公开(公告)号：US07964388B2

公开(公告)日：2011-06-21

申请号：US12654717

申请日：2009-12-30

申请人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

发明人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

IPC分类号： C12M1/34

CPC分类号： G01N33/54366

摘要： A device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution. A chemical analysis device is also provided to capture molecules in the solution and subsequently measure the captured molecules. Reaction efficiency and sample throughput are thereby improved. The chemical reaction and chemical analysis devices use a microfluidic device channel as a reaction vessel. The channel is provided with a particular molecule immobilized on an interior surface of the channel with an obstacle positioned against the flow. In a typical reaction vessel having an enzyme immobilized in the capillary's interior surface and glass beads as obstacles, a reaction solution can move either in one direction or back and forth to react with the immobilized enzyme. The flow of the reaction solution is not laminar such that a reaction between the particular molecule and the reaction solution proceeds at high efficiency.

摘要翻译： 提供了用于固定在固相上的分子与溶液中的分子之间的化学反应的装置。 还提供化学分析装置以捕获溶液中的分子并随后测量捕获的分子。 从而提高反应效率和样品通过量。 化学反应和化学分析装置使用微流体装置通道作为反应容器。 该通道设置有固定在通道的内表面上的特定分子，并具有抵抗流动的位置。 在具有固定在毛细管内表面中的酶和玻璃珠作为障碍物的典型反应容器中，反应溶液可以在一个方向或来回移动以与固定化酶反应。 反应溶液的流动不是层流，使得特定分子和反应溶液之间的反应以高效率进行。

公开(公告)号：US07879290B2

公开(公告)日：2011-02-01

申请号：US12034880

申请日：2008-02-21

申请人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

发明人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

IPC分类号： G01N21/76 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N21/00 ,  G01N21/75

CPC分类号： C12Q1/04 ,  G01N21/76

摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.

摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光盒，其具有样品容器保持器和其中的快门单元，所述快门单元包括顶板，该顶板部分地通过板构件的运动和在其中具有光电检测器的第二遮光箱而形成。 虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到 通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

公开(公告)号：US20100216183A1

公开(公告)日：2010-08-26

申请号：US12714185

申请日：2010-02-26

申请人： Masahiro Okanojo ,  Hideyuki Noda ,  Noe Miyashita

发明人： Masahiro Okanojo ,  Hideyuki Noda ,  Noe Miyashita

IPC分类号： C12Q1/04 ,  C12M1/34

CPC分类号： C12Q1/04 ,  C12Q1/24 ,  C12Q1/66

摘要： The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.

摘要翻译： 样品容器具有两层膜过滤器，其包括作为亲水膜过滤器的上层的第一层和作为第二层的疏水膜过滤器，其能够不使用润湿剂并且通过手段过滤水溶液 形成负压。 使用该样品容器，通过由抽吸部分形成的负压过滤大量的水样品溶液，以通过亲水膜过滤器捕获含水样品溶液中的微生物。 然后，将负压恢复到正常压力，然后将微生物溶解溶液加入到膜过滤器中，以将微生物溶解溶液在给定时间内保留在疏水膜过滤器上。 然后，将微生物溶解溶液分配到含有发光试剂的反应容器中，检测发光以检测微生物。

公开(公告)号：US07666662B2

公开(公告)日：2010-02-23

申请号：US10854018

申请日：2004-05-26

申请人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

发明人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

IPC分类号： C12M1/34

CPC分类号： G01N33/54366

摘要： A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel. In a typical reaction vessel having an enzyme immobilized on an interior surface of a capillary and glass beads functioning as an obstacle for the flow filled in the channel of the capillary, a reaction solution can move either in one direction or back and forth in two directions to thereby undergo a reaction with the enzyme immobilized on the interior surface. The flow of the reaction solution is not a laminar flow so that a reaction between the particular molecule immobilized and the reaction solution proceeds at high efficiency.

摘要翻译： 提供化学反应装置用于固定在固相上的分子和溶液中的分子之间的化学反应，并且还提供化学分析装置，以通过化学反应和随后的固定在固相上的分子捕获溶液中的分子 捕获的分子的测量。 从而提高了反应效率以及样品通量。 化学反应装置和化学分析装置使用用于反应容器的微流体装置的通道，并且至少一个特定的分子固定在内表面上，并且固定结构或非固定的阻碍流动的障碍物 渠道。 在具有固定在毛细管的内表面上的酶的典型的反应容器和作为填充在毛细管的通道中的流动的障碍物的玻璃珠上，反应溶液可以在一个方向或两个方向上来回移动 从而与固定在内表面上的酶进行反应。 反应溶液的流动不是层流，使得固定的特定分子与反应溶液之间的反应以高效率进行。

公开(公告)号：US20080241871A1

公开(公告)日：2008-10-02

申请号：US12010497

申请日：2008-01-25

申请人： Masahiro Okanojo ,  Satoshi Ozawa ,  Hideyuki Noda ,  Masaaki Hirano

发明人： Masahiro Okanojo ,  Satoshi Ozawa ,  Hideyuki Noda ,  Masaaki Hirano

IPC分类号： C12Q1/02 ,  G01N21/76 ,  C12M1/00

CPC分类号： G01N21/11 ,  G01N21/274 ,  G01N21/6428 ,  G01N2021/7786

摘要： The present invention provides a luminescence measuring method that can be accurately and quickly carried out while inhibiting a possible background associated with viable bacteria adhering to a nozzle or Adenosine Tri Phosphate remaining in the nozzle, and an apparatus for the method. The present invention uses a washing apparatus characterized by including a nozzle, a lysys solution, a luminescence reagent solution, and a detection section, as well as a relevant washing method and a relevant luminescence measuring method. To remove viable bacteria adhering to the nozzle, the nozzle is immersed in the lysys solution and then in the luminescence reagent solution. The detection section monitors luminescence occurring during a washing process.

摘要翻译： 本发明提供一种能够准确，快速地进行发光测定的方法，同时抑制与残留在喷嘴中的喷嘴或三磷酸腺苷的活菌相关的可能背景，以及该方法的装置。 本发明使用一种洗涤装置，其特征在于包括喷嘴，溶解液，发光试剂溶液和检测部分，以及相关的洗涤方法和相关的发光测量方法。 为了除去附着在喷嘴上的活细菌，将喷嘴浸入溶解液中，然后浸入发光试剂溶液中。 检测部分监视在洗涤过程中发生的发光。

公开(公告)号：US5052766A

公开(公告)日：1991-10-01

申请号：US275600

申请日：1988-11-23

申请人： Hideyuki Noda ,  Masato Koike

发明人： Hideyuki Noda ,  Masato Koike

IPC分类号： G02B5/18 ,  G02B5/32

CPC分类号： G02B5/1857 ,  G02B5/32 ,  Y10S359/90

摘要： In formulating a groove pattern for a diffraction grating by means of holography, unique groove patterns are obtained by use of astigmatic coherent beam as either one or two recording beams. Instead of use of only stigmatic beams, and astigmatism in recording light in manufacture of a halographic grating will bring more utilizable parameters in designing groove patterns by which new loci of the type of non-hyperboloidal curves are formed on grating substrates and thereby elimination of abberations which were conventionally difficult are achieved with various optical devices.

摘要翻译： 在通过全息技术为衍射光栅制造凹槽图案时，通过使用像散相干光束作为一个或两个记录光束获得独特的凹槽图案。 代替仅使用桁架光束，并且在制造光晕光栅时记录光的散光将在设计凹槽图案中带来更多可利用的参数，通过该凹槽图案，在光栅基板上形成非双曲面曲线类型的新轨迹，从而消除湮没 传统上难以实现的各种光学装置。

公开(公告)号：US20130139898A1

公开(公告)日：2013-06-06

申请号：US13677462

申请日：2012-11-15

申请人： Noe Miyashita ,  Hideyuki Noda ,  Masahiro Okanojo

发明人： Noe Miyashita ,  Hideyuki Noda ,  Masahiro Okanojo

IPC分类号： B01L3/00 ,  F17D3/00

CPC分类号： B01L3/52 ,  F17D3/00 ,  G01N21/763 ,  G01N35/1002 ,  G01N35/1011 ,  G01N2035/102 ,  G01N2035/1032 ,  Y10T137/0324 ,  Y10T436/118339 ,  Y10T436/119163 ,  Y10T436/2575

摘要： A reagent splitting/dispensing method based on a reagent dispensing nozzle controls a split or dispensation amount of reagent, includes a waiting process of disposing a first air layer between an interface of the operation fluid and a nozzle tip end in the reagent dispensing nozzle; a first moving process of moving the reagent dispensing nozzle to a position above the reagent to be split; a second moving process of depositing the nozzle tip end in the reagent; an air layer adjusting process of increasing the occupation amount of the operation fluid in the reagent dispensing nozzle and decreasing the occupation amount of the first air layer, between the first moving process and the second moving process; and a reagent splitting process of decreasing the occupation amount of the operation fluid in the reagent dispensing nozzle and filling the reagent into the reagent dispensing nozzle from the nozzle tip end.

摘要翻译： 基于试剂分注喷嘴的试剂分注/分配方法控制分液或分配量的试剂，包括在操作流体的界面和试剂分配喷嘴中的喷嘴前端之间设置第一空气层的等待过程; 将试剂分注喷嘴移动到待分离试剂上方的位置的第一移动处理; 将所述喷嘴前端沉积在所述试剂中的第二移动过程; 在所述第一移动处理和所述第二移动处理之间增加所述试剂分注用喷嘴中的操作流体的占有量并减少所述第一空气层的占有量的空气层调整处理; 以及试剂分解处理，其减少试剂分注喷嘴中的操作流体的占有量并将试剂从喷嘴前端填充到试剂分注喷嘴中。

公开(公告)号：US20120321520A1

公开(公告)日：2012-12-20

申请号：US13580674

申请日：2010-12-22

申请人： Masahiro Okanojo ,  Hideyuki Noda

发明人： Masahiro Okanojo ,  Hideyuki Noda

IPC分类号： G01N1/14 ,  G01N21/64

CPC分类号： G01N35/1011 ,  G01N21/6428

摘要： The present invention enables liquid suction with a nozzle tip end in contact with a bottom surface of a container without causing damage to the nozzle and/or the bottom surface of the container.Plural rails 112 are disposed on an arm 101 movable three-dimensionally so as to extend upward from the arm 101, and a pipe 102 is fixed to a pipe fixing member 103 movable along the rails. A tip end of the pipe functions as a nozzle. Plural resilient members 114 are disposed on the arm so as to correct the inclination of the pipe fixing member. When the arm is moved downward and the tip end of the pipe comes in contact with the bottom of the container 106, the pipe and the pipe fixing portion move upward along the rails, so that the tip end of the pipe can be brought into contact with the bottom surface of the container while avoiding damages in the pipe and bottom of the container and suck liquid in the container in this state.

摘要翻译： 本发明能够使喷嘴前端的液体吸附与容器的底面接触而不会损坏容器的喷嘴和/或底面。 多个轨道112设置在能够从臂101上向三维移动的臂101上，并且管102固定到沿着轨道移动的管固定构件103。 管的尖端用作喷嘴。 多个弹性构件114设置在臂上，以便校正管固定构件的倾斜度。 当臂向下移动并且管的顶端与容器106的底部接触时，管和管固定部沿着轨道向上移动，使得管的顶端可以接触 同时避免容器的管道和底部的损坏，并且在该状态下吸入容器中的液体。

公开(公告)号：US08334144B2

公开(公告)日：2012-12-18

申请号：US13478527

申请日：2012-05-23

申请人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

发明人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

IPC分类号： G01N21/76 ,  G01N21/00 ,  G01N21/75

CPC分类号： C12Q1/04 ,  G01N21/76

摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.

摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光箱，其具有样本容器保持器和其中的快门单元，所述快门单元包括部分地由板构件的运动形成的顶板和在其中具有光电检测器的第二遮光箱。 虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到 通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

公开(公告)号：US20120312083A1

公开(公告)日：2012-12-13

申请号：US13579329

申请日：2011-02-23

申请人： Rena Akahori ,  Takashi Anazawa ,  Satoshi Ozawa ,  Yoshiaki Yazawa ,  Tomoyuki Sakai ,  Hideyuki Noda

发明人： Rena Akahori ,  Takashi Anazawa ,  Satoshi Ozawa ,  Yoshiaki Yazawa ,  Tomoyuki Sakai ,  Hideyuki Noda

IPC分类号： G01N33/48

CPC分类号： C12Q1/6869 ,  G01N21/6428 ,  G01N33/48721 ,  C12Q2565/631

摘要： There have been the following problems with sequence analysis using multiple nanopores: trapping a sample in the nanopores is not always 100% efficient and unnecessary time is spent to measure pores in which no sample has been trapped, resulting in low measurement efficiency. To address the problems, a labeling substance is boned to a sample, and the sample to which the labeling substance has been bonded is trapped in the nanopores. An apparatus for observing the labeling substance is used to observe the labeling substance and monitor whether or not the sample has been trapped in the nanopores. Measuring only nanopores in which the sample has been trapped allows the measurement efficiency to be improved.

摘要翻译： 使用多个纳米孔的序列分析存在以下问题：在纳米孔中捕获样品并不总是100％的有效性，并且花费不需要的时间来测量没有样品被捕获的孔隙，导致低的测量效率。 为了解决这些问题，将标记物质结合到样品中，并且标记物质已经结合的样品被捕获在纳米孔中。 用于观察标记物质的装置用于观察标记物质并监测样品是否已被捕获在纳米孔中。 仅测量样品已被捕获的纳米孔，可以提高测量效率。