPC classification number: C12N9/0061 ,  C12N15/09 ,  C12P5/00 ,  C12P7/00 ,  C12P19/00 ,  C12Y110/03002 ,  D21C5/005 ,  D21C9/005 ,  D21C9/1063

Abstract: Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.

公开(公告)号：US09315792B2

公开(公告)日：2016-04-19

申请号：US14297989

申请日：2014-06-06

Applicant: BASF ENZYMES LLC

Inventor： Kelvin Wong ,  Jay M. Short ,  Mark J. Burk ,  Grace DeSantis ,  Robert Farwell ,  Kelly Chatman

IPC: C12N9/78 ,  C12N15/00 ,  C12P21/06 ,  C12P21/04 ,  C07H21/04 ,  C12P7/42 ,  C12P13/04 ,  C12P41/00

CPC classification number: C12N9/78 ,  C12N15/00 ,  C12P7/42 ,  C12P13/002 ,  C12P13/04 ,  C12P41/006 ,  C12Y305/05001 ,  Y02P20/52

Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.