利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

57 条记录 (耗时 0.054 秒)

    Methods and kit for hybridization anaylsis using peptide nucleic acid probes
    51.
    发明申请
    Methods and kit for hybridization anaylsis using peptide nucleic acid probes 审中-公开
    使用肽核酸探针进行杂交分析的方法和试剂盒

    公开(公告)号：US20050053944A1

    公开(公告)日：2005-03-10

    申请号：US10636343

    申请日：2003-08-07

    申请人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

    发明人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

    IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6813 ,  G01N27/447 ,  G01N33/566 ,  C07K14/47

    CPC分类号： G01N27/44726 ,  C12Q1/6813 ,  G01N27/447 ,  C12Q2525/113 ,  C12Q2523/113 ,  C12Q2565/125 ,  C12Q2565/629 ,  C12Q2565/107

    摘要： A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.

    摘要翻译： 一种方法组合物和装置，用于通过使单链核酸样品与具有与靶序列互补的序列的可检测PNA探针接触来杂交和分离样品中具有所需靶序列的分子，使得靶序列（如果存在） 将与可检测的探针杂交以形成可检测的双链体，然后通过电泳将变性培养基中的双链体与未结合的样品组分分离。 本发明还涉及用于通过使样品与可检测的PNA探针接触来在单链核酸及其互补链的混合样品中杂交和分离具有所需靶序列的分子的方法组合物和装置。

    Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
    54.
    发明授权
    Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility 失效
    肽核酸

    公开(公告)号：US06710164B1

    公开(公告)日：2004-03-23

    申请号：US09230088

    申请日：1999-03-10

    申请人： Peter E. Nielsen ,  Michael Egholm ,  Rolf H. Berg ,  Ole Buchardt

    发明人： Peter E. Nielsen ,  Michael Egholm ,  Rolf H. Berg ,  Ole Buchardt

    IPC分类号： A61K3800

    CPC分类号： C07K5/06139 ,  C07K14/003 ,  C12Q1/68

    摘要： A novel class of compounds known as peptide nucleic acids, bind complementary DNA and RNA strands, and generally do so more strongly than the corresponding DNA or RNA strands while exhibiting increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases, including 2,6-diaminopurine, attached to a polyamide backbone, and contain alkyl amine side chains.

    摘要翻译： 被称为肽核酸的新一类化合物结合互补DNA和RNA链，并且通常比相应的DNA或RNA链更强烈地显示出增加的序列特异性和溶解性。 肽核酸包含选自天然存在的核碱基和非天然存在的核碱基的配体，包括连接到聚酰胺主链上的2,6-二氨基嘌呤，并且含有烷基胺侧链。