中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

70 records (took 0.026 seconds)

    COMPOSITIONS AND METHODS FOR NUCLEIC ACID ISOLATION
    53.
    发明公开
    COMPOSITIONS AND METHODS FOR NUCLEIC ACID ISOLATION 审中-公开

    公开(公告)号：US20230407288A1

    公开(公告)日：2023-12-21

    申请号：US18252283

    申请日：2021-11-10

    Applicant: LIFE TECHNOLOGIES AS

    Inventor： Laure JOBERT ,  Marie BOSNES ,  Hannah Kim LINDSTROM

    IPC: C12N15/10 ,  C12P19/34

    CPC classification number: C12N15/1013 ,  C12P19/34

    Abstract: A method of separating RNA from a sample, comprising providing: a sample comprising RNA, a binding solution comprising an oligoethylene glycol and a salt, and a solid support having a hydrophilic surface. The method further comprises contacting the sample with the binding solution and solid support, under conditions that allow binding of the RNA in the sample to the surface of the solid support, thereby providing a solid support with bound RNA in contact with residual solution; and separating the solid support with bound RNA from the residual solution. During the binding of the RNA to the surface of the solid support, oligoethylene glycol is present at a concentration of at least about 35% v/v and the salt is present at a concentration of between about 1 M and about 2 M. Methods for producing purified RNA are also provided, comprising in vitro transcription to produce an RNA molecule with a first magnetic bead and purification with a second magnetic bead. Also provided are kits and uses.

    Methods and compositions for nucleic acid isolation
    60.
    发明授权
    Methods and compositions for nucleic acid isolation 有权

    公开(公告)号：US10787659B2

    公开(公告)日：2020-09-29

    申请号：US16137448

    申请日：2018-09-20

    Applicant: LIFE TECHNOLOGIES AS

    Inventor： Darren Ellis ,  Hannah Lindstroem

    IPC: C12N15/10

    Abstract: The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves mixing dibromochloromethane, iodochloromethane or mixtures thereof with a biological sample that has been mixed with a phenol-based extraction solution. Centrifugation of the sample is not needed to achieve phase separation which occurs using the present methods in as little as one to two minutes with no phenol carryover.

Patent Agency Ranking