公开(公告)号：US06391567B1

公开(公告)日：2002-05-21

申请号：US09517605

申请日：2000-03-02

申请人： Dan R. Littman ,  Douglas Kwon ,  Yvette Van Kooyk ,  Teunis Geijtenbeek

发明人： Dan R. Littman ,  Douglas Kwon ,  Yvette Van Kooyk ,  Teunis Geijtenbeek

IPC分类号： G01N3353

CPC分类号： C07K16/1063 ,  A01K2217/05 ,  A61K38/178 ,  A61K39/00 ,  A61K2039/505 ,  A61K2039/525 ,  C07K14/70525 ,  C07K2317/77 ,  G01N33/5032 ,  G01N33/505 ,  G01N2333/162

摘要： The present invention demonstrates that a receptor, named DC-SIGN, is specifically expressed on dendritic cells, and facilitates infection of T lymphocytes with HIV. Assays for identifying compounds that modulate the interaction of DC-SIGN and HIV and/or T cells and macrophage are provided. Compounds so identified are also part of the present invention.

摘要翻译： 本发明表明，一种称为DC-SIGN的受体在树突状细胞上特异性表达，并且有助于HIV感染T淋巴细胞。 提供了用于鉴定调节DC-SIGN和HIV和/或T细胞和巨噬细胞的相互作用的化合物的测定。 如此鉴定的化合物也是本发明的一部分。

公开(公告)号：US06344545B1

公开(公告)日：2002-02-05

申请号：US08831823

申请日：1997-04-02

申请人： Graham P. Allaway ,  Virginia M. Litwin ,  Paul J. Maddon ,  William C. Olson

发明人： Graham P. Allaway ,  Virginia M. Litwin ,  Paul J. Maddon ,  William C. Olson

IPC分类号： C07K1628

CPC分类号： C07K16/2866 ,  A61K2039/505 ,  C07K16/24 ,  C07K2317/76 ,  G01N33/566 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2333/70514 ,  G01N2333/715 ,  G01N2500/20

摘要： This invention provides methods for inhibiting fusion of HIV-1 to CD4+ cells which comprise contacting CD4+ cells with a non-chemokine agent capable of binding to a chemokine receptor in an amount and under conditions such that fusion of HIV-1 to the CD4+ cells is inhibited. This invention also provides methods for inhibiting HIV-1 infection of CD4+ cells which comprise contacting CD4+ cells with a non-chemokine agent capable of binding to a chemokine receptor in an amount and under conditions such that fusion of HIV-1 to the CD4+ cells is inhibited, thereby inhibiting the HIV-1 infection. This invention provides non-chemokine agents capable of binding to the chemokine receptor and inhibiting fusion of HIV-1 to CD4+ cells. This invention also provides pharmaceutical compositions comprising an amount of the non-chemokine agent capable of binding to the chemokine receptor and inhibiting fusion of HIV-1 to CD4+ cells effective to prevent fusion of HIV-1 to CD4+ cells and a pharmaceutically acceptable carrier.

公开(公告)号：US20010039026A1

公开(公告)日：2001-11-08

申请号：US09759841

申请日：2001-01-12

发明人： Susan Dobbs ,  Manoussos Perros ,  Graham Anthony Rickett

IPC分类号： G01N033/537 ,  C12Q001/70

CPC分类号： G01N33/56988 ,  G01N33/566 ,  G01N2333/162 ,  G01N2333/705

摘要： An assay method for determining whether an agent is capable of modulating the interaction of CCR5 and gp120 is disclosed. The method comprises incubating the agent with CCR5 and gp120 to form a first reaction mixture, and determining whether said agent modulates the interaction of CCR5 with gp120, wherein said gp120 is associated with CD4. In particular, the interaction is a low affinity binding.

摘要翻译： 公开了一种用于确定试剂是否能够调节CCR5和gp120的相互作用的测定方法。 该方法包括将该试剂与CCR5和gp120孵育以形成第一反应混合物，并确定所述试剂是否调节CCR5与gp120的相互作用，其中所述gp120与CD4相关。 特别地，相互作用是低亲和力结合。

公开(公告)号：US06203974B1

公开(公告)日：2001-03-20

申请号：US09146093

申请日：1998-09-03

申请人： Dinesh O. Shah ,  James P. Mackowiak ,  Natalie Dubovoy

发明人： Dinesh O. Shah ,  James P. Mackowiak ,  Natalie Dubovoy

IPC分类号： C12Q170

CPC分类号： G01N33/582 ,  G01N2333/15 ,  G01N2333/161 ,  G01N2333/162 ,  G01N2333/44 ,  Y10S435/968 ,  Y10S435/969 ,  Y10S435/971 ,  Y10S435/974 ,  Y10S435/975

摘要： The subject invention relates generally to immunoassays for detection of antibodies by use chemiluminescent compounds. More particularly, the subject invention relates to chemiluminescent immunoassays to detect antibodies wherein a precomplex mixture is created and a two-step assay is performed resulting in a greater signal.

摘要翻译： 本发明一般涉及通过使用化学发光化合物检测抗体的免疫测定。 更具体地，本发明涉及用于检测抗体的化学发光免疫测定，其中产生预复合物混合物并进行两步测定，导致更大的信号。

公开(公告)号：US6149910A

公开(公告)日：2000-11-21

申请号：US433428

申请日：1999-11-04

申请人： Robert De Leys ,  Jian Zheng

发明人： Robert De Leys ,  Jian Zheng

IPC分类号： C07K14/16 ,  G01N33/569 ,  A61K39/21 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/38

CPC分类号： C07K14/005 ,  G01N33/56988 ,  C07K2319/00 ,  C12N2740/16122 ,  G01N2333/162

摘要： This invention relates to peptides and their preparation. The peptides each have a sequence that corresponds to the immunodominant region of the HIV-1 group O gp41 envelope protein. The sequence is characterized in that it does not correspond to any known naturally occurring group O sequence or variant. Furthermore, the peptide binds anti-HIV-1 group O antibodies. There are several uses for the peptides, including the detection of antibodies produced in response to HIV-1 group O infection. The peptides may also be incorporated in mosaics and expressed recombinantly.

摘要翻译： 本发明涉及肽及其制备方法。 肽各自具有对应于HIV-1组O gp41包膜蛋白的免疫显性区域的序列。 该序列的特征在于其不对应于任何已知的天然存在的基团O序列或变体。 此外，肽结合抗HIV-1组O抗体。 肽的几种用途，包括检测响应于HIV-1组O感染产生的抗体。 肽也可以掺入马赛克并重组表达。

公开(公告)号：US5976785A

公开(公告)日：1999-11-02

申请号：US811150

申请日：1991-12-20

申请人： Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

发明人： Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

IPC分类号： A61K38/00 ,  A61K39/00 ,  A61P31/18 ,  C07K7/06 ,  C07K14/16 ,  C12N7/00 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C07K7/06 ,  C07K14/005 ,  C12N7/00 ,  C12Q1/703 ,  G01N33/56988 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  G01N2333/162 ,  G01N2469/20

摘要： A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In one embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.

摘要翻译： 公开了用于诊断HIV-2（LAV-II）感染的方法和用于其的试剂盒。 这些试剂包括能够与HIV-2的至少一部分基因组杂交的cDNA探针。 在一个实施方案中，DNA探针能够与HIV-2的整个基因组杂交。 这些试剂还包括由这些DNA序列中的一些编码的多肽。

公开(公告)号：US5955377A

公开(公告)日：1999-09-21

申请号：US403565

申请日：1995-04-17

申请人： Diana M. Maul ,  Gregory R. Bogart

发明人： Diana M. Maul ,  Gregory R. Bogart

IPC分类号： G01N33/543 ,  G01N33/547

CPC分类号： G01N33/543 ,  G01N33/547 ,  G01N2333/135 ,  G01N2333/162 ,  G01N2333/22 ,  G01N2333/295 ,  G01N2333/315 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S436/805

摘要： Method for detecting an analyte of interest, comprising the steps of providing a detection device comprising a light reflective or transmissive substrate supporting one or more layers comprising an adhering attachment layer to which is affixed a receptive material which specifically interacts with the analyte of interest; reacting the device with a sample potentially comprising the analyte under conditions in which the analyte binds to the receptive material; and reacting bound analyte with a reagent which creates a mass change on the surface of the device.

摘要翻译： 用于检测感兴趣分析物的方法，包括以下步骤：提供检测装置，所述检测装置包括支撑一层或多层的光反射或透射基底，所述层包含粘附的附着层，所述粘附附着层与感兴趣的物质特异性相互作用的受体材料固定在其上; 在所述分析物与所述受体材料结合的条件下使所述装置与潜在地包含所述分析物的样品反应; 并使结合的分析物与在装置的表面上产生质量变化的试剂反应。

公开(公告)号：US5889158A

公开(公告)日：1999-03-30

申请号：US466704

申请日：1995-06-06

申请人： Luc Montagnier ,  Denise Guetard ,  Francoise Brun-Vezinet ,  Francois Clavel

发明人： Luc Montagnier ,  Denise Guetard ,  Francoise Brun-Vezinet ,  Francois Clavel

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/16 ,  C12N7/00 ,  G01N33/569 ,  C07K16/00

CPC分类号： C07K14/005 ,  C12N7/00 ,  G01N33/56988 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/16021 ,  C12N2740/16022 ,  C12N2740/16122 ,  C12N2740/16222 ,  G01N2333/162 ,  G01N2469/20 ,  Y10S435/81 ,  Y10S435/974 ,  Y10S530/826

摘要： This invention discloses the identification and characterization of a novel human retrovirus, originally designated lymphadenopathy-associated virus type II, or LAV-II, and subsequently redesignated the human immunodeficiency virus type 2, or HIV-2. This virus was isolated from West African AIDS patients and propagated on immortalized lymphocytic cell lines or donor peripheral blood mononuclear cells (PBMCs). Immunological and nucleic acid hybridization studies demonstrated that HIV-2 differs significantly from HIV-1, the aetiological agent of AIDS. Additional biochemical characterization identified viral antigens having molecular weights of 16, 26, 36, and 130-140 kDa, as determined by SDS-PAGE. These proteins were subsequently designated p16, p26, gp36, and gp130-140, respectively. These antigens can be employed, inter alia, in the generation of both polyclonal and monoclonal antibodies, which should prove useful in diagnostic and viral antigen purification applications.

摘要翻译： 本发明公开了一种新型人类逆转录病毒（最初称为II型淋巴腺病毒或LAV-II型）的鉴定和鉴定，随后重新指定了人类免疫缺陷病毒2型或HIV-2型。 该病毒从西非艾滋病患者中分离出来，并在永生化淋巴细胞系或供体外周血单个核细胞（PBMCs）上繁殖。 免疫和核酸杂交研究表明，HIV-2与艾滋病病原体HIV-1显着不同。 额外的生化表征鉴定了通过SDS-PAGE测定的分子量为16,26,36和130-140kDa的病毒抗原。 这些蛋白质随后分别称为p16，p26，gp36和gp130-140。 这些抗原尤其可用于产生多克隆抗体和单克隆抗体，其应证明在诊断和病毒抗原纯化应用中是有用的。

公开(公告)号：US5830641A

公开(公告)日：1998-11-03

申请号：US214299

申请日：1994-03-17

申请人： Luc Montagnier ,  Denise Guetard ,  Marc Alizon ,  Fran.cedilla.ois Clavel ,  Mireille Guyader ,  Pierre Sonigo

发明人： Luc Montagnier ,  Denise Guetard ,  Marc Alizon ,  Fran.cedilla.ois Clavel ,  Mireille Guyader ,  Pierre Sonigo

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K7/06 ,  C07K14/16 ,  C12N7/00 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C07K7/06 ,  C07K14/005 ,  C12N7/00 ,  C12Q1/703 ,  G01N33/56988 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/16122 ,  C12N2740/16222 ,  G01N2333/162 ,  G01N2469/20

摘要： The invention relates to a new class of retroviruses, designated by HIV-2, of which samples have been deposited to the ECACC under numbers 87.01.1001 and 87.01.1002 and to the NCIB under numbers 12.398 and 12.399. It relates also to antigens capable to be obtained from this virus, particularly proteins p12, p16, p26 and gp140. These various antigens can be used for the diagnosis of the disease, especially by contacting these antigens with a serum of a patient submitted to the diagnosis. It relates to immunogenic compositions containing more particularly the glycoprotein gp140. Finally it concerns nucleotidic sequences, which can be used especially as hybridization probes, derived from the RNA of HIV-2.

摘要翻译： 本发明涉及由HIV-2指定的一类新的逆转录病毒，其中样品已经按照号码87.01.1001和87.01.1002保藏在ECACC，编号12.398和12.399的NCIB。 它还涉及能够从该病毒获得的抗原，特别是蛋白质p12，p16，p26和gp140。 这些各种抗原可以用于疾病的诊断，特别是通过将这些抗原与患者的血清接触进行诊断。 本发明涉及更具体地包含糖蛋白gp140的免疫原性组合物。 最后，它涉及核苷酸序列，其可以特别用作衍生自HIV-2的RNA的杂交探针。

公开(公告)号：US5721095A

公开(公告)日：1998-02-24

申请号：US486837

申请日：1995-06-07

申请人： Lily Chan ,  Yoke Wah Sum ,  May Fong Yin ,  Lee Fang Lim

发明人： Lily Chan ,  Yoke Wah Sum ,  May Fong Yin ,  Lee Fang Lim

IPC分类号： C07K14/16 ,  C07K16/42 ,  G01N33/569 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/544 ,  G01N33/564

CPC分类号： C07K14/005 ,  C07K16/4283 ,  G01N33/56988 ,  C12N2740/14022 ,  C12N2740/16122 ,  G01N2333/162 ,  Y10S435/97 ,  Y10S435/974

摘要： A method for producing an improved solid phase antigenic reagent useful in an immunoassay for detecting antibodies specific for a virus, such as the human immunodeficiency virus, is disclosed which comprising the addition to a natural viral lysate a synthetic or recombinant viral protein or peptide. Also provided is an improved immunoassay utilizing the solid phase antigenic reagent.

摘要翻译： 公开了一种生产用于免疫测定的改进的固相抗原试剂的方法，用于检测对病毒特异性的抗体，例如人类免疫缺陷病毒，其包括加入天然病毒裂解物合成或重组病毒蛋白或肽。 还提供了利用固相抗原试剂的改进的免疫测定法。