Endonuclease-substrate complexes
    54.
    发明授权
    Endonuclease-substrate complexes 有权
    内切核酸酶 - 底物复合物

    公开(公告)号:US07482118B2

    公开(公告)日:2009-01-27

    申请号:US10783557

    申请日:2004-02-20

    IPC分类号: C12Q1/68

    CPC分类号: C12Q1/6823 C12Q2521/301

    摘要: The present invention provides novel cleavage agents for the cleavage and modification of nucleic acid. The cleavage agents find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, an endonuclease activity is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

    摘要翻译: 本发明提供了用于切割和修饰核酸的新的切割剂。 裂解剂可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中,使用内切核酸酶活性来切割靶依赖性切割结构,从而指示特异性核酸序列的存在或其特定变体。

    Detection of nucleic acids
    55.
    发明申请
    Detection of nucleic acids 有权
    检测核酸

    公开(公告)号:US20080131890A1

    公开(公告)日:2008-06-05

    申请号:US11809567

    申请日:2007-06-01

    IPC分类号: C12Q1/68

    摘要: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.

    摘要翻译: 本发明涉及用于检测和表征小核酸分子(例如RNA(例如,小RNA如微RNA(miRNA)和小干扰RNA(siRNA))和其它短核酸分子的组合物和方法。 更具体地说,本发明涉及检测和定量RNA表达的方法。 本发明进一步提供了miRNA和siRNA变体的检测。

    Methods and applications for target quantification
    59.
    发明授权
    Methods and applications for target quantification 有权
    目标定量的方法和应用

    公开(公告)号:US08190371B2

    公开(公告)日:2012-05-29

    申请号:US12206436

    申请日:2008-09-08

    IPC分类号: G06F19/00 G06F17/40

    摘要: The present invention provides methods and software applications for quantifying a target in an experimental sample by collecting and processing initial signal data from the experimental sample and at least two standard control samples containing known target copy numbers. In this regards, the present invention allows the quantification of target copy number in the experimental sample.

    摘要翻译: 本发明提供了通过收集和处理来自实验样品的初始信号数据和至少两个包含已知目标拷贝数的标准对照样品来量化实验样品中靶标的方法和软件应用。 在这方面,本发明允许定量实验样品中的目标拷贝数。

    METHODS AND APPLICATIONS FOR TARGET QUANTIFICATION
    60.
    发明申请
    METHODS AND APPLICATIONS FOR TARGET QUANTIFICATION 有权
    目标定量的方法和应用

    公开(公告)号:US20090299641A1

    公开(公告)日:2009-12-03

    申请号:US12206436

    申请日:2008-09-08

    IPC分类号: G06F19/00

    摘要: The present invention provides methods and software applications for quantifying a target in an experimental sample by collecting and processing initial signal data from the experimental sample and at least two standard control samples containing known target copy numbers. In this regards, the present invention allows the quantification of target copy number in the experimental sample.

    摘要翻译: 本发明提供了通过收集和处理来自实验样品的初始信号数据和至少两个包含已知目标拷贝数的标准对照样品来量化实验样品中靶标的方法和软件应用。 在这方面,本发明允许定量实验样品中的目标拷贝数。