公开(公告)号：US09290755B2

公开(公告)日：2016-03-22

申请号：US13966880

申请日：2013-08-14

Applicant: BASF ENZYMES LLC

Inventor： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby H. Richardson ,  Paula M. Hicks

IPC: C12N9/10 ,  C12Q1/68 ,  C12P21/04 ,  C12P21/06 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C12N9/88 ,  C12N15/82 ,  C12P17/10 ,  A61K38/00

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

公开(公告)号：US20160007633A1

公开(公告)日：2016-01-14

申请号：US14770766

申请日：2014-03-10

Applicant: BASF ENZYMES LLC

Inventor： Yun HAN ,  Michael PRATT ,  Yi WU

IPC: A23K1/165 ,  B01J2/16 ,  C12N9/16

Abstract: A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C.

Abstract translation: 提供液体酶制剂，酶颗粒制剂，使用流化床干燥器制备酶颗粒的方法，其中酶颗粒是热稳定的而不需要耐热涂层。 酶颗粒是用于制造动物饲料的植酸酶颗粒，其中植酸酶颗粒是热稳定的，不需要耐热涂层，并且在80℃造粒后，植酸酶保留约63％至约134％的活性。

公开(公告)号：US09169478B2

公开(公告)日：2015-10-27

申请号：US13967157

申请日：2013-08-14

Applicant: BASF Enzymes LLC

Inventor： David P. Weiner ,  Ellen G. Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin M. Cuenca ,  Mervyn L. De Souza ,  Sherry Kollmann

IPC: C12N9/90 ,  C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C12N1/20 ,  C12N1/00 ,  C12N5/00 ,  C12N5/07 ,  C12N5/04 ,  C07H21/04 ,  C07K1/00 ,  A01K67/027 ,  C12Q1/533 ,  A23G4/12 ,  A23K1/165 ,  A23L1/03 ,  A23G3/36 ,  A23G4/14 ,  A61K38/00

CPC classification number: C12N9/90 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/10 ,  A23G3/366 ,  A23G4/123 ,  A23G4/14 ,  A23K20/189 ,  A23L29/06 ,  A23V2002/00 ,  A61K38/00 ,  A61K38/52 ,  C12Q1/533 ,  C12Y501/00 ,  C12Y501/01

Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.

Abstract translation: 本发明一般涉及酶，编码具有异构酶活性的酶的多核苷酸，例如消旋酶活性，例如氨基酸消旋酶活性，丙氨酸消旋酶活性和/或差向异构酶活性，和/或催化分子内原子重新排列 催化一种异构体转化为另一种异构体，催化光学活性底物转化为光学无活性的外消旋催化底物对映异构体的相互转化，催化仅具有一个中心的底物中不对称碳原子周围的立体化学反应 的不对称性，催化具有多于一个不对称中心的底物中不对称碳原子周围构型的立体化学反转，和/或催化氨基酸的外消旋化。

公开(公告)号：US20150125928A1

公开(公告)日：2015-05-07

申请号：US14297989

申请日：2014-06-06

Applicant: BASF ENZYMES LLC

Inventor： KELVIN WONG ,  JAY M. SHORT ,  MARK J. BURK ,  GRACE DESANTIS ,  ROBERT FARWELL ,  KELLY CHATMAN

IPC: C12N9/78

CPC classification number: C12N9/78 ,  C12N15/00 ,  C12P7/42 ,  C12P13/002 ,  C12P13/04 ,  C12P41/006 ,  C12Y305/05001 ,  Y02P20/52

Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

Abstract translation: 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提​​供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

公开(公告)号：US11530427B2

公开(公告)日：2022-12-20

申请号：US17050054

申请日：2019-05-23

Applicant: BASF SE ,  BASF Enzymes LLC

Inventor： Yukiko Sato ,  Frank Reinhold ,  Justin Andrew Bordley ,  Joseph P Borst ,  Matthew Gerard Lyon ,  Christopher Paul Myers ,  Katrina Marie Knauer

IPC: C12P7/06 ,  C12P19/14

Abstract: Disclosed herein include methods of reducing the viscosity of a starch slurry during starch processing by the addition of one or more surfactants and one or more amylases. In some embodiments, the unexpected reduction in slurry viscosity results in a surprising increase in corn oil recovery, reduction in residual starch, and increased fermentation rate.

公开(公告)号：US11447757B2

公开(公告)日：2022-09-20

申请号：US16082079

申请日：2017-03-03

Applicant: BASF Enzymes LLC

Inventor： Danielle Cusumano ,  Tony Newton ,  Arne I. Solbak

IPC: C12N9/16 ,  C12C5/00 ,  C12C11/00 ,  C12G3/02 ,  C12P7/12

Abstract: The present disclosure relates to methods for using one or more polypeptides with phytase activity in grain processing, ethanol, and biofuel production.

公开(公告)号：US20210198652A1

公开(公告)日：2021-07-01

申请号：US17110222

申请日：2020-12-02

Applicant: BASF Enzymes LLC ,  Syngenta Participations AG

Inventor： Malgorzata SLUPSKA ,  Geoff HAZLEWOOD ,  Cathy CHANG ,  Peter LUGINBUHL ,  Ellen BURKE ,  Michelle CAYOUETTE ,  Uvini GUNAWARDENA ,  Majid GHASSEMIAN ,  Aron SILVERSTONE ,  Yan ZHANG

IPC: C12N9/96 ,  C12N9/26 ,  C12N9/34

Abstract: In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.

公开(公告)号：US10982199B2

公开(公告)日：2021-04-20

申请号：US16714393

申请日：2019-12-13

Applicant: BASF Enzymes LLC

Inventor： Xuqiu Tan ,  Arne I. Solbak, Jr.

IPC: C12N9/16 ,  C12N9/24

Abstract: Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/L to 40 g/L. The phytase have higher specific activity, retain activity at low pH, and retain activity at high temperature. The phytase can be used in a variety of compositions including food, feed, pharmaceuticals, and cleaning.

公开(公告)号：US20200308558A1

公开(公告)日：2020-10-01

申请号：US16082079

申请日：2017-03-03

Applicant: BASF Enzymes LLC

Inventor： Danielle CUSUMANO ,  Tony NEWTON ,  Arne I. SOLBAK

IPC: C12N9/16 ,  C12P7/12 ,  C12C11/00 ,  C12C5/00 ,  C12G3/02

Abstract: The present disclosure relates to methods for using one or more polypeptides with phytase activity in grain processing, ethanol, and biofuel production.

公开(公告)号：US20190211291A1

公开(公告)日：2019-07-11

申请号：US16357903

申请日：2019-03-19

Applicant: DIREVO Industrial Biotechnology GmbH ,  BASF Enzymes, LLC

Inventor： Vitaly Svetlichny ,  Marco Kraemer ,  Klaudija Milos

IPC: C12F3/10 ,  A23K50/30 ,  C12P7/06 ,  C12P7/14 ,  C12N9/24 ,  C12N9/14 ,  A23K50/75 ,  A23K10/38 ,  C12C7/053 ,  C12N9/42

CPC classification number: C12F3/10 ,  A23K10/38 ,  A23K50/30 ,  A23K50/75 ,  C12C7/053 ,  C12N9/14 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2482 ,  C12N9/2491 ,  C12P7/06 ,  C12P7/14 ,  C12Y302/01004 ,  C12Y302/01015 ,  Y02E50/17 ,  Y02P60/873

Abstract: A method of recovering oil, which includes (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism, wherein the fermentation medium comprises a xylanase and a pectinase; (d) distilling the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; and (f) recovering the oil from the thin stillage.