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    Aza-benzazolium containing cyanine dyes
    51.
    发明授权
    Aza-benzazolium containing cyanine dyes 有权
    含有氰基染料的氮杂 -

    公开(公告)号：US07871773B2

    公开(公告)日：2011-01-18

    申请号：US11756765

    申请日：2007-06-01

    申请人： Richard Haugland ,  Stephen Yue

    发明人： Richard Haugland ,  Stephen Yue

    IPC分类号： C12Q1/68 ,  C12Q1/00 ,  C12Q1/02 ,  G01N33/53 ,  C07D215/00 ,  C07D417/00 ,  C12P19/34

    CPC分类号： C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

    摘要： Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

    摘要翻译： 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

    DETECTION OF IMMOBILIZED NUCLEIC ACID
    52.
    发明申请
    DETECTION OF IMMOBILIZED NUCLEIC ACID 审中-公开
    检测固定化核酸

    公开(公告)号：US20100227331A1

    公开(公告)日：2010-09-09

    申请号：US12778774

    申请日：2010-05-12

    申请人： Matthew Paul BEAUDET ,  W. Gregory Cox ,  Stephen Yue

    发明人： Matthew Paul BEAUDET ,  W. Gregory Cox ,  Stephen Yue

    IPC分类号： C12Q1/68 ,  C07D401/06

    CPC分类号： G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

    摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

    摘要翻译： 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。 通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。 优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。 使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

    Detection of immobilized nucleic acid
    53.
    发明授权
    Detection of immobilized nucleic acid 有权
    检测固定化核酸

    公开(公告)号：US07727716B2

    公开(公告)日：2010-06-01

    申请号：US10956868

    申请日：2004-09-30

    申请人： Matthew Paul Beaudet ,  W. Gregory Cox ,  Stephen Yue

    发明人： Matthew Paul Beaudet ,  W. Gregory Cox ,  Stephen Yue

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/02 ,  C07D209/04

    CPC分类号： G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

    摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

    摘要翻译： 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。 通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。 优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。 使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

    Apparatus and methods for self-biasing differential signaling circuitry having multimode output configurations for low voltage applications
    58.
    发明授权
    Apparatus and methods for self-biasing differential signaling circuitry having multimode output configurations for low voltage applications 有权
    具有用于低电压应用的多模输出配置的用于自偏压差动信号电路的装置和方法

    公开(公告)号：US07495477B2

    公开(公告)日：2009-02-24

    申请号：US11830897

    申请日：2007-07-31

    申请人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard W. Fung

    发明人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard W. Fung

    IPC分类号： H03K19/094

    CPC分类号： H03K17/302 ,  H03K17/04106

    摘要： The present disclosure relates to a differential signaling circuit including differential signaling circuitry having at least one output and one input, that can operate in multiple mode of operations while using a single, low voltage supply source. Two or more switches are included and configured to selectively couple a supply voltage to the output dependent on a mode of operation of the differential signaling circuitry. The circuit also includes a switch control biasing circuit operatively coupled to at least one of the switches and to the output of the differential signaling circuitry. The switch control biasing circuit provides a switch control biasing voltage to control a state of the switch based on a voltage level of the output. Further, a bulk biasing circuit is included and operatively coupled to the switch. The bulk biasing circuit selectively provides a bulk biasing voltage to the switch based on the voltage level of the output.

    摘要翻译： 本公开涉及一种差分信号电路，其包括具有至少一个输出和一个输入的差分信号电路，其可以在使用单个低电压电源时在多种操作模式下操作。 两个或更多个开关被包括并配置成根据差分信号电路的操作模式选择性地将电源电压耦合到输出。 电路还包括可操作地耦合到开关中的至少一个和差分信号电路的输出的开关控制偏置电路。 开关控制偏置电路提供开关控制偏置电压，以基于输出的电压电平来控制开关的状态。 此外，体积偏置电路被包括并且可操作地耦合到开关。 体积偏置电路基于输出的电压电平选择性地向开关提供体积偏置电压。

    Aza-Benzazolium Containing Cyanine Dyes
    60.
    发明申请
    Aza-Benzazolium Containing Cyanine Dyes 有权
    含有氰基染料的氮杂 - 唑烷鎓

    公开(公告)号：US20080044811A1

    公开(公告)日：2008-02-21

    申请号：US11756765

    申请日：2007-06-01

    申请人： Richard Haugland ,  Stephen Yue

    发明人： Richard Haugland ,  Stephen Yue

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  G01N33/00 ,  G01N33/68

    CPC分类号： C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

    摘要： Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

    摘要翻译： 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。