公开(公告)号：US07189503B2

公开(公告)日：2007-03-13

申请号：US10739585

申请日：2003-12-18

申请人： Mark Akeson ,  Daniel Branton ,  George Church ,  David W. Deamer

发明人： Mark Akeson ,  Daniel Branton ,  George Church ,  David W. Deamer

IPC分类号： G01N33/483

CPC分类号： G01N33/48721 ,  B82Y15/00 ,  B82Y30/00 ,  C07K14/705 ,  C12N9/1241 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6869 ,  G01N33/68 ,  Y10S977/795 ,  Y10S977/88 ,  Y10S977/953 ,  Y10S977/957 ,  Y10S977/958 ,  C12Q2565/631 ,  C12Q2523/307 ,  C12Q2565/607

摘要： The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.

公开(公告)号：US20120160687A1

公开(公告)日：2012-06-28

申请号：US13186966

申请日：2011-07-20

申请人： Mark Akeson ,  Daniel Branton ,  George Church ,  David W. Deamer

发明人： Mark Akeson ,  Daniel Branton ,  George Church ,  David W. Deamer

IPC分类号： C07K1/26

CPC分类号： C12Q1/6869 ,  G01N27/447 ,  G01N33/48721 ,  C12Q2565/631 ,  C12Q2565/607

摘要： The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.

摘要翻译： 本发明涉及一种用于通过（1）在两个池之间提供两个独立的相邻的介质池和界面来检测核酸中的双链区域的方法，所述界面具有如此尺寸以允许顺序单体 单链核酸但不是双链核酸的单体通过从一个池到另一个池; （2）将核酸聚合物置于两个池中的一个中; 和（3）测量，因为单链核酸聚合物的每个核苷酸单体通过通道，以便在进入通道之前与另一个核苷酸单体杂交的核苷酸单体与未杂交的核苷酸单体区分 进入通道之前的另一种核苷酸单体。

公开(公告)号：US20100267026A1

公开(公告)日：2010-10-21

申请号：US12626014

申请日：2009-11-25

申请人： Mark Akeson ,  Daniel Branton ,  David W. Deamer ,  Jeffrey R. Sampson

发明人： Mark Akeson ,  Daniel Branton ,  David W. Deamer ,  Jeffrey R. Sampson

IPC分类号： C12Q1/68

CPC分类号： G01N33/48721 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2300/0896 ,  B01L2400/0475 ,  B82Y5/00 ,  B82Y30/00 ,  C12Q1/6869 ,  C12Q2565/631 ,  C12Q2521/543

摘要： Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.

摘要翻译： 提供了用于分析聚合物的系统和方法，例如多核苷酸。 该系统能够以指定的速率分析聚合物。 一个这样的分析系统包括具有纳米孔孔的结构和邻近纳米孔孔的分子马达，例如聚合酶。

公开(公告)号：US20080102504A1

公开(公告)日：2008-05-01

申请号：US11824949

申请日：2007-07-03

申请人： Mark Akeson ,  Daniel Branton ,  David Deamer ,  Jeffrey Sampson

发明人： Mark Akeson ,  Daniel Branton ,  David Deamer ,  Jeffrey Sampson

IPC分类号： C07H21/04 ,  C12N11/04 ,  C12N13/00

CPC分类号： G01N33/48721 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2300/0896 ,  B01L2400/0475 ,  B82Y5/00 ,  B82Y30/00 ,  C12Q1/6869 ,  C12Q2565/631 ,  C12Q2521/543

摘要： Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.

摘要翻译： 提供了用于分析聚合物的系统和方法，例如多核苷酸。 该系统能够以指定的速率分析聚合物。 一个这样的分析系统包括具有纳米孔孔的结构和邻近纳米孔孔的分子马达，例如聚合酶。

公开(公告)号：US20070281329A1

公开(公告)日：2007-12-06

申请号：US11717319

申请日：2007-03-13

申请人： Mark Akeson ,  Daniel Branton ,  George Church ,  David Deamer

发明人： Mark Akeson ,  Daniel Branton ,  George Church ,  David Deamer

IPC分类号： C12Q1/34 ,  C12Q1/00 ,  G01N33/00

CPC分类号： G01N33/48721 ,  B82Y15/00 ,  B82Y30/00 ,  C07K14/705 ,  C12N9/1241 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6869 ,  G01N33/68 ,  Y10S977/795 ,  Y10S977/88 ,  Y10S977/953 ,  Y10S977/957 ,  Y10S977/958 ,  C12Q2565/631 ,  C12Q2523/307 ,  C12Q2565/607

摘要： The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.

摘要翻译： 本发明涉及一种用于通过（1）在两个池之间提供两个独立的相邻的介质池和界面来检测核酸中的双链区域的方法，所述界面具有如此尺寸以允许顺序单体 单链核酸但不是双链核酸的单体通过从一个池到另一个池; （2）将核酸聚合物置于两个池中的一个中; 和（3）测量，因为单链核酸聚合物的每个核苷酸单体通过通道，以便在进入通道之前与另一个核苷酸单体杂交的核苷酸单体与未杂交的核苷酸单体区分 进入通道之前的另一种核苷酸单体。

公开(公告)号：US20070262050A1

公开(公告)日：2007-11-15

申请号：US11008402

申请日：2004-12-09

申请人： Jene Golovchenko ,  Gavin King ,  Gregor Schurmann ,  Daniel Branton

发明人： Jene Golovchenko ,  Gavin King ,  Gregor Schurmann ,  Daniel Branton

IPC分类号： C03C25/68 ,  B44C1/22

CPC分类号： C23C14/06 ,  B82Y10/00 ,  C23C14/5873 ,  H01J37/3005 ,  H01J2237/31732 ,  H01J2237/3174 ,  H01L21/02238 ,  H01L21/0332 ,  H01L21/0337 ,  H01L21/30604 ,  H01L21/3065 ,  H01L21/31662 ,  H01L21/3185 ,  Y10S977/856 ,  Y10S977/857 ,  Y10S977/888 ,  Y10S977/89 ,  Y10S977/891

摘要： The invention provides a method for forming a patterned material layer on a structure, by condensing a vapor to a solid condensate layer on a surface of the structure and then localized removal of selected regions of the condensate layer by directing a beam of energy at the selected regions. The structure can then be processed, with at least a portion of the patterned solid condensate layer on the structure surface, and then the solid condensate layer removed. Further there can be stimulated localized reaction between the solid condensate layer and the structure by directing a beam of energy at at least one selected region of the condensate layer.

摘要翻译： 本发明提供了一种用于在结构上形成图案化材料层的方法，其通过将蒸汽冷凝在结构表面上的固体冷凝物层，然后通过将所选择的能量束引导到选定的区域来局部去除冷凝物层的选定区域 地区。 然后可以将结构加工成具有结构表面上的图案化固体冷凝物层的至少一部分，然后去除固体冷凝物层。 此外，通过在冷凝物层的至少一个选定区域处引导能量束，可以刺激固体冷凝物层和结构之间的局部反应。

公开(公告)号：US07238485B2

公开(公告)日：2007-07-03

申请号：US11088140

申请日：2005-03-23

申请人： Mark Akeson ,  Daniel Branton ,  David W. Deamer ,  Jeffrey R. Sampson

发明人： Mark Akeson ,  Daniel Branton ,  David W. Deamer ,  Jeffrey R. Sampson

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： G01N33/48721 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2300/0896 ,  B01L2400/0475 ,  B82Y5/00 ,  B82Y30/00 ,  C12Q1/6869 ,  C12Q2565/631 ,  C12Q2521/543

摘要： Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.

摘要翻译： 提供了用于分析聚合物的系统和方法，例如多核苷酸。 该系统能够以指定的速率分析聚合物。 一个这样的分析系统包括具有纳米孔孔的结构和邻近纳米孔孔的分子马达，例如聚合酶。

公开(公告)号：US20050053961A1

公开(公告)日：2005-03-10

申请号：US10739585

申请日：2003-12-18

申请人： Mark Akeson ,  Daniel Branton ,  Geroge Church ,  David Deamer

发明人： Mark Akeson ,  Daniel Branton ,  Geroge Church ,  David Deamer

IPC分类号： C07K14/705 ,  C12N9/12 ,  C12Q1/68 ,  G01N27/04 ,  G01N33/68 ,  C12M1/34 ,  C12N9/00

CPC分类号： G01N33/48721 ,  B82Y15/00 ,  B82Y30/00 ,  C07K14/705 ,  C12N9/1241 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6869 ,  G01N33/68 ,  Y10S977/795 ,  Y10S977/88 ,  Y10S977/953 ,  Y10S977/957 ,  Y10S977/958 ,  C12Q2565/631 ,  C12Q2523/307 ,  C12Q2565/607

摘要： The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.

摘要翻译： 本发明涉及一种用于通过（1）在两个池之间提供两个独立的相邻的介质池和界面来检测核酸中的双链区域的方法，所述界面具有如此尺寸以允许顺序单体 单链核酸但不是双链核酸的单体通过从一个池到另一个池; （2）将核酸聚合物置于两个池中的一个中; 和（3）测量，因为单链核酸聚合物的每个核苷酸单体通过通道，以便在进入通道之前与另一个核苷酸单体杂交的核苷酸单体与未杂交的核苷酸单体区分 进入通道之前的另一种核苷酸单体。

公开(公告)号：US5795782A

公开(公告)日：1998-08-18

申请号：US405735

申请日：1995-03-17

申请人： George Church ,  David W. Deamer ,  Daniel Branton ,  Richard Baldarelli ,  John Kasianowicz

发明人： George Church ,  David W. Deamer ,  Daniel Branton ,  Richard Baldarelli ,  John Kasianowicz

IPC分类号： C07K14/705 ,  C12N9/12 ,  C12Q1/68 ,  G01N27/04 ,  G01N33/68 ,  G01N33/483

CPC分类号： G01N33/68 ,  C07K14/705 ,  C12N9/1241 ,  C12Q1/6869 ,  G01N33/48721 ,  B82Y30/00 ,  Y10S977/781 ,  Y10S977/788 ,  Y10S977/84 ,  Y10S977/88 ,  Y10S977/924 ,  Y10S977/953

摘要： A method is disclosed for characterizing a linear polymer molecule by measuring physical changes across an interface between two pools of media as the linear polymer traverses the interface and monomers of the polymer interact with the interface, where the physical changes are suitable to identify characteristics of the polymer.

摘要翻译： 公开了一种用于表征线性聚合物分子的方法，当线性聚合物穿过界面并测量聚合物的单体与界面相互作用时，通过测量两个介质池之间的界面上的物理变化，其中物理变化适合于识别 聚合物。