公开(公告)号：US06624408B1

公开(公告)日：2003-09-23

申请号：US09407729

申请日：1999-09-28

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： B01D5944

CPC分类号： H01J49/0036

摘要： The invention relates to the scanning and representation of daughter ion spectra for the purpose of determining the structural characteristics of parent ions in ion traps. The invention consists of combining all or selected daughter and granddaughter spectra of a parent ion over several generations in one combined descendants spectrum. This combined descendants spectrum can be depicted as a graphic or a list. The references to origin can be plotted on the combined descendants spectrum. For biopolymers, where the loss of fragments can be identified due to their mass, the names or abbreviations of lost molecule fragments can be entered. The criteria for selection of the spectra can be predefined; in this way, the spectra can be depicted and even scanned automatically.

摘要翻译： 本发明涉及扫描和表示子离子谱，以确定离子阱中母体离子的结构特征。 本发明包括在一个组合的后代谱中结合几代的母体离子的所有或选定的女儿和孙女光谱。 这种组合的后代谱可以被描绘成图形或列表。 对起源的引用可以绘制在组合的后代谱上。 对于生物聚合物，其中由于其质量可以鉴定片段的损失，可以输入丢失的分子片段的名称或缩写。 选择光谱的标准可以预先定义; 以这种方式，可以自动绘制和均匀扫描光谱。

公开(公告)号：US06555814B1

公开(公告)日：2003-04-29

申请号：US09607774

申请日：2000-06-30

申请人： Gökhan Baykut ,  Jochen Franzen

发明人： Gökhan Baykut ,  Jochen Franzen

IPC分类号： B01D5944

CPC分类号： H01J49/063 ,  H01J49/38 ,  H01J49/4265

摘要： The invention relates to a method and a device for controlling the number of ions in ion cyclotron resonance (ICR) mass spectrometers, whereby the ions enter a multipole ion guide after their formation and are stored there temporarily. By measuring the ion number in a predefined subset of these temporarily stored ions, the number of ions transferred into the ICR trap for mass spectrometric analysis is regulated. A mode of operation of the multipole ion guide can ensure that undesirable mass ranges are filtered out before the transfer of ions into the ICR mass spectrometer. The invention makes it possible to eliminate space charge effects, which are caused by overfilling the ICR traps.

摘要翻译： 本发明涉及一种用于控制离子回旋共振（ICR）质谱仪中的离子数的方法和装置，由此离子在其形成后进入多极离子导向器并暂时储存在其中。 通过测量这些临时存储的离子的预定子集中的离子数，调节转移到用于质谱分析的ICR阱中的离子数。 多极离子导向器的操作模式可以确保在将离子转移到ICR质谱仪之前过滤不需要的质量范围。 本发明使得可以消除由过度填充ICR陷阱引起的空间电荷效应。

公开(公告)号：US06180372B2

公开(公告)日：2001-01-30

申请号：US09049646

申请日：1998-03-27

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： C12P1934

CPC分类号： B01L3/502707 ,  B01L7/52 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0487 ,  B01L2400/084 ,  C12Q1/686 ,  Y10S977/775 ,  Y10S977/783 ,  Y10S977/788 ,  Y10S977/80 ,  Y10S977/924 ,  C12Q2565/629 ,  C12Q2527/125 ,  C12Q2527/113

摘要： The invention concerns methods and instruments for fast, selective replication of deoxyribonucleic acid (DNA) from biomaterial through the known polymerase chain reaction (PCR), working in individual duplication thermocycles. The invention consists of extremely brief cycle times of only a few seconds for the PCR reactions, generated, on the one hand, by reaction chambers for the reception of the reaction solution constructed of a pattern of fine capillaries in close proximity to heating and cooling elements in order to optimally accelerate the temperature setting in the reaction solution for the three temperature phases of the PCR duplication cycles and, on the other hand, by keeping the flow rates in the capillaries to a minimum during the amplification phase so that the polymerase reaction is not disturbed. The capillary pattern can be simply produced by means of microsystern technology.

摘要翻译： 本发明涉及通过已知的聚合酶链式反应（PCR）从生物材料快速，选择性复制脱氧核糖核酸（DNA）的方法和工具，在单独的复制热循环中工作。 本发明由PCR反应的极短的循环时间仅为几秒钟，一方面由反应室产生，用于接收由加热和冷却元件附近的精细毛细管图案构成的反应溶液 为了最佳地加速PCR复制循环的三个温度阶段的反应溶液中的温度设定，另一方面，通过在扩增阶段将毛细管中的流速保持在最小值，使得聚合酶反应为 不打扰 毛细管图案可以通过微观技术简单地生产。

公开(公告)号：US6049077A

公开(公告)日：2000-04-11

申请号：US133868

申请日：1998-08-13

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： H01J49/40

CPC分类号： H01J49/40

摘要： The time-of-flight mass spectrometers which must demonstrate a high constancy of the calibrated mass scale even under changeable ambient temperatures and thermal loads due to pumps or electronics. Time-of-flight mass spectrometers calculate the masses of ions from the measured time of flight in a long flight tube that is normally manufactured of stainless steel. These flight tubes are subject to temperature-related length changes which affect the flight time and therefore the mass determination. The thermal expansion of spectrometer parts between ion source and ion detector, thus keeping the flight path for the ions at a constant length. Length compensation can be produced by design of the spacing system made of materials of different thermal expansion coefficients, the length changes of which balance out in opposite directions.

摘要翻译： 飞行时间质谱仪即使在可变环境温度和由于泵或电子设备造成的热负载的情况下也必须表现出校准质量标度的高恒定性。 飞行时间质谱仪从通常由不锈钢制造的长飞行管中测量的飞行时间计算离子质量。 这些飞行管受到温度相关的长度变化，影响飞行时间，因此影响质量测定。 离子源和离子检测器之间的光谱仪部分的热膨胀，从而保持离子的飞行路径恒定的长度。 长度补偿可以通过设计由不同热膨胀系数的材料制成的间隔系统，其长度变化在相反方向平衡。

公开(公告)号：US5969348A

公开(公告)日：1999-10-19

申请号：US933862

申请日：1997-09-19

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： H01J49/40 ,  B01D59/44 ,  H01J49/00

CPC分类号： H01J49/403

摘要： The invention relates to measurement methods for time-of-flight mass spectrometers which operate with an ionization of analyte substances adsorbed at the surface of a sample support and an improvement in mass resolution through delayed ion acceleration (or "delayed extraction") in front of the sample support. It particularly relates to velocity focusing for good mass resolution simultaneously for wide ranges of masses within the spectrum.The invention consists of focusing the flight-times of the ions simultaneously for all masses in wide ranges of interest relative to their initial velocity, by allowing the acceleration in the first accelerating region to increase in time after being switched on. Thus a good resolution cannot only be set for one mass on the spectrum but for all masses in wide ranges simultaneously. In computer simulations, provided there is a correlation of space and velocity distribution, focusing of a least first order is obtained simultaneously for all ions.

摘要翻译： 本发明涉及飞行时间质谱仪的测量方法，其通过吸附在样品载体表面的分析物质的离子化和通过延迟离子加速（或“延迟提取”）提高质量分辨率而工作， 样品支持。 它特别涉及速度聚焦，用于在光谱范围内的宽范围的质量范围内同时获得良好的质量分辨率。 本发明包括通过允许第一加速区域中的加速度在接通之后随时间增加，将离子的飞行时间同时聚焦在相对于其初始速度的广泛范围内的所有质量。 因此，不仅可以在光谱上设置一个质量，而且可以同时为宽范围内的所有质量设置好的分辨率。 在计算机模拟中，只要存在空间和速度分布的相关性，所有离子同时获得最小一阶的聚焦。

公开(公告)号：US5936241A

公开(公告)日：1999-08-10

申请号：US32579

申请日：1998-02-27

申请人： Jochen Franzen ,  Michael Schubert

发明人： Jochen Franzen ,  Michael Schubert

IPC分类号： H01J49/42

CPC分类号： H01J49/4265

摘要： The invention consists of deriving the control of the space charge in the ion trap for the initial daughter ion spectrum from the filling rates of previous normal spectra, from the abundance ratio of the parent ions to be isolated to the total ions in the spectrum, and from the at least roughly known isolation and fragmentation yields. For further daughter ion spectra, the resulting measured overall filling rate with daughter ions may be used. The same applies in an analogue way to spectra of isolated ions or of ions from MS.sup.n processes.

摘要翻译： 本发明包括从先前正常光谱的填充率，从要分离的母离子的丰度比与光谱中的总离子的丰度比，导出离子阱中离子阱中空间电荷的控制，以及 从至少大致已知的分离和碎裂产率。 对于更多的子离子光谱，可以使用与子离子的测得的总体填充率。 同样的情况也适用于分离离子或MSn过程离子的光谱。

公开(公告)号：US5898174A

公开(公告)日：1999-04-27

申请号：US925629

申请日：1997-09-09

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： H01J49/16 ,  H01J49/40

CPC分类号： H01J49/40

摘要： A linear time-of-flight mass spectrometer which operates using ionization of analyte substances adsorbed on a sample support plate and an improvement in mass resolution through delayed acceleration of the ions in front of the sample support plate. The geometric design of the mass spectrometer consists of focusing the flight time of the ions in second or higher order by maintaining geometric requirements for the lengths of acceleration paths in the ion source relative to the field-free flight path length. In computer simulations, resolutions of flight time greater than one million have been obtained even for very high ion masses provided there is a correlation in space and velocity distribution when switching on the acceleration.

摘要翻译： 线性飞行时间质谱仪，其使用吸附在样品支持板上的分析物质的电离进行操作，并且通过样品支撑板前面的离子的延迟加速提高质量分辨率。 质谱仪的几何设计包括通过保持离子源相对于无场飞行路径长度的加速度路径的长度的几何要求，将离子的飞行时间以二次或更高次序进行聚焦。 在计算机模拟中，即使对于非常高的离子质量，飞行时间大于一百万的分辨率也可以获得，只要开启加速度时空间和速度分布有相关性。

公开(公告)号：US5763878A

公开(公告)日：1998-06-09

申请号：US620803

申请日：1996-03-28

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： H01J49/04 ,  H01J49/40 ,  H01J49/42 ,  B01D59/44 ,  H01J49/00

CPC分类号： H01J49/423 ,  H01J49/401

摘要： The invention relates to methods and devices for the orthogonal injection of ions into a time-of-flight mass spectrometer, whereby the ions preferably originate from ion sources which are located outside of the vacuum system of the mass spectrometer. The invention consists of first introducing the ions into a multipole rod arrangement with extended pole rods which stretches orthogonally to the flight direction of the ions in the time-of-flight spectrometer, and then outpulsing the ions by means of a rapid change of the electrical field, perpendicular to the rod direction, through the intermediate space between two rods. The multipole arrangement can take the form of an ion storage device by fitting reflectors to the ends. The multipole arrangement can be filled with the aid of another multipole arrangement which takes the form of an ion guide. Damping of the ion oscillations with the aid of a collision gas leads to a collection of ions in a very thin thread on the axis of the multipole arrangement, providing the time-of-flight spectrometer with an excellent mass resolving power due to the uniform initial energy and low energy spread of the ions.

摘要翻译： 本发明涉及用于将离子正交注入飞行时间质谱仪的方法和装置，其中离子优选源自位于质谱仪真空系统外部的离子源。 本发明包括首先将离子引入具有延伸极杆的多极杆装置中，其延伸的极杆与飞行时间分光计中的离子的飞行方向正交地延伸，然后通过电气的快速变化使离子脉冲离开 垂直于杆方向，通过两个杆之间的中间空间。 通过将反射器装配到端部，多极排列可以采用离子存储装置的形式。 可以借助于采用离子导向器形式的另一多极装置来填充多极装置。 借助碰撞气体对离子振荡的阻尼导致多极排列轴线上非常薄的螺纹中的离子的集合，为飞行时间谱仪提供了优异的质量分辨能力，由于均匀的初始 能量和低能量的离子扩散。

公开(公告)号：US5608217A

公开(公告)日：1997-03-04

申请号：US402125

申请日：1995-03-10

申请人： Jochen Franzen ,  Matthias Mann ,  Matthias Wilm

发明人： Jochen Franzen ,  Matthias Mann ,  Matthias Wilm

IPC分类号： G01N30/28 ,  G01N30/72 ,  H01J49/04 ,  H01J49/42 ,  H01J49/10

CPC分类号： H01J49/165 ,  G01N30/7266 ,  G01N2030/285

摘要： A method is provided for analyzing analyte ions in a liquid using a spectrometer incorporating an ion trap, using a capillary nozzle adjacent an inlet aperture for the ion trap. The liquid is electrosprayed from the capillary nozzle by generating an electric field at the nozzle to ionise the said analyte and produce a beam of charged spray particles, and the beam of charged particles is injected into the ion trap through the inlet aperture. The pressure within the capillary is such that flow of the liquid through the capillary occurs only in the presence of the said electric field, and flow of ions into the ion trap is controlled by varying the electric field.

摘要翻译： 提供了一种使用包含离子阱的光谱仪来分析液体中的分析物离子的方法，其使用与离子阱的入口孔相邻的毛细喷嘴。 通过在喷嘴处产生电场来离子化所述分析物并产生一束带电的喷雾颗粒，从毛细喷嘴喷射液体，并将带电粒子束通过入口孔注入离子阱。 毛细管内的压力使得液体通过毛细管的流动仅在存在所述电场的情况下发生，并且通过改变电场来控制离子流入离子阱的流量。

公开(公告)号：US5595636A

公开(公告)日：1997-01-21

申请号：US401404

申请日：1995-03-09

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： H01J49/0418 ,  G01N27/44717

摘要： The invention relates to the mass spectrometric analysis of separated substance samples on so-called 2-D-gel electrophoresis plates, used particularly for protein determination, with ionization of the substance samples by MALDI. The molecules of the substance samples are transferred to a thin, lacquer-like smooth matrix layer which should preferably be applied to a metal sample support. Transfer takes place directly or indirectly, for example via a polyvinylidene difluoride membrane an an intermediate carrier, by electrophoretic transport of the molecules to the matrix surface. Before transfer, the proteins may be subjected to enzymatic cutting of their amino acid chains.

摘要翻译： 本发明涉及所谓的2-D凝胶电泳板上分离的物质样品的质谱分析，特别用于蛋白质测定，通过MALDI离子化物质样品。 将物质样品的分子转移到薄的，类似漆的光滑基质层，其优选应用于金属样品载体。 转移通过例如通过聚偏二氟乙烯膜和中间载体直接或间接进行，通过将分子电泳转运到基质表面。 在转移之前，蛋白质可以进行酶切割其氨基酸链。