利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

60 条记录 (耗时 0.052 秒)

    Reactive cyanine compounds
    3.
    发明授权
    Reactive cyanine compounds 有权
    活性花青化合物

    公开(公告)号：US08309059B2

    公开(公告)日：2012-11-13

    申请号：US12872467

    申请日：2010-08-31

    申请人： Cesear Corona ,  Dieter Klaubert ,  Mark McDougall

    发明人： Cesear Corona ,  Dieter Klaubert ,  Mark McDougall

    IPC分类号： A61B5/00 ,  A61B8/00 ,  A61B10/00 ,  C07D209/02

    CPC分类号： G01N33/582 ,  A61K49/0032 ,  A61K49/0056 ,  C09B23/0008 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083

    摘要： The invention provides compounds and compositions of Formulas I-VII, and methods of using the compounds. The compounds can be used to prepare dye conjugates that are uniformly and substantially more fluorescent on proteins, nucleic acids or other biopolymers, than conjugates labeled with structurally similar known carbocyanine dyes. In addition to having more intense fluorescence emission than structurally similar dyes at virtually identical wavelengths, and decreased artifacts in their absorption spectra upon conjugation to biopolymers, the compounds can have greater photostability and/or higher absorbance (extinction coefficients) at the wavelength(s) of peak absorbance than such structurally similar dyes.

    摘要翻译： 本发明提供式I-VII的化合物和组合物，以及使用该化合物的方法。 该化合物可用于制备在蛋白质，核酸或其他生物聚合物上均匀且基本上更多荧光的染料缀合物，而不是与结构上相似的已知碳菁染料标记的缀合物。 除了与实际相同的波长下的结构相似的染料具有更强烈的荧光发射以及在与生物聚合物共轭时其吸收光谱中的伪影减少，化合物在波长处具有更大的光稳定性和/或更高的吸光度（消光系数） 的吸光度比这种结构相似的染料高。

    LUMINESCENCE-BASED METHODS AND PROBES FOR MEASURING CYTOCHROME P450 ACTIVITY
    6.
    发明申请
    LUMINESCENCE-BASED METHODS AND PROBES FOR MEASURING CYTOCHROME P450 ACTIVITY 有权
    用于测量细胞色素P450活性的基于荧光的方法和探针

    公开(公告)号：US20110003316A1

    公开(公告)日：2011-01-06

    申请号：US12754164

    申请日：2010-04-05

    申请人： James J. Cali ,  Dieter Klaubert ,  William Daily ,  Samuel Kin Sang Ho ,  Susan Frackman ,  Erika Hawkins ,  Keith V. Wood

    发明人： James J. Cali ,  Dieter Klaubert ,  William Daily ,  Samuel Kin Sang Ho ,  Susan Frackman ,  Erika Hawkins ,  Keith V. Wood

    IPC分类号： G01N33/53 ,  C12Q1/66

    CPC分类号： G01N33/502 ,  A61K49/0008 ,  C07D417/04 ,  C12Q1/66 ,  G01N33/5088 ,  G01N33/582 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2500/10

    摘要： The present invention provides methods, compositions, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferin or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. Upon addition of the luciferin derivative or other luminogenic molecule into a P450 reaction, the P450 enzyme metabolizes the molecule into a bioluminescent enzyme substrate, e.g., luciferin and/or luciferin derivative metabolite, in a P450 reaction. The resulting metabolite(s) serves as a substrate of the bioluminescent enzyme, e.g., luciferase, in a second light-generating reaction. Luminescent cytochrome P450 assays with low background signals and high sensitivity are disclosed and isoform selectivity is demonstrated. The present invention also provides an improved method for performing luciferase reactions which employs added pyrophosphatase to remove inorganic pyrophosphate, a luciferase inhibitor which may be present in the reaction mixture as a contaminant or may be generated during the reaction. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors.

    摘要翻译： 本发明提供了可用于分析细胞，组织和动物中的代谢活性以及筛选测试化合物对细胞色素P450活性的影响的方法，组合物，底物和试剂盒。 特别地，使用发光分子的一步和两步法。 荧光素或coelenterazines，其是细胞色素P450底物并且也是生物发光酶，例如萤光素酶，原底物。 在P450反应中加入荧光素衍生物或其他发光分子时，P450酶在P450反应中将分子代谢为生物发光酶底物，例如荧光素和/或荧光素衍生物代谢产物。 所产生的代谢物在第二次发光反应中用作生物发光酶，例如荧光素酶的底物。 公开了具有低背景信号和高灵敏度的发光细胞色素P450测定，证明了同工型选择性。 本发明还提供一种用于进行荧光素酶反应的改进方法，其使用添加的焦磷酸酶来除去无机焦磷酸盐，荧光素酶抑制剂，其可作为污染物存在于反应混合物中，或可能在反应期间产生。 本发明方法还提供了一种使用荧光素酶稳定剂如可逆荧光素酶抑制剂在基于荧光素酶的测定中稳定和延长发光信号的方法。

    METHODS TO QUENCH LIGHT FROM OPTICAL REACTIONS
    8.
    发明申请
    METHODS TO QUENCH LIGHT FROM OPTICAL REACTIONS 审中-公开
    从光学反应中排除光的方法

    公开(公告)号：US20080248511A1

    公开(公告)日：2008-10-09

    申请号：US12056073

    申请日：2008-03-26

    申请人： William Daily ,  Erika Hawkins ,  Dieter Klaubert ,  Mark McDougall ,  James Unch ,  Keith V. Wood ,  Wenhui Zhou ,  Ji Zhu

    发明人： William Daily ,  Erika Hawkins ,  Dieter Klaubert ,  Mark McDougall ,  James Unch ,  Keith V. Wood ,  Wenhui Zhou ,  Ji Zhu

    IPC分类号： C07D209/16 ,  C12Q1/66

    CPC分类号： C12Q1/66

    摘要： The present invention relates to single and dual reporter luminescence assays utilizing reagents to quench an optical, e.g., an enzyme-mediated luminescence, reaction. In one embodiment of the invention, a reagent is added to an assay which selectively quenches a first enzyme-mediated luminescence reaction without affecting a subsequent distinct enzyme-mediated luminescent reaction(s). An assay kit containing one or more selective quench reagents, and compositions comprising the quench reagent(s), are also provided.

    摘要翻译： 本发明涉及使用试剂淬灭光学例如酶介导的发光反应的单和双重报道发光测定。 在本发明的一个实施方案中，将试剂加入到选择性淬灭第一酶介导的发光反应而不影响随后不同的酶介导的发光反应的测定中。 还提供了含有一种或多种选择性骤冷试剂和包含骤冷试剂的组合物的测定试剂盒。