Kits for measuring cellular transmembrane potential changes
    51.
    发明申请
    Kits for measuring cellular transmembrane potential changes 有权
    用于测量细胞跨膜电位变化的试剂盒

    公开(公告)号:US20050153275A1

    公开(公告)日:2005-07-14

    申请号:US11020536

    申请日:2004-12-22

    CPC分类号: G01N33/52 Y10S435/975

    摘要: The invention encompasses kits for an improved method for measuring membrane potential using compounds of the formula I as potentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPR™, fluorescent imaging plate reader, sold by Molecular Devices Corp., of Sunnyvale, Calif.; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells.

    摘要翻译: 本发明包括用于使用式I化合物作为电位探针测量膜电位的改进方法的试剂盒。 这些探针可以与其它荧光指示剂如Indo-1,Fura-2和Fluo-3组合使用,这样的探针可以用于微孔板读取装置,例如FLIPR TM,荧光成像板读数器,由Molecular 设备公司,加利福尼亚州桑尼维尔市 流式细胞仪 和荧光计。 这种探针用于测量活细胞中的膜电位。

    Methods of measuring luminogenic activity with a protected coelenterazine
    57.
    发明授权
    Methods of measuring luminogenic activity with a protected coelenterazine 有权
    用保护的肠培养液测量发光活性的方法

    公开(公告)号:US07537912B2

    公开(公告)日:2009-05-26

    申请号:US11832169

    申请日:2007-08-01

    IPC分类号: C12Q1/44

    摘要: A method of measuring the enzymatic activity of a luciferase includes contacting a luminogenic protein, such as a luciferase, with a protected luminophore to form a composition; and detecting light produced from the composition. The protected luminophore provides increased stability and improved signal-to-background ratios relative to the corresponding unmodified coelenterazine.

    摘要翻译: 测量荧光素酶的酶活性的方法包括将发光蛋白如荧光素酶与受保护的发光体接触以形成组合物; 并检测由该组合物产生的光。 受保护的发光体相对于相应的未修饰的肠培养基提供增加的稳定性和改善的信号与背景比。

    Kits for measuring cellular transmembrane potential changes
    58.
    发明授权
    Kits for measuring cellular transmembrane potential changes 有权
    用于测量细胞跨膜电位变化的试剂盒

    公开(公告)号:US07358064B2

    公开(公告)日:2008-04-15

    申请号:US11020536

    申请日:2004-12-22

    IPC分类号: C12Q1/02

    CPC分类号: G01N33/52 Y10S435/975

    摘要: The invention encompasses kits for an improved method for measuring membrane potential using compounds of the formula I as potentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPR™, fluorescent imaging plate reader, sold by Molecular Devices Corp., of Sunnyvale, Calif.; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells wherein X is O or S; and n is 1 or 2.

    摘要翻译: 本发明包括用于使用式I化合物作为电位探针测量膜电位的改进方法的试剂盒。 这些探针可以与其它荧光指示剂如Indo-1,Fura-2和Fluo-3组合使用,这样的探针可以用于微孔板读取装置,例如FLIPR TM,荧光成像板读数器,由Molecular 设备公司,加利福尼亚州桑尼维尔市 流式细胞仪 和荧光计。 这样的探针用于测量活细胞中的膜电位,其中X是O或S; n为1或2。

    Method for measuring cellular transmembrane potential changes
    59.
    发明授权
    Method for measuring cellular transmembrane potential changes 有权
    测量细胞跨膜电位变化的方法

    公开(公告)号:US06852504B2

    公开(公告)日:2005-02-08

    申请号:US09924797

    申请日:2001-08-08

    IPC分类号: G01N33/52 C12Q1/02

    CPC分类号: G01N33/52 Y10S435/975

    摘要: The invention encompasses an improved method for measuring membrane potential using compounds of the formula I as potentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPR™, fluorescent imaging plate reader, sold by Molecular Device Corp., of Sunnyvale, Calif.; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells

    摘要翻译: 本发明包括使用式I化合物作为电位探针来测量膜电位的改进方法。 这些探针可以与其它荧光指示剂如Indo-1,Fura-2和Fluo-3组合使用,这样的探针可以用于微孔板读取装置,例如FLIPR TM,荧光成像板读数器,由Molecular Device Corp.,Sunnyvale,Calif。 流式细胞仪 和荧光计。 这种探针用于测量活细胞中的膜电位