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    MYELOPEROXIDASE, A RISK INDICATOR FOR CARDIOVASCULAR DISEASE
    51.
    发明申请
    MYELOPEROXIDASE, A RISK INDICATOR FOR CARDIOVASCULAR DISEASE 审中-公开

    公开(公告)号：US20160238603A1

    公开(公告)日：2016-08-18

    申请号：US15135730

    申请日：2016-04-22

    申请人： THE CLEVELAND CLINIC FOUNDATION

    发明人： Stanley L. Hazen ,  Renliang Zhang

    IPC分类号： G01N33/573 ,  C12Q1/28

    CPC分类号： G01N33/573 ,  A61K31/616 ,  C12N1/28 ,  C12Q1/28 ,  C12Y111/01007 ,  G01N2333/908 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/50 ,  G01N2800/52 ,  G06F19/00 ,  G06Q50/22 ,  G16H50/30 ,  Y02A90/22 ,  Y02A90/26

    摘要： Diagnostic tests for characterizing an individual's risk of developing or having a cardiovascular disease. In one embodiment the present diagnostic test comprises determining the level of myeloperoxidase (MPO) activity in a bodily sample obtained from the individual or test subject. In another embodiment, the diagnostic test comprises determining the level of MPO mass in a bodily sample obtained from the test subject. In another embodiment, the diagnostic test comprises determining the level of one or more select MPO-generated oxidation products in a bodily sample obtained from the test subject. The select MPO-generated oxidation products are dityrosine, nitrotyrosine, methionine sulphoxide or an MPO-generated lipid peroxidation products. Levels of MPO activity, MPO mass, or the select MPO-generated oxidation product in bodily samples from the test subject are then compared to a predetermined value that is derived from measurements of MPO activity, MPO mass, or the select MPO-generated oxidation product in comparable bodily samples obtained from the general population or a select population of human subjects. Such comparison characterizes the test subject's risk of developing CVD.

    MICROFLUIDIC COLLABORATIVE ENZYME ENHANCED REACTIVE CEER IMMUNOASSAY
    52.
    发明申请
    MICROFLUIDIC COLLABORATIVE ENZYME ENHANCED REACTIVE CEER IMMUNOASSAY 审中-公开
    微流感合成酶增强反应性CEER免疫

    公开(公告)号：US20160131643A1

    公开(公告)日：2016-05-12

    申请号：US14988643

    申请日：2016-01-05

    申请人： NESTEC S.A.

    发明人： India Severin ,  Katya Magonova

    IPC分类号： G01N33/543 ,  G01N33/564 ,  G01N33/552

    CPC分类号： G01N33/53 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/54306 ,  G01N2333/904 ,  G01N2333/908

    摘要： The present invention provides methods for diagnosing a disease state in a patient by detecting the presence, expression level and/or activation level of a target analyte in a patient sample using a proximity dual detection assay on a microcarrier. The present invention also provides an assay device for performing the methods described herein.

    摘要翻译： 本发明提供了通过使用微载体上的邻近双重检测测定来检测患者样品中目标分析物的存在，表达水平和/或活化水平来诊断患者疾病状态的方法。 本发明还提供了用于执行本文所述方法的测定装置。

    BIOLOGICAL FUNCTIONALISATION OF SUBSTRATES
    53.
    发明申请
    BIOLOGICAL FUNCTIONALISATION OF SUBSTRATES 审中-公开
    基材的生物功能化

    公开(公告)号：US20160022869A1

    公开(公告)日：2016-01-28

    申请号：US14872188

    申请日：2015-10-01

    申请人： THE UNIVERSITY OF SYDNEY

    发明人： Marcela Bilek ,  David McKenzie ,  Yongbai Yin

    IPC分类号： A61L27/34 ,  G01N33/543 ,  A61L27/04

    CPC分类号： A61L27/34 ,  A61L27/042 ,  A61L27/54 ,  A61L2300/252 ,  A61L2300/254 ,  A61L2300/606 ,  A61L2400/18 ,  B05D1/62 ,  G01N33/54306 ,  G01N2333/908 ,  Y10T442/30

    摘要： The invention relates to an activated metallic, semiconductor, polymer, composite and/or ceramic substrate, the substrate being bound through a mixed or graded interface to a hydrophilic polymer surface that is activated to enable direct covalent binding to a functional biological molecule, the polymer surface comprising a sub-surface that includes a plurality of cross-linked regions, as well as to such activated substrates that have been functionalised with a biological molecule and to devices comprising such functionalised substrates. Such substrates can be produced by a method comprising steps of: a. exposing a surface of the substrate to any or more of (i) to (iii): (i) plasma ion implantation with carbon containing species; (ii) co-deposition under conditions in which substrate material is deposited with carbon containing species while gradually reducing substrate material proportion and increasing carbon containing species proportion; (iii) deposition of a plasma polymer surface layer with energetic ion bombardment; incubating the surface treated according to step (a) with a desired biological molecule.

    摘要翻译： 本发明涉及活化的金属，半导体，聚合物，复合材料和/或陶瓷基材，所述基材通过混合或分级界面结合到亲水性聚合物表面，所述界面被活化以实现与功能性生物分子的直接共价结合，所述聚合物 表面，其包括包含多个交联区域的子表面，以及已经用生物分子官能化的这种活化的底物和包含这种官能化底物的装置。 这样的基材可以通过包括以下步骤的方法制备：a。 （i）至（iii）中的任何一种或多种：（i）用含碳物质进行等离子体离子注入; （ii）在使含有碳的物质沉积衬底材料的条件下共沉积，同时逐渐降低衬底材料比例并增加含碳物质比例; （iii）用能量离子轰击沉积等离子体聚合物表面层; 将根据步骤（a）处理的表面与所需生物分子一起孵育。

    Assay device comprising bubble-forming means
    54.
    发明授权
    Assay device comprising bubble-forming means 有权
    包含气泡形成装置的测定装置

    公开(公告)号：US09151749B2

    公开(公告)日：2015-10-06

    申请号：US12937465

    申请日：2009-04-09

    申请人： Balbir Raj

    发明人： Balbir Raj ,  David Tolley

    IPC分类号： C12Q1/00 ,  G01N33/543 ,  B01L3/00 ,  G01N33/58

    CPC分类号： G01N33/54366 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/0621 ,  B01L2300/025 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/046 ,  B01L2400/0622 ,  G01N33/581 ,  G01N2333/59 ,  G01N2333/908

    摘要： Disclosed is a method for determining the presence and/or amount Oran analyte of interest in a liquid sample comprising the steps of: contacting the liquid sample suspected of containing analyte with a gas generating means (eg. catalase and peracid or peroxygen compound), which gas generating means forms a gas dependent upon the presence, absence or amount of analyte, which gas creates one or more bubbles in the liquid sample which act to alter the flow of liquid along a flow path; and determining an alteration of flow in the liquid wherein the alteration of flow of liquid along the flow path is indicative of the presence and/or amount of analyte in the liquid sample.

    摘要翻译： 公开了一种用于确定液体样品中目标的存在和/或量Oran分析物的方法，包括以下步骤：将怀疑含有分析物的液体样品与气体产生装置（例如过氧化氢酶和过酸或过氧化合物）接触， 气体发生装置根据分析物的存在，不存在或数量形成气体，该气体在液体样品中产生一个或多个气泡，其用于改变液体沿着流动路径的流动; 以及确定液体中的流动的改变，其中沿着流动路径的液体流动的改变指示液体样品中分析物的存在和/或量。

    Combined F2-isoprostane and myeloperoxidase detection, a risk indicator for cardiovascular disease
    56.
    发明授权
    Combined F2-isoprostane and myeloperoxidase detection, a risk indicator for cardiovascular disease 有权
    联合F2-异前列烷和髓过氧化物酶检测，心血管疾病的风险指标

    公开(公告)号：US09140703B2

    公开(公告)日：2015-09-22

    申请号：US12911425

    申请日：2010-10-25

    申请人： Stanley L. Hazen ,  Renliang Zhang

    发明人： Stanley L. Hazen ,  Renliang Zhang

    IPC分类号： C12Q1/28 ,  C12Q1/26 ,  G01N33/53 ,  G01N33/573 ,  C12N1/28

    CPC分类号： G01N33/573 ,  A61K31/616 ,  C12N1/28 ,  C12Q1/28 ,  C12Y111/01007 ,  G01N2333/908 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/50 ,  G01N2800/52 ,  G06F19/00 ,  G06Q50/22 ,  G16H50/30 ,  Y02A90/22 ,  Y02A90/26

    摘要： Diagnostic tests for characterizing an individual's risk of developing or having a cardiovascular disease. In one embodiment the present diagnostic test comprises determining the level of myeloperoxidase (MPO) activity in a bodily sample obtained from the individual or test subject. In another embodiment, the diagnostic test comprises determining the level of MPO mass in a bodily sample obtained from the test subject. In another embodiment, the diagnostic test comprises determining the level of one or more select MPO-generated oxidation products in a bodily sample obtained from the test subject. The select MPO-generated oxidation products are dityrosine, nitrotyrosine, methionine sulphoxide or an MPO-generated lipid peroxidation products. Levels of MPO activity, MPO mass, or the select MPO-generated oxidation product in bodily samples from the test subject are then compared to a predetermined value that is derived from measurements of MPO activity, MPO mass, or the select MPO-generated oxidation product in comparable bodily samples obtained from healthy controls. Such comparison characterizes the test subject's risk of developing CVD.

    摘要翻译： 用于描述个体发展或患有心血管疾病的风险的诊断测试。 在一个实施方案中，本诊断测试包括确定从个体或测试对象获得的身体样品中髓过氧化物酶（MPO）活性的水平。 在另一个实施例中，诊断测试包括确定从测试对象获得的身体样本中的MPO质量的水平。 在另一个实施方案中，诊断测试包括确定从测试对象获得的身体样品中的一种或多种选择的MPO产生的氧化产物的水平。 选择的MPO生成的氧化产物是二酪氨酸，硝基酪氨酸，甲硫氨酸亚砜或MPO产生的脂质过氧化产物。 然后将来自测试受试者的身体样品中的MPO活性，MPO质量或选择的MPO生成的氧化产物的水平与从MPO活性，MPO质量或选择的MPO产生的氧化产物的测量得到的预定值进行比较 在从健康对照获得的可比较的身体样品中。 这种比较表征了测试对象发展CVD的风险。

    Rapid, low-sample-volume cholesterol and triglyceride assays
    57.
    发明授权
    Rapid, low-sample-volume cholesterol and triglyceride assays 有权
    快速，低样本量的胆固醇和甘油三酯测定

    公开(公告)号：US09051599B2

    公开(公告)日：2015-06-09

    申请号：US14100870

    申请日：2013-12-09

    申请人： Theranos, Inc.

    发明人： Douglas Matje ,  Ian Gibbons ,  Paul Patel ,  Elizabeth A. Holmes

    IPC分类号： C12Q1/60 ,  C12Q1/61 ,  G01N33/92

    CPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/61 ,  C12Q2326/00 ,  C12Q2326/90 ,  C12Q2326/96 ,  C12Q2523/115 ,  G01N33/92 ,  G01N2333/904 ,  G01N2333/908 ,  G01N2333/92

    摘要： Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.

    摘要翻译： 提供了用于从血液样品中快速测量胆固醇和胆固醇亚组分的试剂，测定法，方法，试剂盒，装置和系统。 在胆固醇亚种以不同速率转化为可检测产物的条件下，可以在单次测定中使用动力学测量来测量总胆固醇，低密度脂蛋白胆固醇和高密度脂蛋白胆固醇。 可检测的产物在测定开始后的不同时间测量。 脂肪酶，胆固醇酯酶，胆固醇氧化酶和过氧化物酶可以一起使用以产生与转化的胆固醇量成正比的有色产物。 公开了通过进一步包括甘油三酯测量来计算极低密度脂蛋白胆固醇水平的方法。 测定可以在单一反应混合物中进行，其允许更准确和精确的胆固醇测定，包括胆固醇亚部分与总胆固醇的比例，以比在不同反应混合物中进行若干不同测定所预期的更低的成本。