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    MODIFIED NUCLEOTIDE LINKERS
    63.
    发明申请
    MODIFIED NUCLEOTIDE LINKERS 有权

    公开(公告)号：US20220186306A1

    公开(公告)日：2022-06-16

    申请号：US17562510

    申请日：2021-12-27

    申请人： Illumina Cambridge Limited

    发明人： Xiaolin Wu ,  Xiaohai Liu

    IPC分类号： C12Q1/6869 ,  C07H19/14

    摘要： Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.

    Modified nucleotides
    69.
    发明授权
    Modified nucleotides 有权

    公开(公告)号：US10513731B2

    公开(公告)日：2019-12-24

    申请号：US15179813

    申请日：2016-06-10

    申请人： Illumina Cambridge Limited

    发明人： John Milton ,  Xiaolin Wu ,  Mark Smith ,  Joseph Brennan ,  Colin Barnes ,  Xiaohai Liu ,  Silke Ruediger

    IPC分类号： C12Q1/6869 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H1/00 ,  C07H19/14

    摘要： The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3′-OH blocking group covalently attached thereto, such that the 3′ carbon atom has attached a group of the structure —O—Z wherein Z is any of —C(R′)2-O—R″, —C(R′)2-N(R″)2, —C(R′)2-N(H)R″, —C(R′)2-S—R″ and —C(R′)2-F, wherein each R″ is or is part of a removable protecting group; each R′ is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R′)2 represents an alkylidene group of formula ═C(R′″)2 wherein each R′″ may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R″ is exchanged for H or, where Z is —C(R′)2-F, the F is exchanged for OH, SH or NH2, preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3′OH; with the proviso that where Z is —C(R′)2-S—R″, both R′ groups are not H.