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    FUNGAL DELTA-12 DESATURASE AND DELTA-15 DESATURASE MOTIFS
    61.
    发明申请
    FUNGAL DELTA-12 DESATURASE AND DELTA-15 DESATURASE MOTIFS 审中-公开
    真菌DELTA-12 DESATURASE和DELTA-15去饱和运动

    公开(公告)号：US20090186362A1

    公开(公告)日：2009-07-23

    申请号：US12396845

    申请日：2009-03-03

    申请人： NARENDRA S. YADAV ,  HONGXIANG ZHANG

    发明人： NARENDRA S. YADAV ,  HONGXIANG ZHANG

    IPC分类号： C12Q1/68 ,  C12Q1/26 ,  C12N9/02 ,  C40B30/04 ,  C12P7/64

    CPC分类号： C12P7/6427 ,  C12N9/001 ,  C12N9/0083 ,  C12P7/6472 ,  C12Q1/6895

    摘要： The present invention relates to fungal Δ12 desaturases (responsible for conversion of oleic acid to linoleic acid (18:2, LA)) and Δ15 fatty acid desaturases (responsible for conversion of LA to α-linolenic acid (18:3, ALA)). Amino acid motifs diagnostic of Δ12 desaturases and Δ15 desaturases are also provided. Methods of altering enzyme specificity towards Δ12 desaturation or towards Δ15 desaturation and/or increasing production of specific ω-3 and ω-6 fatty acids by over-expression of the fungal desaturases are also described.

    摘要翻译： 本发明涉及真菌Delta12去饱和酶（负责将油酸转化为亚油酸（18:2，LA））和Δ15脂肪酸去饱和酶（负责将LA转化成α-亚麻酸（18:3，ALA）） 。 还提供了Delta12去饱和酶和Δ15去饱和酶的氨基酸图案诊断。 还描述了通过过度表达真菌去饱和酶改变酶特异性对Delta12去饱和或Delta5去饱和和/或增加特定ω-3和ω-6脂肪酸的产生的方法。

    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    63.
    发明授权
    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权
    高山被孢霉二酰基甘油酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号：US07198937B2

    公开(公告)日：2007-04-03

    申请号：US11024545

    申请日：2004-12-29

    申请人： Zhixiong Xue ,  Narendra S. Yadav ,  Daniel Joseph Macool

    发明人： Zhixiong Xue ,  Narendra S. Yadav ,  Daniel Joseph Macool

    IPC分类号： C12N1/16 ,  C12N1/13 ,  C12N1/19 ,  C12N1/18 ,  C12N9/10 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

    摘要： An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    摘要翻译： 提供了一种酰基转移酶，适用于制造含油生物体中富含ω-脂肪酸的微生物油。 具体地，从高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT2）的基因。 该基因编码参与真菌和酵母中油生物合成的最终步骤的酶，并且预期在改变油性生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 最理想的是，即时DGAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累长链等于或大于C 20的长链PUFA。

    Binary viral expression system in plants
    65.
    发明授权
    Binary viral expression system in plants 失效
    植物二元病毒表达系统

    公开(公告)号：US06632980B1

    公开(公告)日：2003-10-14

    申请号：US09442021

    申请日：1999-11-17

    申请人： Narendra S. Yadav ,  S. Carl Falco

    发明人： Narendra S. Yadav ,  S. Carl Falco

    IPC分类号： C12N1582

    CPC分类号： C07K14/005 ,  A61K47/60 ,  A61K47/62 ,  C07K17/08 ,  C12N15/8203 ,  C12N15/8209 ,  C12N15/8213 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8222 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8289 ,  C12N2750/12022 ,  C12N2770/00022 ,  C12N2770/40022

    摘要： The invention relates to two plant transgene expression systems. The first is comprised of two chromosomally-integrated components that are individually heritable. One component is an inactive replicon, which contains cis-acting viral sequences required for replication and is unable to replicate episomally. The other component is a chimeric transactivating gene comprising a regulated promoter operably-linked to the coding region for a protein that can transactivate replicon replication. Regulated expression of the transactivation protein in plant cells containing the inactive replicon triggers release of free replicon from the integrated inactive replicon and allows episomal replication.

    摘要翻译： 本发明涉及两种植物转基因表达系统。 第一个由两个独立于遗传的染色体整合成分组成。 一个组分是一个非活性的复制子，其中包含复制所需的顺式作用病毒序列，并且无法以阴离子型复制。 另一组分是嵌合反式激活基因，其包含可操作地连接到可以反式复制子复制的蛋白质的编码区的调节启动子。 含有非活性复制子的植物细胞中的反式激活蛋白的调节表达引发了来自整合的无活性复制子的游离复制子的释放并允许附加型复制。

    DELTA-15 DESATURASES SUITABLE FOR ALTERING LEVELS OF POLYUNSATURATED FATTY ACIDS IN OILSEED PLANTS AND OLEAGINOUS YEAST
    67.
    发明申请
    DELTA-15 DESATURASES SUITABLE FOR ALTERING LEVELS OF POLYUNSATURATED FATTY ACIDS IN OILSEED PLANTS AND OLEAGINOUS YEAST 审中-公开
    DELTA-15适用于改变油性植物和油脂酵母中多糖脂肪酸含量的食物

    公开(公告)号：US20140050838A1

    公开(公告)日：2014-02-20

    申请号：US13897556

    申请日：2013-05-20

    申请人： HOWARD GLENN DAMUDE ,  NARENDRA S. YADAV

    发明人： HOWARD GLENN DAMUDE ,  NARENDRA S. YADAV

    IPC分类号： A23D9/04 ,  C11B1/00

    CPC分类号： C12N9/0083 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

    摘要： The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.

    摘要翻译： 本发明涉及能够催化亚油酸（18:2，LA）转化为α-亚麻酸（18:3，ALA）的真菌Δ-15脂肪酸去饱和酶。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主植物和微生物。 本文还描述了通过过表达Δ-15脂肪酸去饱和酶增加特定ω-3和ω-6脂肪酸的产生的方法。