公开(公告)号：US08647337B2

公开(公告)日：2014-02-11

申请号：US12489875

申请日：2009-06-23

申请人： R. Sam Niedbala ,  Lincoln C. Young ,  Peng Zhou

发明人： R. Sam Niedbala ,  Lincoln C. Young ,  Peng Zhou

IPC分类号： A61B18/18 ,  F17C7/02 ,  F17C13/00

CPC分类号： A61B18/0218 ,  A61B2018/0275

摘要： A dispensing head for dispensing a cryogenic fluid may comprise a flow passage configured to be placed in flow communication with a reservoir containing a cryogenic fluid, the flow passage defining a flow passage inlet opening configured to receive the cryogenic fluid from the reservoir, and a flow passage outlet opening opposite the flow passage inlet opening. The dispensing head may further comprise a dispensing member configured to dispense the cryogenic fluid, the dispensing member defining a lumen having a lumen inlet opening and a lumen outlet opening; and at least one porous member disposed in the flow passage, the at least one porous member being configured as a primary flow regulation mechanism to limit a flow rate of the cryogenic fluid as it flows from the reservoir to the lumen outlet opening.

摘要翻译： 用于分配低温流体的分配头可以包括流动通道，其构造成与包含低温流体的储存器流动连通，流动通道限定被构造成从储存器接收低温流体的流动通道入口开口， 通道出口与流路入口相对。 分配头还可以包括配置成分配低温流体的分配构件，分配构件限定具有管腔入口和管腔出口的内腔; 以及至少一个多孔构件，其设置在所述流动通道中，所述至少一个多孔构件被构造为主流量调节机构，以限制所述低温流体从储存器流到管腔出口开口的流量。

公开(公告)号：US08383039B2

公开(公告)日：2013-02-26

申请号：US13033165

申请日：2011-02-23

申请人： Peng Zhou ,  Lincoln C. Young ,  Benjamin Thomas ,  Zongyuan Chen ,  Greg Mouchka ,  Todd Roswech ,  Gwendolyn Spizz ,  Rubina Yasmin

发明人： Peng Zhou ,  Lincoln C. Young ,  Benjamin Thomas ,  Zongyuan Chen ,  Greg Mouchka ,  Todd Roswech ,  Gwendolyn Spizz ,  Rubina Yasmin

IPC分类号： G01N21/00 ,  B01L3/02

CPC分类号： C12Q1/6806 ,  B01L3/022 ,  B01L3/5027 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2200/142 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0666 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/708 ,  G01N35/1002 ,  C12Q2565/629

摘要： A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.

公开(公告)号：US20120279638A1

公开(公告)日：2012-11-08

申请号：US13555506

申请日：2012-07-23

申请人： Peng Zhou ,  Lincoln C. Young

发明人： Peng Zhou ,  Lincoln C. Young

IPC分类号： B32B37/02 ,  B32B37/10 ,  B32B37/12

CPC分类号： B32B37/0038 ,  B01F5/0683 ,  B01F5/0688 ,  B01F11/0071 ,  B01F13/0059 ,  B01L3/502707 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/084 ,  B29C65/4895 ,  B29C66/1122 ,  B29C66/54 ,  B29C66/543 ,  B29C66/71 ,  B29C66/83411 ,  B29C66/8362 ,  B29L2031/756 ,  F04B43/043 ,  F04B43/06 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0021 ,  F16K99/0034 ,  F16K99/0055 ,  F16K99/0057 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T156/1002 ,  B29K2069/00 ,  B29K2033/08 ,  B29K2025/06

摘要： Plastic microfluidic structures having a substantially rigid diaphragm that actuates between a relaxed state wherein the diaphragm sits against the surface of a substrate and an actuated state wherein the diaphragm is moved away from the substrate. As will be seen from the following description, the microfluidic structures formed with this diaphragm provide easy to manufacture and robust systems, as well readily made components such as valves and pumps.

摘要翻译： 塑料微流体结构具有基本上刚性的膜片，其在松弛状态之间起作用，其中隔膜抵靠衬底的表面，以及致动状态，其中隔膜移动离开衬底。 从以下描述可以看出，用这种隔膜形成的微流体结构提供易于制造和坚固的系统，以及易于制造的部件，例如阀和泵。

公开(公告)号：US20100319360A1

公开(公告)日：2010-12-23

申请号：US12489875

申请日：2009-06-23

申请人： R. Sam NIEDBALA ,  Lincoln C. Young ,  Peng Zhou

发明人： R. Sam NIEDBALA ,  Lincoln C. Young ,  Peng Zhou

IPC分类号： F17C7/02

CPC分类号： A61B18/0218 ,  A61B2018/0275

摘要： A dispensing head for dispensing a cryogenic fluid may comprise a flow passage configured to be placed in flow communication with a reservoir containing a cryogenic fluid, the flow passage defining a flow passage inlet opening configured to receive the cryogenic fluid from the reservoir, and a flow passage outlet opening opposite the flow passage inlet opening. The dispensing head may further comprise a dispensing member configured to dispense the cryogenic fluid, the dispensing member defining a lumen having a lumen inlet opening and a lumen outlet opening; and at least one porous member disposed in the flow passage, the at least one porous member being configured as a primary flow regulation mechanism to limit a flow rate of the cryogenic fluid as it flows from the reservoir to the lumen outlet opening.

摘要翻译： 用于分配低温流体的分配头可以包括流动通道，其构造成与包含低温流体的储存器流动连通，流动通道限定被构造成从储存器接收低温流体的流动通道入口开口， 通道出口与流路入口相对。 分配头还可以包括配置成分配低温流体的分配构件，分配构件限定具有管腔入口和管腔出口的内腔; 以及至少一个多孔构件，其设置在所述流动通道中，所述至少一个多孔构件被构造为主流量调节机构，以限制所述低温流体从储存器流到管腔出口开口的流量。

公开(公告)号：US20090056822A1

公开(公告)日：2009-03-05

申请号：US12248496

申请日：2008-10-09

申请人： Lincoln C. Young ,  Peng Zhou

发明人： Lincoln C. Young ,  Peng Zhou

IPC分类号： F15C3/04

CPC分类号： F16K7/12 ,  B01F5/0683 ,  B01F5/0688 ,  B01F11/0071 ,  B01F13/0059 ,  B01F2215/0034 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/084 ,  B29C65/4895 ,  B29C66/004 ,  B29C66/54 ,  B29C66/5412 ,  B29C66/71 ,  B29K2025/00 ,  B29K2025/04 ,  B29K2033/08 ,  B29K2069/00 ,  B29K2101/12 ,  B29L2031/7496 ,  B29L2031/7506 ,  B29L2031/756 ,  B81B3/0021 ,  B81B2201/036 ,  B81B2201/054 ,  B81B2203/0127 ,  B81C1/00103 ,  B81C1/00158 ,  F04B43/028 ,  F04B43/043 ,  F04B43/06 ,  F15C1/08 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0021 ,  F16K99/0034 ,  F16K99/0055 ,  F16K99/0057 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T29/49405 ,  Y10T137/212 ,  Y10T137/2202 ,  Y10T137/2218 ,  Y10T137/8593 ,  Y10T137/85978 ,  Y10T156/1002 ,  Y10T156/1043 ,  B29K2033/12 ,  B29K2025/06

摘要： Plastic microfluidic structures having a substantially rigid diaphragm that actuates between a relaxed state wherein the diaphragm sits against the surface of a substrate and an actuated state wherein the diaphragm is moved away from the substrate. As will be seen from the following description, the microfluidic structures formed with this diaphragm provide easy to manufacture and robust systems, as well readily made components such as valves and pumps.

公开(公告)号：USD681807S1

公开(公告)日：2013-05-07

申请号：US29381620

申请日：2010-12-21

申请人： R. Sam Niedbala ,  Lincoln C. Young ,  Peng Zhou ,  Matthew Sommerfield ,  Jarrett Seng

设计人： R. Sam Niedbala ,  Lincoln C. Young ,  Peng Zhou ,  Matthew Sommerfield ,  Jarrett Seng

公开(公告)号：US20120128549A1

公开(公告)日：2012-05-24

申请号：US13331211

申请日：2011-12-20

申请人： Peng Zhou ,  Lincoln C. Young

发明人： Peng Zhou ,  Lincoln C. Young

IPC分类号： B01L3/00

CPC分类号： F04B43/043 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0633 ,  B01L2400/0655 ,  B01L2400/0666 ,  B01L2400/084 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0084 ,  F16K2099/0094 ,  G01N21/5907

摘要： The systems and methods disclosed herein include a microfluidic system, comprising a pneumatic manifold having a plurality of apertures, and a chip manifold having channels disposed therein for routing pneumatic signals from respective ones of the apertures to a plurality of valves in a microfluidic chip, wherein the channels route the pneumatic signals in accordance with a configuration of the plurality of valves in the microfluidic chip.

摘要翻译： 本文公开的系统和方法包括微流体系统，其包括具有多个孔的气动歧管和具有设置在其中的通道的芯片歧管，用于将来自相应孔的气动信号路由到微流体芯片中的多个阀，其中 通道根据微流体芯片中的多个阀的结构来传送气动信号。

公开(公告)号：US20110293489A1

公开(公告)日：2011-12-01

申请号：US13189787

申请日：2011-07-25

申请人： Peng Zhou ,  Lincoln C. Young

发明人： Peng Zhou ,  Lincoln C. Young

IPC分类号： B01L3/00

CPC分类号： G01N1/28 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0633 ,  B01L2400/0655 ,  B01L2400/0666 ,  B01L2400/084 ,  F04B43/043 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T137/0324 ,  Y10T436/117497 ,  Y10T436/2575

摘要： The systems and methods described herein include a microfluidic chip having a plurality of microfeatures interconnected to provide a configurable fluid transport system for processing at least one reagent. Inserts are provided to removably interfit into one or more of the microfeatures of the chip, wherein the inserts include sites for interactions with the reagent. As will be seen from the following description, the microfluidic chip and the inserts provide an efficient and accurate approach for conducting parallel assays.

公开(公告)号：US08057629B2

公开(公告)日：2011-11-15

申请号：US12539797

申请日：2009-08-12

申请人： Peng Zhou ,  Lincoln C. Young

发明人： Peng Zhou ,  Lincoln C. Young

IPC分类号： B32B38/04

CPC分类号： B32B37/0038 ,  B01F5/0683 ,  B01F5/0688 ,  B01F11/0071 ,  B01F13/0059 ,  B01L3/502707 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/084 ,  B29C65/4895 ,  B29C66/1122 ,  B29C66/54 ,  B29C66/543 ,  B29C66/71 ,  B29C66/83411 ,  B29C66/8362 ,  B29L2031/756 ,  F04B43/043 ,  F04B43/06 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0021 ,  F16K99/0034 ,  F16K99/0055 ,  F16K99/0057 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T156/1002 ,  B29K2069/00 ,  B29K2033/08 ,  B29K2025/06

摘要： A method for making a polymeric microfluidic structure in which two or more components (layers) of the microfluidic structure are fixedly bonded or laminated with a weak solvent bonding agent, particularly acetonitrile or a mixture of acetonitrile and alcohol. In an aspect, acetonitrile can be used as a weak solvent bonding agent to enclose a microstructure fabricated in or on a non-elastomeric polymer such as polystyrene, polycarbonate, acrylic or other linear polymer to form a three-dimensional microfluidic network. The method involves the steps of wetting at least one of the opposing surfaces of the polymeric substrate components with the weak solvent bonding agent in a given, lower temperature range, adjacently contacting the opposing surfaces, and thermally activating the bonding agent at a higher temperature than the lower temperature range for a given period of time. The contacted polymeric substrates may also be aligned prior to thermal activation and compressed during thermal activation. A laminated, polymeric microfluidic structure is also disclosed.

摘要翻译： 一种制备聚合微流体结构的方法，其中微流体结构的两个或多个组分（层）用弱溶剂粘合剂，特别是乙腈或乙腈和醇的混合物固定粘合或层压。 在一个方面，乙腈可以用作弱溶剂粘合剂以包围在非弹性聚合物如聚苯乙烯，聚碳酸酯，丙烯酸或其它线性聚合物中或其上制造的微结构，以形成三维微流体网络。 该方法包括以下步骤：在给定的较低温度范围内用弱溶剂粘合剂润湿聚合物基材组分的至少一个相对表面，相邻接触相对表面，并在比 在一定时间内较低的温度范围。 接触的聚合物基材还可以在热活化之前对准，并在热活化期间被压缩。 还公开了层压聚合物微流体结构。

公开(公告)号：US20110272093A1

公开(公告)日：2011-11-10

申请号：US13185619

申请日：2011-07-19

申请人： Peng Zhou ,  Lincoln C. Young

发明人： Peng Zhou ,  Lincoln C. Young

IPC分类号： B32B37/20

CPC分类号： B32B37/0038 ,  B01F5/0683 ,  B01F5/0688 ,  B01F11/0071 ,  B01F13/0059 ,  B01L3/502707 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/084 ,  B29C65/4895 ,  B29C66/1122 ,  B29C66/54 ,  B29C66/543 ,  B29C66/71 ,  B29C66/83411 ,  B29C66/8362 ,  B29L2031/756 ,  F04B43/043 ,  F04B43/06 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0021 ,  F16K99/0034 ,  F16K99/0055 ,  F16K99/0057 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T156/1002 ,  B29K2069/00 ,  B29K2033/08 ,  B29K2025/06

摘要： A method for making a polymeric microfluidic structure in which two or more components (layers) of the microfluidic structure are fixedly bonded or laminated with a weak solvent bonding agent, particularly acetonitrile or a mixture of acetonitrile and alcohol. In an aspect, acetonitrile can be used as a weak solvent bonding agent to enclose a microstructure fabricated in or on a non-elastomeric polymer such as polystyrene, polycarbonate, acrylic or other linear polymer to form a three-dimensional microfluidic network. The method involves the steps of wetting at least one of the opposing surfaces of the polymeric substrate components with the weak solvent bonding agent in a given, lower temperature range, adjacently contacting the opposing surfaces, and thermally activating the bonding agent at a higher temperature than the lower temperature range for a given period of time. The contacted polymeric substrates may also be aligned prior to thermal activation and compressed during thermal activation. A laminated, polymeric microfluidic structure is also disclosed.

摘要翻译： 一种制备聚合微流体结构的方法，其中微流体结构的两个或多个组分（层）用弱溶剂粘合剂，特别是乙腈或乙腈和醇的混合物固定粘合或层压。 在一个方面，乙腈可以用作弱溶剂粘合剂以包围在非弹性聚合物如聚苯乙烯，聚碳酸酯，丙烯酸或其它线性聚合物中或其上制造的微结构，以形成三维微流体网络。 该方法包括以下步骤：在给定的较低温度范围内用弱溶剂粘合剂润湿聚合物基材组分的至少一个相对表面，相邻接触相对表面，并在比例 在一定时间内较低的温度范围。 接触的聚合物基材还可以在热活化之前对准，并在热活化期间被压缩。 还公开了层压聚合物微流体结构。