公开(公告)号：US20220251668A1

公开(公告)日：2022-08-11

申请号：US17728789

申请日：2022-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Yi Zhou ,  Saedeh Sepehri ,  Christopher Siemer

IPC: C12Q1/6886 ,  C12Q1/6841 ,  G01N33/574 ,  B01L3/00 ,  C12Q1/6834

Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.

公开(公告)号：US11377684B2

公开(公告)日：2022-07-05

申请号：US15973243

申请日：2018-05-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Duc Do ,  Claudia Litterst ,  Dianna Maar

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6844 ,  G01N21/64

Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

公开(公告)号：US20220195484A1

公开(公告)日：2022-06-23

申请号：US17686466

申请日：2022-03-04

Applicant: BIO-RAD EUROPE GMBH ,  BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHE QUIRING ,  CHRISTINE FAVIER ,  PATRICE SARFATI ,  JEAN FRANCOIS MOUSCADET ,  RONALD LEBOFSKY ,  REBECCA DIEVART

IPC: C12Q1/06 ,  C12Q1/04 ,  C12N9/34 ,  C12N9/42 ,  C12N9/40 ,  C12N9/38 ,  C12N9/26 ,  C12N9/48

Abstract: Methods, compositions, and kits are provided for rapidly analyzing microbial growth and/or number in a plurality of water-in-oil emulsion droplets.

公开(公告)号：US20220187191A1

公开(公告)日：2022-06-16

申请号：US17686229

申请日：2022-03-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  G01N1/28 ,  G01N1/40 ,  G01N1/20

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US20220154266A1

公开(公告)日：2022-05-19

申请号：US17665170

申请日：2022-02-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yi XUE ,  Joshua RITCHEY ,  Robert MELTZER

IPC: C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6844

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

公开(公告)号：US20220152606A1

公开(公告)日：2022-05-19

申请号：US17590543

申请日：2022-02-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC: B01L3/00 ,  C12N13/00

Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US20220119896A1

公开(公告)日：2022-04-21

申请号：US17507472

申请日：2021-10-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Monica HERRERA ,  Josh SHINOFF

IPC: C12Q1/70 ,  C12Q1/689 ,  C12Q1/6895

Abstract: Methods of concentrating RNA from wastewater and quantifying the RNA by digital amplification are provided.

公开(公告)号：US11273439B2

公开(公告)日：2022-03-15

申请号：US16906337

申请日：2020-06-19

Applicant: Bio-Rad Laboratories, Inc

Inventor： Kalyan Handique ,  Swati Ranade ,  Vishal Sharma ,  Austin Payne ,  Sam Tuck

IPC: B01L3/00 ,  C12N13/00

Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).

公开(公告)号：US20220064711A1

公开(公告)日：2022-03-03

申请号：US17529082

申请日：2021-11-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6806 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12N15/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US11262366B2

公开(公告)日：2022-03-01

申请号：US16145021

申请日：2018-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Roger Walker ,  Benedicte Jardin

IPC: G01N33/531 ,  C07K16/18 ,  G01N33/72 ,  G01N33/58

Abstract: Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided.