公开(公告)号：US20190284579A1

公开(公告)日：2019-09-19

申请号：US16426310

申请日：2019-05-30

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Don Masquelier ,  Phillip Belgrader ,  Kevin Ness

IPC: C12N15/87 ,  C12M1/42 ,  C12N15/70 ,  C12M3/00 ,  C12N15/81 ,  C12M1/36

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments comprising one or more flow-through electroporation devices or modules are provided to automate genome editing in live cells.

公开(公告)号：US10415058B2

公开(公告)日：2019-09-17

申请号：US16147871

申请日：2018-09-30

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Don Masquelier ,  Phillip Belgrader

IPC: C12M3/00 ,  B01L3/00 ,  C12N15/87 ,  C12M1/42 ,  C12N15/70 ,  C12N15/81 ,  C12M1/36

Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.

公开(公告)号：US20190185846A1

公开(公告)日：2019-06-20

申请号：US16269671

申请日：2019-02-07

Applicant: Inscripta, Inc.

Inventor： Don Masquelier ,  Phillip Belgrader ,  Jorge Bernate ,  Ryan Gill ,  Kevin Ness

IPC: C12N15/10 ,  C40B40/02 ,  C12M1/36 ,  C12N15/11 ,  C12M3/00 ,  C12M1/12 ,  C12M1/00 ,  C12N9/22

CPC classification number: C12N15/1082 ,  C12M23/44 ,  C12M33/14 ,  C12M35/00 ,  C12M37/04 ,  C12M41/36 ,  C12M41/48 ,  C12M43/00 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2800/80 ,  C40B40/02

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

公开(公告)号：US10323258B2

公开(公告)日：2019-06-18

申请号：US16147865

申请日：2018-09-30

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Don Masquelier ,  Phillip Belgrader ,  Kevin Ness

IPC: C12N15/00 ,  C12M3/00 ,  B01L3/00 ,  C12N15/87 ,  C12M1/42 ,  C12N15/70 ,  C12N15/81 ,  C12M1/36

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments comprising one or more flow-through electroporation devices or modules are provided to automate genome editing in live cells.

公开(公告)号：US20250084392A1

公开(公告)日：2025-03-13

申请号：US18725912

申请日：2023-01-04

Applicant: INSCRIPTA, INC.

Inventor： Brian Chaikind ,  Christopher Lim

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: This invention relates to compositions of matter, methods and instruments for directly recruiting repair templates to CRISPR nucleases to stimulate homology-directed repair. Molecular “tethers” are described which result in an increase in the local concentration of repair templates at the site of the double-strand break made by a nuclease, thereby enhancing the rate of homology directed repair and suppressing undesired edits.

公开(公告)号：US20250043314A1

公开(公告)日：2025-02-06

申请号：US18717586

申请日：2022-12-19

Applicant: Inscripta, Inc.

Inventor： Erik Zimmerman ,  Emily Feldman ,  Christian Siltanen ,  Marina Barreto Felisbino

IPC: C12N15/90 ,  C12N9/12 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6869

Abstract: The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion enzyme editing of nucleic acids in live mammalian cells, and for tracking of editing events.

公开(公告)号：US12195775B2

公开(公告)日：2025-01-14

申请号：US18117082

申请日：2023-03-03

Applicant: Inscripta, Inc.

Inventor： Amanda Reider Apel ,  Karolina Kalbarczyk ,  Drew Fraser Thacker ,  Abhinav Kumar

IPC: C12N9/90 ,  C12N1/18 ,  C12N1/20 ,  C12P7/22 ,  C12R1/19 ,  C12R1/865

Abstract: The present disclosure relates to synthetic biology and, in particular, the bioproduction of bakuchiol, and engineered enzymes for producing the same.

公开(公告)号：US20240318110A1

公开(公告)日：2024-09-26

申请号：US18681313

申请日：2022-08-04

Applicant: INSCRIPTA, INC.

Inventor： Andrea BRYAN ,  Bruce CHABANSKY ,  David STUMBO ,  Eric SMITH ,  Jorge BERNATE ,  Diane BRANNIGAN

IPC: C12M1/00 ,  C12M1/06 ,  C12M1/12 ,  C12M1/34

CPC classification number: C12M23/58 ,  C12M23/38 ,  C12M25/02 ,  C12M27/06 ,  C12M29/04 ,  C12M41/12 ,  C12M41/36

Abstract: This invention relates to modules and automated, integrated, end-to-end closed instruments for automated mammalian cell growth and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：US11965186B2

公开(公告)日：2024-04-23

申请号：US17676218

申请日：2022-02-20

Applicant: Inscripta, Inc.

Inventor： Juhan Kim ,  Benjamin Mijts

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N15/90

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  C12Y301/21004 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure provides engineered nucleic acid-guided nickases and optimized scaffolds for making rational, direct edits to nucleic acids in live cells.

公开(公告)号：US11891609B2

公开(公告)日：2024-02-06

申请号：US17680289

申请日：2022-02-25

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

CPC classification number: C12N15/74 ,  C12N1/20 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/20

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.