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公开(公告)号:US20190031752A1
公开(公告)日:2019-01-31
申请号:US15898286
申请日:2018-02-16
Applicant: UCB PHARMA S.A.
Inventor: Paul Edward Stephens , Michael John Wright
IPC: C07K16/24
Abstract: This invention provides a method for obtaining a recombinant antibody with a desired function, comprising: (a) providing a population of anti-body-forming cells suspected of containing at least one cell capable of producing an antibody exhibiting the desired function; (b) generating one or more transcriptionally active recombinant linear polynucleotides from the antibody-forming cells obtained in step (a) wherein each transcriptionally active recombinant linear polynucleotide comprises a polynucleotide sequence encoding a variable domain of an antibody produced by an antibody-forming cell obtained in step (a) and one or more transcription regulatory elements; (c) expressing a recombinant antibody using one or more of the transcriptionally active recombinant linear polynucleotides generated in step (b); (d) screening the recombinant antibody produced by step (c) for the desired function; and (e) optionally repeating steps (b), (c) and (d) to identify a recombinant antibody exhibiting the desired function.
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公开(公告)号:US09902768B2
公开(公告)日:2018-02-27
申请号:US14380310
申请日:2013-02-22
Applicant: UCB Pharma S.A.
Inventor: David Paul Humphreys , Shirley Jane Peters
CPC classification number: C07K16/24 , C07K16/00 , C07K16/468 , C07K2317/31 , C07K2317/51 , C07K2317/522 , C07K2317/53 , C07K2317/55 , C07K2317/94
Abstract: The present disclosure provides an asymmetric mixed antibody comprising two heavy chains or heavy chain fragments each comprising at least a variable region, a hinge region and a CH1 domain, wherein a first heavy chain or fragment thereof is a class IgG4 and has: a the inter-chain cysteine at position 127, numbered according to the Kabat numbering system, in the CH1 domain is substituted with another amino acid; and b optionally one or more of the amino acids positioned in the upper hinge region is substituted with cysteine, and wherein the second heavy chain or fragment thereof is characterized in that part or all of the chain has a different amino acid sequence to said first heavy chain in at least the region outside the variable region (for example the constant region), formulations comprising the same, therapeutic used of both of the above, and processes for preparing the antibodies and formulation.
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公开(公告)号:US09828438B2
公开(公告)日:2017-11-28
申请号:US15058460
申请日:2016-07-06
Applicant: UCB PHARMA S.A.
Inventor: David Paul Humphreys , Emma Dave , Laura Griffin , Sam Philip Heywood
CPC classification number: C07K16/468 , C07K16/00 , C07K16/18 , C07K16/245 , C07K2317/24 , C07K2317/31 , C07K2317/522 , C07K2317/54 , C07K2317/55 , C07K2317/569 , C07K2317/64 , C07K2317/92
Abstract: The present invention provides dual specificity antibody fusion proteins comprising an antibody Fab or Fab′ fragment with specificity for an antigen of interest, said fragment being fused to at least one single domain antibody which has specificity for a second antigen of interest.
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公开(公告)号:US20170335003A1
公开(公告)日:2017-11-23
申请号:US15667649
申请日:2017-08-03
Applicant: UCB PHARMA, S.A.
Inventor: MARK ELLIS , DAVID PAUL HUMPHREYS
CPC classification number: C07K16/2875 , C07K2317/14 , C07K2317/55 , C12N9/90
Abstract: The present invention provides a recombinant gram-negative bacterial cell comprising an expression vector comprising a recombinant polynucleotide encoding DsbC and one or more polynucleotides encoding an antibody or an antigen-binding fragment thereof specifically binding to CD154.
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公开(公告)号:US09550973B2
公开(公告)日:2017-01-24
申请号:US15083446
申请日:2016-03-29
Applicant: UCB PHARMA, S.A.
Inventor: Mark Ellis , David Paul Humphreys
CPC classification number: C12N1/20 , C07K16/241 , C07K16/244 , C07K2317/14 , C07K2317/20 , C07K2317/55 , C12N9/52 , C12N15/70 , C12P21/02 , C12R1/19 , Y02P20/52
Abstract: A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.
Abstract translation: 包含一个或多个以下突变蛋白酶基因的重组革兰氏阴性细菌细胞:a)突变的Tsp基因,其中突变的Tsp基因编码具有降低的蛋白酶活性的Tsp蛋白或是敲除突变的Tsp基因; b)突变的ptr基因,其中突变的ptr基因编码具有降低的蛋白酶活性的蛋白酶III蛋白质或是敲除突变的ptr基因; 和c)编码具有伴侣活性和降低的蛋白酶活性的DegP蛋白的突变DegP基因; 其中所述细胞与野生型细菌细胞是同基因的,除了突变的Tsp基因和/或突变的ptr基因和/或突变的DegP基因和任选的编码目的蛋白质的多核苷酸序列。
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公开(公告)号:US20160355598A1
公开(公告)日:2016-12-08
申请号:US15242135
申请日:2016-08-19
Applicant: UCB Pharma S.A.
Inventor: Alastair David Griffiths Lawson , Andrew Malcolm Nesbitt , Andrew George Popplewell , Stevan Graham Shaw , Diana Shpektor , Yi Zhang
IPC: C07K16/28
CPC classification number: C07K16/2878 , A61K39/3955 , A61K45/06 , A61K2039/505 , C07K14/70578 , C07K2317/24 , C07K2317/33 , C07K2317/55 , C07K2317/565 , C07K2317/73 , C07K2317/76 , C07K2317/92
Abstract: The invention relates to antibody molecules having specificity for antigenic determinants of human OX40, therapeutic uses of the antibody molecules and methods for producing said antibody molecules.
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57.
公开(公告)号:US20160187357A1
公开(公告)日:2016-06-30
申请号:US14626274
申请日:2015-02-19
Applicant: UCB PHARMA S.A.
Inventor: Mary E. Brunkow , David J. Galas , Brian Kovacevich , John T. Mulligan , Bryan W. Paeper , Jeffrey Van Ness , David G. Winkler
IPC: G01N33/74
CPC classification number: G01N33/74 , A01K2217/05 , A01K2217/075 , A61K38/18 , A61K39/3955 , A61K45/06 , A61K48/00 , C07K14/475 , C07K14/51 , C07K16/18 , C07K16/22 , C07K2317/11 , C07K2317/23 , C07K2319/00 , C12N2799/021 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/172 , G01N33/6872 , G01N33/84 , G01N2333/47 , G01N2333/495 , G01N2333/51 , G01N2500/02 , G01N2500/20
Abstract: A novel class or family of TGF-β binding proteins is disclosed. Also disclosed are assays for selecting molecules for increasing bone mineralization and methods for utilizing such molecules.
Abstract translation: 一种新型的TGF- 公开了结合蛋白。 还公开了用于选择用于增加骨矿化的分子的测定法和利用这种分子的方法。
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公开(公告)号:US20160185761A1
公开(公告)日:2016-06-30
申请号:US14909246
申请日:2014-07-28
Applicant: UCB PHARMA, S.A.
Inventor: Florian Montel , Eric Jnoff
IPC: C07D403/06
CPC classification number: C07D403/06
Abstract: The invention relates to 2-oxo-1-pyrrolidinyl triazole derivatives, processes for preparing them, pharmaceutical compositions containing them and their use as pharmaceuticals for enhancing the cognitive function or to counteract cognitive decline.
Abstract translation: 本发明涉及2-氧代-1-吡咯烷基三唑衍生物,其制备方法,含有它们的药物组合物及其作为用于增强认知功能或抵抗认知衰退的药物的用途。
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公开(公告)号:US20160108119A1
公开(公告)日:2016-04-21
申请号:US14977688
申请日:2015-12-22
Applicant: UCB PHARMA, S.A.
CPC classification number: C07K16/241 , C07K1/18 , C07K1/36 , C07K2317/14 , C07K2317/55
Abstract: The present invention relates to a process for the purification of an antibody fragment from a periplasmic cell extract comprising a first cation exchange chromatography step and a second anion exchange chromatography step.
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公开(公告)号:US20150344840A1
公开(公告)日:2015-12-03
申请号:US14827408
申请日:2015-08-17
Applicant: UCB PHARMA, S.A.
Inventor: MARK ELLIS , DAVID PAUL HUMPHREYS
CPC classification number: C12N1/20 , C07K16/241 , C07K16/244 , C07K2317/14 , C07K2317/20 , C07K2317/55 , C12N9/52 , C12N15/70 , C12P21/02 , C12R1/19 , Y02P20/52
Abstract: A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.
Abstract translation: 包含一个或多个以下突变蛋白酶基因的重组革兰氏阴性细菌细胞:a)突变的Tsp基因,其中突变的Tsp基因编码具有降低的蛋白酶活性的Tsp蛋白或是敲除突变的Tsp基因; b)突变的ptr基因,其中突变的ptr基因编码具有降低的蛋白酶活性的蛋白酶III蛋白质或是敲除突变的ptr基因; 和c)编码具有伴侣活性和降低的蛋白酶活性的DegP蛋白的突变DegP基因; 其中所述细胞与野生型细菌细胞是同基因的,除了突变的Tsp基因和/或突变的ptr基因和/或突变的DegP基因和任选的编码目的蛋白质的多核苷酸序列。
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