摘要:
A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.
摘要:
An immiscible mixture of wax and silicone oil is provided for application to the inner surface of a thermocycle reactor tube. The mixture is a solid at typical room temperatures and under typical product storage conditions, but at temperatures above the melting point of the mixture (e.g., >46° C. and therefore, at or above 90° C. (which is typical of the first stage of a PCR thermocycle)) the mixture melts and covers the exposed surface of the solution undergoing the thermocycle, thereby sealing the reaction against evaporation and/or condensation over the duration of the multiple thermocycles typical of a PCR reaction. Upon cooling of the reaction tube, the resulting amplicons can then extracted from below the sealing layer.
摘要:
A system for charging a battery pack of an electric vehicle comprises a heater for pre-heating the battery pack so that the battery pack is able to accept a charge from a charger. The battery pack is selectively de-coupled from the system during the pre-heating. When the battery pack has reached an appropriate temperature, the heater is selectively de-coupled from the system and the charger is coupled to the system to charge the battery. Advantageously, the battery pack is protected during pre-heating.
摘要:
The systems and methods described herein include a microfluidic chip having a plurality of microfeatures interconnected to provide a configurable fluid transport system for processing at least one reagent. Inserts are provided to removably interfit into one or more of the microfeatures of the chip, wherein the inserts include sites for interactions with the reagent. As will be seen from the following description, the microfluidic chip and the inserts provide an efficient and accurate approach for conducting parallel assays.
摘要:
A method for accessing VPN using PW Emulation Edge-to-Edge Virtual Leased Line includes: configuring a first PW ID in a first Provider Edge Device that hosts a VSI, and an IP address of a second Provider Edge Device that hosts an PW Emulation Edge-to-Edge Virtual Leased Line associated with the PW ID; sending a second PW ID of the second Provider Edge Device to the first Provider Edge Device; determining whether the second PW ID of the second Provider Edge Device matches the first PW ID of the first Provider Edge Device, if it matches, setting the state of the PW between the first Provider Edge Device and the second Provider Edge Device; delivering a Forwarding Table by the first Provider Edge Device; establishing a bi-directional connection between the PW Emulation Edge-to-Edge Virtual Leased Line and the VSI. A Virtual Private System, a first Provider Edge Device, and a second Provider Edge Device are also provided.
摘要:
A method of reducing processing of fast inverse transform of an input transform block by a video decoder includes determining whether a block type is one of zero, DC, left, and top. If not, the inverse transform is performed and a residual video block is provided as residual information. When the block type is zero, inverse transform is bypassed. When the block type is DC, reduced complexity inverse transform of a DC coefficient is performing and a single residual coefficient is provided as residual information. When the block type is left, reduced complexity inverse transform of a left column of the input transform block is performed and a single column of residual coefficients is provided as residual information. When the block type is top, reduced complexity inverse transform of a top row is performed and a single row of residual coefficients is provided as residual information.
摘要:
An structure and method of manufacturing a microstructure for use in a heat exchanger is disclosed. The heat exchanger comprises a manifold layer and an microstructured region. The manifold layer comprises a structure to deliver fluid to the microstructured region. The microstructured region is formed from multiple windowed layers formed from heat conductive layers through which a plurality of microscaled apertures have been formed by a wet etching process. The plurality of windowed layers are then coupled together to form a composite microstructure.
摘要:
An efficient thermal management system (100) that utilizes a single heat exchanger (133) is provided. A refrigeration subsystem (103) cools the heat exchanger (133). A first coolant loop (139) in thermal communication with the heat exchanger (133) is used to cool the energy storage system (137). A second coolant loop (151) corresponding to the HVAC subsystem (107) is also in thermal communication with the heat exchanger (133). Preferably a third coolant loop (109) corresponding to the drive motor cooling subsystem (101) is coupleable to the HVAC coolant loop (151), thus providing an efficient means of providing heat to the HVAC subsystem (107).
摘要:
A method for making a polymeric microfluidic structure in which two or more components (layers) of the microfluidic structure are fixedly bonded or laminated with a weak solvent bonding agent, particularly acetonitrile or a mixture of acetonitrile and alcohol. In an aspect, acetonitrile can be used as a weak solvent bonding agent to enclose a microstructure fabricated in or on a non-elastomeric polymer such as polystyrene, polycarbonate, acrylic or other linear polymer to form a three-dimensional microfluidic network. The method involves the steps of wetting at least one of the opposing surfaces of the polymeric substrate components with the weak solvent bonding agent in a given, lower temperature range, adjacently contacting the opposing surfaces, and thermally activating the bonding agent at a higher temperature than the lower temperature range for a given period of time. The contacted polymeric substrates may also be aligned prior to thermal activation and compressed during thermal activation. A laminated, polymeric microfluidic structure is also disclosed.